Skip to main content
SpringerLink
Log in

Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures — The factors affecting the cytokinin response

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Summary

Cytokinins were found to stimulate the alkaloid synthesis induced by removing auxin from the medium of a cell line of Catharanthus roseus. Diluting the mineral salts of the culture medium decreased the alkaloid production but increased the “sensitivity” of the cells. Addition of high levels of Ca2+, Mg2+ or Sr2+ to B5 media in which the mineral salts were diluted to 5–40%, increased the alkaloid production. The latter effect is related only partially to enhanced osmotic potential.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

CK:

cytokinin

2,4D:

2,4-dichlorophenoxyacetic acid

dw:

dry weight

Kin:

6-furfurylaminopurine

TLC:

thin layer chromatography

SE:

standard error

Z:

trans-zeatin

References

  • Ambid C, Roustan JP, Nef C, Fallot (1982) In Fujiwara A (ed.): Plant tissue culture, JAPTC, Tokyo, pp 331–332

    Google Scholar 

  • Bailey CM, Nicholson H, Stafford A, Smart NJ (1986) Appl. Biochem. Biotechn. 12:215–227

    Google Scholar 

  • Davies PJ (1988) In: Davies PJ (ed.): Plant Hormones and their Role in Plant Growth and Development, Kluwer Academic Publishers, Dordrecht Boston London, pp. 1–11

    Google Scholar 

  • Frischknecht PM, Bättig M, Giger ER, Baumann TW (1986) In: Sommers DA, Gengenbach BG, Biesboer DD, Hackett WP, Green CE (eds): 6th International Congress Plant Tissue Cultures, Minneapolis, Abstract n∘229, p. 201

  • Gamborg OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158

    Google Scholar 

  • Ganapathi B, Kargi F (1990) J. Exp. Bot. 41:259–267

    Google Scholar 

  • Hino F, Okazaki M, Miura Y (1982) Agr. Biol. Chem. 46:2195–2202

    Google Scholar 

  • Kauss H (1987) Ann. Rev. Plant. physiol. 38:47–72

    Google Scholar 

  • Kayser H, Gemmich AR (1984) Z. Naturforsch 39c:50–54

    Google Scholar 

  • Knobloch KH, Berlin J (1980) Z. Naturforsch 35c:551–556

    Google Scholar 

  • Kodja H, Liu D, Mérillon JM, Andreu F, Rideau M, Chénieux JC (1989) C.R. Acad. Sci. 309 serie III: 453–458

    Google Scholar 

  • Lau O, Yang SF (1975) Plant physiol. 55:738–740

    Google Scholar 

  • Lees GL (1986) Plant cell Rep. 5:247–251

    Google Scholar 

  • Leshem Y (1987) Physiol. plant. 69:551–559

    Google Scholar 

  • Marigo G, Rona JP, Pennarum AM, Boudet AM (1988) In: Production of metabolites by plant cell cultures, APRIA, Paris, pp 59–85

    Google Scholar 

  • Mérillon J.M., Chénieux J.C. and Rideau M., (1983) Planta Medica: 47, 169–176

    Google Scholar 

  • Mérillon JM, Ouelhazi L, Doireau P, Chénieux JC, Rideau M (1989) J. Plant physiol. 134:54–60

    Google Scholar 

  • Mérillon JM, Liu D, Huguet F, Chénieux JC, Rideau M (1991) Plant Physiol. Biochem. 29:289–296

    Google Scholar 

  • Nagakawa K, Konagai A, Fukui H, Tabata M (1984) Plant Cell Rep. 3:254–257

    Google Scholar 

  • Ozeki Y, Komamine A (1986) Plant Cell Physiol. 27:1361–1368

    Google Scholar 

  • Renaudin JP, Brown SC, Guem J (1985) In: Neumann KH, Barz W, Reinhard E (eds): Primary and secondary metabolism of plant cell tissues, Springer-Verlag, Berlin, pp 124–132

    Google Scholar 

  • Schütte HR, Liebisch HW (1985) In: Mothes K, Schütte HR, Luckner M (eds): Biochemistry of alkaloids, VEB Deutscher Verlag der Wissenschaften, Berlin, pp 188–271

    Google Scholar 

  • Smith JI, Smart NJ, Kurz WGW, Misawa M (1987) J Exp. Bot. 38:1501–1506

    Google Scholar 

  • Van der Heijden R, Verpoorte R, Ten Hoopen HJG (1989) Plant Cell Tissue and Organ Culture 18:231–280

    Google Scholar 

  • Zhao Z., Ross CW (1989) Plant Cell Physiol. 30:793–800

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Biochimie Végetale, Faculté de Pharmacie, Université de Tours, 2bis Bd Tonnellé, F-37042, Tours, France

    Alain Decendit, Di Liu, Lazhar Ouelhazi, Jean-Michel Mérillon & Marc Rideau

  2. Laboratoire de Physiologie Végétale, Faculté des Sciences, Université de Tours, France

    Pierre Doireau

Authors
  1. Alain Decendit
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Di Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lazhar Ouelhazi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre Doireau
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jean-Michel Mérillon
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Marc Rideau
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Barz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Decendit, A., Liu, D., Ouelhazi, L. et al. Cytokinin-enhanced accumulation of indole alkaloids in Catharanthus roseus cell cultures — The factors affecting the cytokinin response. Plant Cell Reports 11, 400–403 (1992). https://doi.org/10.1007/BF00234369

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234369

Keywords

Search

Navigation