Abstract
A method is described for the isolation and culture of protoplasts from cotyledon mesophyll cells of the tomato cultivar ‘Nadja’ grown axenically. Tissue cultures were produced from which shoots could be recovered. Rooted plantlets grew to normal plants and finally produced mature fruits.
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Abbreviations
- IAA:
-
indoleacetic acid
- IBA:
-
indolebutyric acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- BA:
-
benzyladenine
- GA3 :
-
gibberellic acid
- MS:
-
Murashige and Skoog (1962) medium
- B5:
-
Gamborg et al. (1968) medium
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Dedicated with sincere compliments to Professor Dr.Dr.h.c.mult. Hans Stubbe on the occasion of his 80th birthday
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Koblitz, H., Koblitz, D. Experiments on tissue culture in the genus Lycopersicon miller. Plant Cell Reports 1, 143–146 (1982). https://doi.org/10.1007/BF00269183
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DOI: https://doi.org/10.1007/BF00269183