Skip to main content
SpringerLink
Log in

Cloning of photoreactivation repair gene and excision repair gene of the yeast Saccharomyces cerevisiae

  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

The photoreactivation repair gene (PHR1) of the yeast Saccharomyces cerevisiae was cloned in a hybrid plasmid (pJDB207), which is able to replicate as a multicopy episome in S. cerevisiae and Escherichia coli cells. The size of the DNA fragment found to have the photoreactivation activity was 3.0 kb, determined by recloning of the isolated fragment. In wild type cells transformed by the plasmid containing the PHR1 gene, the number of DNA photolyase molecules was 15 times greater than in wild type cells with pJDB207 only. Using the same receptor strain the excision repair gen RAD1 was also isolated. The size of the insert of the DNA which complements excision repair deficiency in recipient yeast cells was 5.7 kb. The recipient cells after transformation with the plasmid containing RAD1 showed the same UV-sensitivty as wild type cells with pJDB207 only.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

UV:

Ultra-violet light of 254 run wavelength

References

  • Beggs JD (1978) Nature (London) 275:104–109

    Google Scholar 

  • Chevallier MR, Bloch JC, Lacroute F (1980) Gene 11:11–19

    Google Scholar 

  • Clewell DB (1972) J Bacteriol 110:667–676

    Google Scholar 

  • Cohen SN, Chiang ACY, Hsu L (1972) Proc Natl Acad Sci USA 69:2110–2114

    Google Scholar 

  • Hanawalt PC, Cooper PK, Ganesen AK, Smith CA (1979) Annu Rev Biochem 48:783–836

    Google Scholar 

  • Harm W, Rupert CS, Harm H (1971) In: Giese AC (ed) Photophysiology. Academic Press, New York London, pp 279–324

    Google Scholar 

  • Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Aca USA 75:1929–1933

    Google Scholar 

  • Losson R, Lacroute F (1981) Mol Gen Genet 184:394–399

    Google Scholar 

  • Resnick MA (1969) Photochem Photobiol 9:307–312

    Google Scholar 

  • Reynolds RJ, Love JD, Friedberg EC (1981) J Bacteriol 147:705–708

    Google Scholar 

  • Rupert CS (1975) In: Hanawalt PC, Setlow RB (ed) Molecular Mechanisms for Repair of DNA. Plenum Press, New York, pp 73–87

    Google Scholar 

  • Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76:1035–1039

    Google Scholar 

  • Yasui A, Laskowski W (1975) Int J Radial Biol 28:511–518

    Google Scholar 

  • Yasui A (1976) PhD Thesis, Freie Universität Berlin

Download references

Author information

Authors and Affiliations

  1. Medical Biological Laboratory, TNO, 139, Lange Kleiweg, The Netherlands

    Akira Yasui

  2. Laboratoire de Genetique Physiologique, Institut de Biologie, Moleculaire et Cellulaire du Centre National de la Recherche Scientifique, 67084, Strasbourg Cedex, France

    Marie-Renee Chevallier

Authors
  1. Akira Yasui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marie-Renee Chevallier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yasui, A., Chevallier, MR. Cloning of photoreactivation repair gene and excision repair gene of the yeast Saccharomyces cerevisiae . Curr Genet 7, 191–194 (1983). https://doi.org/10.1007/BF00434889

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00434889

Key words

Search

Navigation