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Isolation and molecular analysis of the gene for cytochrome c1 from Kluyveromyces lactis

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Abstract  

By ethyl methanesulphonate mutagenesis of the yeast Kluyveromyces lactis we have isolated five nuclear mutants that were unable to grow on non-fermentable carbon sources. The mutations were found to belong to three complementation groups. After functional complementation of the mutation in one of these mutants we have cloned the structural gene for cytochrome c 1, named KlCYT1. This gene has been assigned to chromosome VI and its nucleotide sequence exhibited 74.3% identity to the homologous gene of S. cerevisiae.

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Authors and Affiliations

  1. Department of Microbiology and Virology, Comenius University, Mlynská dolina B-2, 842 15 Bratislava, Slovak Republic, , , , , , XX

    Yvetta Gbelská, Katarína Horváthová & Július Šubík

  2. Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 Al Leiden, The Netherlands, , , , , , NL

    Quirina J. M. van der Aart, B. J. M. Zonneveld & H. Y. Steensma

Authors
  1. Yvetta Gbelská
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  2. Katarína Horváthová
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  3. Quirina J. M. van der Aart
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  4. B. J. M. Zonneveld
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  5. H. Y. Steensma
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  6. Július Šubík
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Received: 6 February 1996 / 2 April 1996

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Gbelská, Y., Horváthová, K., van der Aart, Q. et al. Isolation and molecular analysis of the gene for cytochrome c1 from Kluyveromyces lactis. Curr Genet 30, 145–150 (1996). https://doi.org/10.1007/s002940050113

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  • DOI: https://doi.org/10.1007/s002940050113

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