Abstract
Replicative plasmids generated through in-vivo recombination have been identified among transformants of the fungus Pleurotus ostreatus. In addition to sequences from a standard selection vector (pAN7-1), these recombinant plasmids contain recombined sequences of chromosomal origin conferring replicative potential upon the vector. One such recombined sequence, an 1148-bp insert into plasmid pP01, has been characterized. This sequence has been analyzed for secondary structural features as well as for consensus sites affiliated with origins of replication (ori) in other eukaryotic systems. The 1148-bp insert lacks an ORF and does not contain an acceptable match to the commonly identified 11-bp ars consensus sequence (A/TTTTATA/GTTTA/T) for autonomous replication in the yeast Saccharomyces cerevisiae. The analysis, however, revealed a cluster of three hairpin-loop-forming subsequences with individual ΔG25°C free energy values of-7.6,-6.4 and-5.2 kcal mol-1. Also found were two 7-bp analogues to centromere-affiliated sequences recognized in other fungi, as well as several putative gyrase recognition sites comparable to the 9-bp S. cerevisiae/E. coli gyrase-binding consensus sequence. Sequences comparable to the ori of the yeast 2-μm plasmid or to various sequences associated with ori of yeast/fungal mitochondrial DNAs (mtDNA) were not present in the 1148-bp insert. Replication of pP01 appears rather to involve a replication of chromosomal derivation devoid of an ars-type consensus.
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References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1989) Short protocols in molecular biology. John Wiley, New York
Baldacci G, Cherif-Zahan B, Bernardi G (1984) EMBO J 3:2115–2120
Ballance DJ (1991) Transformation systems for filamentous fungi and an overview of fungal gene structure. In: Leong SA, Berka RM (eds) Molecular industrial mycology. Marcel Dekker, New York, pp 1–29
Ballance DJ, Turner G (1985) Gene 36:321–331
Beggs JD (1978) Nature 275:104–109
Bej A, Perlin MH (1991) Gene 98:135–140
Brewer BJ, Fangman WL (1987) Cell 51:463–471
Buxton FP, Radford A (1984) Mol Gen Genet 196:339–344
Campbell JL, Newlon CS (1991) Chromosomal DNA replication. In: Broach JR, Pringle JR, Jones EW (eds) The molecular biology of the yeast Saccharomyces: genome dynamics, protein synthesis, and energetics. Cold Spring Harbor Laboratory Press, Plainview, pp 41–146
Case ME (1982) Transformation of Neurospora crassa utilizing recombinant plasmid DNA. In: Hollaender A, DeMoss RD, Kaplan S, Konisky J, Savage D, Wolfe RS (eds) Genetic engineering of microorganisms for chemicals. Plenum Press, New York, pp 87–100
Chang DD, Hanswirth WW, Clayton DA (1985) EMBO J 4:1559–1567
Churchill ACL, Ciuffetti LA, Hansen DR, Van Etten HD, Van Alfen NK (1990) Curr Genet 17:25–31
Clarke L, Carbon J (1980) Proc Natl Acad Sci USA 77:2173–2177
Cullen D, Leong SA, Wilson LJ, Henner DJ (1987a) Gene 57:21–26
Cullen D, Wilson LJ, Grey GL, Henner DJ, Turner G, Ballance DJ (1987b) Nucleic Acids Res 15:9163–9175
Diffley JFX, Cocker JH (1992) Nature 357:169–172
Edman JC (1992) Mol Cell Biol 12:2777–2783
Esser K, Kuck U, Lang-Hinrichs C, Lemke P, Osiewacz HD, Stahl U, Tudzynski P (1986) Plasmids of eukaryotes. Springer-Verlag, Berlin
Fangman WL, Brewer BJ (1991) Annu Rev Cell Biol 7:375–403
Fincham JRS (1989) Microbiol Rev 53:148–170
Finkelstein DB (1992) Transformation. In: Finkelstein DB, Ball C (eds) Biotechnology of filamentous fungi; technology and products. Butterworth-Heinemann, Stoneham, pp 113–156
Fujimura H, Hishinuma F, Gunge N (1987) Curr Genet 12:99–104
Gems D, Johnstone IL, Clutterbuck AJ (1991) Gene 98:61–67
Goldman GH, Geremia R, Van Montagu M, Herrea-Estrella A (1990) Transformation of filamentous fungi by high voltage electroporation. In: Bio-Rad Bulletin 1352, Bio-Rad Laboratories, Richmond, California, pp 1–4
Grant DM, Lambowitz AM, Rambosek JA, Kinsey JA (1984) Mol Cell Biol 4:2041–2051
Gritz L, Davies J (1983) Gene 25:179–188
Gunge N (1988) Kluveromyces linear DNA plasmids. In: Koltin Y, Leibowitz MJ (eds) Viruses of fungi and simple eukaryotes. Marcel Dekker, New York, pp 265–282
Haas JM de, Hille J, Kors F, van der Meer B, Kool AJ, Folkerts O, Nijkamp HJJ (1991) Curr Genet 20:503–513
Hanahan D (1983) J Mol Biol 166:557–580
Hartley JL, Donelson JE (1980) Nature 286:860–864
Heeswijck R van (1986) Carlsberg Res Commun 51:433–443
Hieter P, Pridmore D, Hegermann JH, Thomas M, Davis RW and Philipsen P (1985) Cell 42:913–921
Hinnen A, Hicks JB, Fink GR (1978) Proc Natl Acad Sci USA 75:1929–1933
Hondel CAMJJ van den, Punt PJ (1991) Gene-transfer systems and vector development for filamentous fungi. In: Peberdy JF, Caten CE, Bennett JW (eds) Applied molecular genetics of fungi. Cambridge University Press, Cambridge, pp 1–28
Innis MA, Gelfand DH, Sninsky JJ, White TJ (1990) PCR protocols: a guide to methods and application. Academic Press, San Diego
Kirchner G, Roberts JL, Gustafson GD, Ingolia TD (1989) Gene 81:349–354
Kornberg A, Baker TA (1992) DNA replication. W. H. Freeman and Company, New York
Liebman SW, Picologlou S (1988) Recombination associated with yeast retrotransposons. In: Koltin Y, Leibowitz MJ (eds) Viruses of fungi and simple eukaryotes. Marcel Dekker, New York, pp 63–90
Marahrens Y, Stillman B (1992) Science 255:817–823
Mooibroek H, Kuipers AGJ, Sietsma JH, Punt PJ, Wessels JGH (1990) Mol Gen Genet 222:41–48
Murray AM, Szostak JW (1983) Nature 305:189–193
Natale AD, Schubert AE, Kowalski D (1992) Proc Natl Acad Sci USA 89:2654–2658
Paietta J, Marzluf GA (1985) Curr Genet 9:383–388
Peng M, Singh NK, Lemke PA (1992) Curr Genet 22:53–59
Perlin MH, Bej AK, Will OH, Jacob RJ (1990) J Indust Microbiol 5:355–364
Perrot M, Barreau C, Begueret J (1987) Mol Cell Biol 7:1725–1730
Powell WA, Kistler HC (1990) J Bacteriol 172:3163–3171
Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene 56:117–124
Randall T, Rao T, Reddy CA (1989) Biochem Biophys Res Commun 161:720–725
Randall T, Reddy CA, Boominathan K (1991) J Bacteriol 173:776–782
Randall TA, Reddy CA (1992) Curr Genet 21:255–260
Revuelta JL, Jayaram M (1986) Proc Natl Acad Sci USA 83:7344–7347
Rivier DH, Rine J (1992) Science 256:659–663
Roberts IN, Oliver RP, Punt PJ, van den Hondel CAMJJ (1989) Curr Genet 15:177–180
Samac DA, Leong SA (1989) Curr Genet 16:187–194
Snyder M, Sapolsky RJ, Davis RW (1988) Mol Cell Biol 8:2184–2194
Som T, Armstrong KA, Volkert FC, Broach JR (1988) Cell 52:27–37
Southern EM (1975) J Mol Biol 98:503–517
Stahl U, Tudzynski P, Kuck U, Esser K (1982) Proc Natl Acad Sci USA 79:3641–3645
Stohl LL, Lambowitz AM (1983) Proc Natl Acad Sci USA 80:1058–1062
Struhl K, Stinchcomb DT, Scherer S, Davis RW (1979) Proc Natl Acad Sci USA 76: 1035–1039
Tilburn J, Scazzochio C, Taylor GG, Zabicky-Zissman JH, Lockington RA and Davies RW (1983) Gene 26:205–221
Tsukuda T, Carleton S, Fotheringham S, Holloman WK (1988) Mol Cell Biol 8:3703–3709
Williamson DH (1985) Yeast 1:1–14
Wong TW, Claton DA (1985) Cell 42:951–958
Wostemeyer J, Burmester A, Weigel C (1987) Curr Genet 12:625–627
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Peng, M., Lemke, P.A. & Singh, N.K. A nucleotide sequence involved in replicative transformation of a filamentous fungus. Curr Genet 24, 114–121 (1993). https://doi.org/10.1007/BF00324674
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DOI: https://doi.org/10.1007/BF00324674