Summary
TheERG10 gene specific toS. uvarum, a brewing yeast, has been cloned by complementation of anS. cerevisiae erg10 mutant.S. uvarum contains two differentERG10 genes. One of these is similar to theS. cerevisiae ERG10 gene; they are structurally different, but functionally homologous. The clonedERG10 gene has been located on chromosome XVI, and we have shown that it is allelic to the previously isolatedtsm0115 mutants. Northern blot and sequence analysis indicate that theERG10 gene is highly expressed, and biochemical and genetic evidence show that it encodes the cytoplasmic acetoacetyl CoA thiolase.
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References
Bennetzen JL, Hall BD (1982) J Biol Chem 257:3026–3031
Boutelet F (1982) PhD thesis, University of Gembloux, Belgium
Carle GF, Olson MV (1984) Nucleic Acids Res 12:5647–5664
Carlson M, Botstein D (1982) Cell 28:145–154
Casey GP (1986) Carlsberg Res Commun 51:327–341
Cohen SN, Chang ACY, Hsu L (1972) Proc Natl Acad Sci USA 69:2110–2114
Dale RMK, McLure BA, Houchins JP (1985) Plasmid 13:31–40
Dobson MJ, Tuite MF, Roberts NA, Kingsman AJ, Kingsman SM (1982) Nucleic Acids Res 10:2625–2637
Gehring U, Harris JI (1970) Eur J Biochem 16:492–498
Gerbaud C, Elmerich C, Tandeau de marsac N, Chocat P, Charpin N, Guerineau M, Auber JP (1981) Curr Genet 3:173–181
Hahn S, Hoar ET, Guarente L (1985) Proc Natl Acad Sci USA 82:8562–8566
Hicks JB, Strathern JN, Klar ASJ, Dellaporta SL (1982) In: Setlow JK, Hollaender A (eds) Genetic engineering USA 4: 219–248
Hilger F, Prevot M, Mortimer R (1982) Curr Genet 6:93–98
Holmberg S (1982) Carlsberg Res Commun 47:233–244
Hurt EC, Van Loon APGM (1986) TIBS II:204–207
Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168
Kornblatt JA, Rudney H (1971a) J Biol Chem 246:4417–4423
Kornblatt JA, Rudney H (1971b) J Biol Chem 246:4424–4430
Maniatis T, Fritsh EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Messing J (1983) In: Wu R, Grossman L, Moldave K (eds) Methods in enzymology, vol 101. Academic Press, New York, pp 20–49
Myers AM, Tzagoloff A (1985) J Biol Chem 260:15371–15377
Natsoulis G, Hilger F, Fink GR (1986) Cell 46:235–243
Naumovski L, Friedberg EC (1983) Gene 22:203–209
Nilsson-Tillgren T, Gjermansen C, Holmberg S, Petersen JGL (1986) Carlsberg Res Commun 51:309–326
Pedersen MB (1985) Carlsberg Res Commun 180:263–272
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Schleif RF, Wensink PC (1981) In: Pract Meth in Mol Biol, vol 74. Springer, Vienna New York
Servouse M, Mons N, Baillargeat JL, Karst F (1984) Biochem Biophys Res Commun 123:424–430
Servouse M, Karst F (1986) Biochem J 240:541–547
Southern EM (1975) J Mol Biol 98:503–517
Stewart PR, Rudney H (1966) J Biol Chem 241:1212–1221
Trocha PJ, Sprinson DB (1976) Arch Biochem Biophys 174:45–51
Van Soligen P, Van der Plaat JB (1977) J Bacteriol 130:946–947
Wickner RB, Boutelet F, Hilger F (1983) Mol Cell Biol 3:415–420
Zaret KS, Sherman F (1982) Cell 28:563–573
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Dequin, S., Gloeckler, R., Herberte, C.J. et al. Cloning, sequencing and analysis of the yeastS. uvarum ERG10 gene encoding acetoacetyl CoA thiolase. Curr Genet 13, 471–478 (1988). https://doi.org/10.1007/BF02427752
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DOI: https://doi.org/10.1007/BF02427752