Summary
Neurospora crassa wild type genome shows DNA sequences which are homologous to the sequences present in the rRNA processing genes of the yeast Saccharomyces cerevisiae. Five such processing genes from yeast, viz., RNA1 through RNA5, cloned in plasmid pBR322 were transformed in Escherichia coli strain LE392. Southern blots containing DNAs from these clones were restricted with several restriction endonucleases along with DNAs from lambda phage, rice (plant) and neuroblastoma (animal), were hybridized with 32P-labelled nick-translated N. crassa nuclear DNA under very stringent conditions. Autoradiograms of these blots revealed that four yeast rRNA processing genes (RNA1, RNA2, RNA3, and RNA4) showed homology with N. crassa nuclear DNA but such analogs were not found in DNAs representing prokaryotes, phages, higher plants and animals.
References
Atkinson NS, Dunst RW, Hopper AK (1985) Mol Cell Biol 5:907–915
Dutta SK, Ghosh B, Chambers C, Verma M (1986) The Nucleus 29:9–20
Hopper AK (1984) In: Apirion D (ed) Prcessing of RNA. CRC Press, Boca Raton, Forida
Hopper A, Blanks F, Evangelids V (1978) Cell 14:211–219
Last RL, Woolford JL (1985) Genetics 110 (Abstr):67s
Last RL, Stavenhagen JB, Woolford JL (1984) Mol Cell Biol 4:2396–2405
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory mannual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Verma M, Dutta SK (1987) Curr Genet 11:309–314
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dutta, S.K., Verma, M. & Verma, M. Neurospora crassa nuclear genome contains analogy of Saccharomyces cerevisiae genes for ribosomal RNA processing. Curr Genet 12, 225–229 (1987). https://doi.org/10.1007/BF00436884
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00436884