Summary
Magnetic resonance techniques yield important information on different coordination behaviour of Mn(II) in the presence of biomacromolecules. A preliminary study on model systems is necessary to have a deeper insight on dynamics and structural aspects.
The EPR technique is not able to identify a binding interaction between Trp and manganous ion when the metal is bonded to the Imidazole. Proton shift NMR studies on the Mn(II)-5′ATP-Imid complex evidenziate a ternary system formation and a stacking interaction between the imidazole-ring and the nucleotide purine-moiety.
An EPR and NMR combined analysis on the Mn(II)-5′ATP-Trp system points out a stacking interaction and the simultaneous presence of a covalent binding between the metal ion and the biomolecules. The role of the metal ion to favour this coordination is suggested.
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Basosi, R., Laschi, F. & Rossi, C. Effectiveness and limits of magnetic resonance techniques in the identification of different coordination pathways of the Mn(II) in the presence of biomacromolecules. Polymer Bulletin 5, 263–269 (1981). https://doi.org/10.1007/BF00254712
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DOI: https://doi.org/10.1007/BF00254712