Summary
Whole cells of Pseudomonas fluorescens which utilized mainly glucose were immobilized in collagen membrane. The microbial electrode consisted of a bacteria-collagen membrane and an oxygen electrode was developed for the determination of glucose. When the electrode was inserted in a sample solution containing glucose, the current of the electrode decreased markedly with time until a steady state was reached. The response time of the electrode was 10 min by the steady state method. A linear relationship was observed between the steady state current and the concentration of glucose below 20 mg l −1. The minimum concentration for determination was 2 mg of glucose per liter. The reproducibility of the current was examined using the same sample solution. The current was reproducible within ±6% of the relative error when a sample solution containing 10 mg {ie343-1} of glucose was employed. The standard deviation was 0.6 mg {ie343-2} in 20 experiments. The reusability of the glucose sensor was examined using the same sample solution (10 mg {ie343-3}). No decrease in current output was observed over a two week period and 150 assays. Glucose in molasses was determined with an average relative error of 10% by the microbial electrode sensor.
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Karube, I., Mitsuda, S. & Suzuki, S. Glucose sensor using immobilized whole cells of Pseudomonas fluorescens . European J. Appl. Microbiol. Biotechnol. 7, 343–350 (1979). https://doi.org/10.1007/BF00499848
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DOI: https://doi.org/10.1007/BF00499848