Summary
Accumulation of uranium (U) is reported for isolated exopolysaccharide produced by Pseudomonas sp. EPS-5028. A maximum uptake of 96 μg U/mg polymer was observed. In contrast, the maximum accumulation of uranium by deacylated polysaccharide was 46 μg/mg. This metal-complexing capacity observed suggests that the anionic reactive sites on the structure could be responsible for this activity. Metal uptake was affected by pH and was not affected by temperature. Expolysaccharide from Pseudomonas sp. EPS-5028 obeyed the Freundlich isotherm indicating single layer adsorption.
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References
Brown MJ, Lester JN (1979) Metal removal in activated sludge: the role of bacterial extracellular polymers. Water Res 13:817–837
Congregado F, Estañol I, Espuny MJ, Fuste MC, Manresa MA, Marqués AM, Guinea J, Simon-Pujol MD (1985) Preliminary studies on the production and composition of the extracellular polysaccharide synthesized by Pseudomonas sp. EPS-5028. Biotechnol Lett 7:883–888
DiSpirito AA, Talnagi JW, Tuovinen OH (1983) Accumulation and cellular distribution of uranium in Thiobacillus ferrooxidans. Arch Microbiol 135:250–253
Farrah SR, Unz RF (1976) Isolation of exocellular polymer from Zoogloea strains MP6 and 106 and from activated sludge. Appl Environ Microbiol 32:33–37
Friss N, Myers-Keith P (1986) Biosorption of uranium and lead by Streptomyces longwoodensis. Biotechnol Bioeng 28:21–28
Fusté MC, Simon-Pujol MD, Marqués AM, Guinea J, Congregado F (1986) Isolation of a new free-living bacterium containing R-bodies. J Gen Microbiol 132:2801–2805
Horikoshi T, Nakajima A, Sakagushi T (1981) Studies on the accumulation of heavy metal elements in biological systems. XIX. Accumulation of uranium by microorganisms. Eur J Appl Microbiol Biotechnol 12:90–96
Jeanes A, Rogovin P, Cadmus MC, Silman RW, Knutson CA (1976) ARS-NC-51 U.S. Department of Agriculture, Washington, D. C.
Kaplan D, Christianen D, Arad SM (1987) Chelating properties of extracellular polysaccharides from Chlorella spp. Appl Environ Microbiol 53:2953–2956
Knutson CA, Pittsley JE, Jeanes A (1979) Composition and properties of the extracellular polysaccharide produced by Arthrobacter stabilis NRRL B-3225. Carbohydr Res 73:159–168
Lester JN, Sterrit RM (1985) Microbial accumulation of heavy metals in wastewater treatment processes. J Appl Bacteriol Symp Suppl 59:141S–153S
Marqués AM, Estañol I, Alsina JM, Fusté MC, Simon-Pujol MD, Guinea J, Congregado F (1986) Production and rheological properties of the extracellular polysaccharide synthesized by Pseudomonas sp. strain EPS-5028. Appl Environ Microbiol 52:1221–1223
Scott JA, Palmer SJ (1988) Cadmium bio-sorption by bacterial exopolysaccharide. Biotechnol Lett 10:21–24
Stranberg GW, Shumate SE, Parrot JR (1981) Accumulation of uranium by Saccharomyces cerevisiae and Pseudomonas aeruginosa. Microbial cells as biosorbants for heavy metals. Appl Environ Microbiol 41:237–245
Volesky B (1987) Biosorbents for metal recovery. Tibtech 5:96–101
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Marqués, A.M., Bonet, R., Simon-Pujol, M.D. et al. Removal of uranium by an exopolysaccharide from Pseudomonas sp.. Appl Microbiol Biotechnol 34, 429–431 (1990). https://doi.org/10.1007/BF00170074
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DOI: https://doi.org/10.1007/BF00170074