Summary
An investigation was performed to recognise a fungus capable of 19-hydroxylating the steroids androstenedione, cortexolone and progesterone, in high yield with little or no side reactions. The fungi selected for study belong to a group which has been previously reported to possess 19-hydroxylation ability. Pellicularia filamentasa f. sp. microsclerotia IFO 6298 and P. filamentosa f.sp. sasakii IFO 5254 both 19-hydroxylated cortexolone, but 19-hydroxylated products were not detected using either androstenedione or progesterone as substrate. When 19-hydroxylating cortexolone, both strains of P. filamentosa produced 11β-hydroxycortexolone as a by-product, but P. filamentosa f.sp. microsclerotia IFO 6298 was superior in terms of 19-hydroxylation and the relative amounts of the two products.
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References
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Clark, T.A., Chong, R. & Maddox, I.S. An investigation into the 19-hydroxylation of androstenedione, cortexolone and progesterone by selected fungi. Appl Microbiol Biotechnol 21, 132–134 (1985). https://doi.org/10.1007/BF00252376
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DOI: https://doi.org/10.1007/BF00252376