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Production of steroid-ring-B-seco acid from β-sitosterol and influence of surfactants on sterol uptake in Nocardia sp. M 29–40

  • Industrial Microbiology
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Summary

The effect of several parameters (temperature; pH; main carbon source; time and amount of β-sitosterol addition; Tween 20, 40, 60, 80; Span 20; pluronic F 68, L 64) on the conversion of β-sitosterol to 3-(5α-hydroxy-7aβ-methyl-1-oxo-3aα-H-hexahydroindan-4α-yl) propionic acid (I) by Nocardia sp. M. 29–40 was investigated. A maximal theoretical yield of 65 mol% I (with respect to substrate added) could be achieved during cultivation at pH 8.0 in presence of 6 g/l Tween 40 or Tween 60. Tween 40 and Tween 60 stimulate β-sitosterol cooxidation not by improving the substrate suspension but by providing a fatty acid component as precursor for biosynthesis of surface active cell wall lipids.

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References

  • Ben-Bassat A, Goldberg I (1977) Biochim Biophys Acta 497: 586

    Google Scholar 

  • El-Refai AH, Sallam LAR, Naim N (1975) Z Allg Microbiol 15: 59

    Google Scholar 

  • Goodfellow M, Minniken DE, Patel PV, Mordarska H (1973) J Gen Microbiol 74: 185

    Google Scholar 

  • Goren MB (1972) Bacteriol Rev 36: 33

    Google Scholar 

  • Hartog J, Halkes SJ, Morsik L, Wachter Am De, Schlatmann ILMA (1975) J Steroid Biochem 6: 577

    Google Scholar 

  • Jankewitz H, Mehl E (1960) Hoppe Seyler's Z Physiol Chem 320: 251

    Google Scholar 

  • Knuppen R, Haupt O, Brauer H (1975) Drug Res 25: 28

    Google Scholar 

  • Martin CKA (1974) Dissertation, Technische Universität Braunschweig

  • Martin CKA, Wagner F (1976) Eur J Appl Microbiol 2: 243

    Google Scholar 

  • Minniken DE, Alshamaoni L, Goodfellow H (1975) Gen Microbiol 88: 200

    Google Scholar 

  • Nishikawa D, Imada Y, Kinoshita M, Takahashi K, Machida H Ger offen 2625 333, (4.6.76/16.12.75)

  • Probst M (1974) Dissertation, Technische Universität Braunschweig

  • Rapp P, Bock H, Urban E, Wagner F, Gebetsberger W, Schulz W (1977). Dechema-Monographie Biotechnologie, vol 81. Verlag Chemie, Weinheim, p 177

    Google Scholar 

  • Rapp P, Bock H, Wray V, Wagner F (1979) J Gen Microbiol 115: in press

  • Schömer U, Sheldrick WS, Wagner F (1978) JCS Perkin I, 336

  • Schubert K, Ritter F, Sorkina T, Böhme KH, Hörhold C (1975) J Steroid Biochem 6: 1501

    Google Scholar 

  • Stahl E, Kaltenbach U (1961) J Chromatogr 5: 351

    Google Scholar 

  • Strijewski A, Tan TL, Bozler G, Zahn W, Wagner F (1972) Z Physiol Chem 352: 1446

    Google Scholar 

  • Suzuki T, Tanaka K, Matsubara J, Kinoshita S (1969) Agric Biol Chem 33: 1619

    Google Scholar 

  • Zajic JE, Guignard H, Gerson DF (1977) Biotechnol Bioeng 19, 1285, 1303

    Google Scholar 

Download references

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Authors and Affiliations

  1. Lehrstuhl für Biochemie und Biotechnologie der Technischen Universität Braunschweig, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany

    Ulrike Schömer & Fritz Wagner

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  1. Ulrike Schömer
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  2. Fritz Wagner
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In memory of Professor David Perlmann

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Schömer, U., Wagner, F. Production of steroid-ring-B-seco acid from β-sitosterol and influence of surfactants on sterol uptake in Nocardia sp. M 29–40 . European J. Appl. Microbiol. Biotechnol. 10, 99–106 (1980). https://doi.org/10.1007/BF00504732

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  • DOI: https://doi.org/10.1007/BF00504732

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