Summary
The adsorption behaviour of cellulase fromTrichoderma viride on microcrystalline celluloses with different specific surface areas was studied. The adsorption was found to fit a Langmuir isotherm. There was an increase in the maximum adsorption amount (Amax) as the specific surface area of microcrystalline cellulose increased. The values of Amax and adsorption equilibrium constant (K) decreased with increasing temperature. Thermodynamic parameters in adsorption were calculated from K. It was found from the enthalpy of adsorption, that van der Waals-Type interaction was responsible for adsorption of cellulase on microcrystalline cellulose. The adsorption process was exothermic and an adsorption enthalpy-controlled reaction.
Similar content being viewed by others
References
Berghem LER, Pettersson LG, Axio-Fredriksson U-B (1975) The mechanism of enzymatic cellulose degradation. Eur J Biochem 53:55–62
Brown RD Jr (1982) Cellulose and other natural polymer systems. In: Whate AR (eds) Visualization of cellulases and cellulose degradation. Plenum, New York, pp 489–509
Castellan GW (1983) Physical chemistry, 3rd edn. Addison-Wesley, Reading, Mass.
Caulfield DF, Moore WE (1974) Effect of varying crystallinity of cellulose on enzymatic hydrolysis. Wood Sci 6:375–379
Gharpuray MM, Lee Y-H, Fan LT (1983) Structural modification of lignocellulosics by treatments to enhance enzymatic hydrolysis. Biotechnol Bioeng 21:131–146
Ghose TK, Bisaria VS (1979) Studies on the mechanism of enzymatic hydrolysis. Biotechnol Bioeng 31:131–146
Goel SC, Ramachandran KB (1983) Studies on the adsorption of cellulase on lignocellulosics. J Ferment Technol 61:281–286
Herr D, Luck G, Dellweg H (1978) Formation of cellulases and degradation of cellulose by several fungi. J Ferment Technol 56:273–278
Kim DW, Yang JH, Jung YG (1986) Hydrolysis of cellulose by enzyme mixture. J Korean Inst Chem Eng 24:407–413
Ladisch MR, Lin KW, Voloch M, Tsao GT (1983) Process considerations in the enzymatic hydrolysis of biomass. Enzyme Microb Technol 5:82–101
Lee YH, Fan LT (1982) Kinetic studies of enzymatic hydrolysis of insoluble cellulose: analysis of the initial rates. Biotechnol Bioeng 26:2383–2406
Lee SB, Ryu DDY, Taguchi T (1983) Structural properties of cellulose reaction mechanism. Biotechnol Bioeng 25:33–51
Lee SB, Shin HS, Ryu DDY, Mandels M (1982) Adsorption of cellulase on cellulose: effect of physicochemical properties of cellulose on adsorption and rate of hydrolysis. Biotechnol Bioeng 24:2137–2153
Lowry OH, Rosebrough NJ, Farr AE, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–273
Mandels M, Sternberg D (1976) Recent advances in cellulase technology. J Ferment Technol 54:267–286
Mandels M, Hontz L, Nystrom J (1974) Enzymatic hydrolysis of waste cellulose. Biotechnol Bioeng 16:1471–1493
Moloney A, Coughlan MP (1983) Sorption ofTalaromyces emersonii cellulase on cellulosic substrates. Biotechnol Bioeng 25:271–280
Ooshima H, Sakata M, Harano Y (1983) Adsorption of cellulase fromTrichoderma viride. Biotechnol Bioeng 25:3103–3114
Puri VP (1984) Effect of crystallinity and degree of polymerization of cellulose on enzymatic saccharification. Biotechnol Bioeng 26:1219–1222
Ryu DDY, Kim C, Mandels M (1984) Competitive adsorption of cellulase components and its significance in a synergistic mechanisms. Biotechnol Bioeng 26:488–496
Segal LJJ, Creeley AE, Martin JR, Conrad CM (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29:786–794
Summer JB, Somers GF (1944) Laboratory experiments in biological chemistry. Academic Press, New York, pp 34–37
Tjerneld F, Persson I, Albertsson P-A, Hagerdal BH (1985) Enzymatic hydrolysis of cellulose in aqueous two-phase systems. I. Partition of cellulase fromTrichoderma reesei. Biotechnol Bioeng 27:1036–1043
Vallander L, Eriksson K-E (1985) Enzymic saccharification of pretreated wheat straw. Biotechnol Bioeng 27:650–659
Wood TM, McGrae SI (1979) Synergism between enzymes involved in the solubilization of native cellulose. In: Brown RD Jr Jurasek L (eds) Hydrolysis of cellulose: mechanisms of enzymatic and acid catalysis. Adv Chem Ser 181, American Chemical Society, Washington DC, pp 181–209
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kim, D.W., Yang, J.H. & Jeong, Y.K. Adsorption of cellulase fromTrichoderma viride on microcrystalline cellulose. Appl Microbiol Biotechnol 28, 148–154 (1988). https://doi.org/10.1007/BF00694303
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00694303