Abstract
Using polymerase chain reaction, interleukin-6 (IL-6) cDNA fragments from harbor seal (Phoca vitulina), killer whale (Orcinus orca), and Southern sea otter (Enhydra lutris nereis) were cloned and sequenced. For all three species, a continuous open reading frame encoding 203 residues for harbor seal, 199 residues for killer whale, and 201 residues fro sea otter with stop codons located at analogous positions were identified. These fragments correspond to nucleotides 71–753 of the humanIL-6 transcript and represent 96% of the complete coding nucleotides. Comparison of these marine mammal sequences with other published mammalianIL-6 cDNA demonstrated that both harbor seal and sea otterIL-6 had most similarity to that of other terrestrial carnivores (Mustelidae and Canidae), while killer whale had highest identity with ruminants (Bovidae and Ovidae). Among the three marine mammal species characterized, as well as cDNA sequences from nine other species, 40 invariant amino acids, including a number of residues situated at the putative gp80 and gp130 receptor binding sites, were identified. The presence of invariant amino acids within the receptor-binding portion of IL-6 for twelve different species suggests these positions are essential for biological activity of IL-6 and, moreover, likely account for the cross-reactivity among different mammalian IL-6-like activities in mouse bioassays. An additional significant finding was the presence of several variant residues only within the mouse putative IL-6 receptor binding region, which may account for observations of restricted cross-reactivity of mouse IL-6-like activity in human bioassays. Together, these findings provide insights into the evolution of the mammalianIL-6 gene and additional valuable information regarding amino acid residues essential for the biological activity of mammalian IL-6.
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The nucleotide sequence data reported in this paper have been submitted to GSDB and have been assigned the accession numbers L46802 (harbor sealIL-6), L46803 (killer whaleIL-6), and L46804 (Southern sea otterIL-6)
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King, D.P., Schrenzel, M.D., McKnight, M.L. et al. Molecular cloning and sequencing of interleukin 6 cDNA fragments from the harbor seal (Phoca vitulina), killer whale (Orcinus orca), and Southern sea otter (Enhydra lutris nereis). Immunogenetics 43, 190–195 (1996). https://doi.org/10.1007/BF00587299
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DOI: https://doi.org/10.1007/BF00587299