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Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele

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Abstract

Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1 * 1401 (DRw14-Dw9) and DRB1 * 1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) was amplified enzymatically and sequenced after cloning intto a plasmid vector. The amino acid sequence of the first domain in the DRβ1 chain encoded in the DRB1-14c allele was more similar to that of the DRB1 * 1401 allele (three amino acid substitutions).than to that of the DRB1 * 1402 allele (six amino acid substitutions). No polymorphic amino acid residue that could explain the common serologic HLA-DRw14 specificity was identified among the sequences of the three DRw14-related alleles. Sequence-specific oligonucleotides (SSOs) were synthesized on the basis of the DRB1-14c nucleotide sequence and used for genotyping of the Japanese population. These SSOs served as useful probes for identifying the DRB1-14c allele in a wide range of donors.

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Author notes

  1. Akio Abe

    Present address: The Second Laboratory of Bacteriology, The Kitasato Institute, 5-9-1 Shirogane, Minato-ku, 108, Tokyo, Japan

Authors and Affiliations

  1. Department of Transplantation Immunology, Kitasato University School of Medicine, 1-15-1 Kitasato, 228, Sagamihara, Japan

    Fumiya Obata, Ichiro Ito, Koichi Ito, Akio Abe, Midori Ohkubo, Keiji Onda, Yong-guang Yang, Koji Watanabe & Noboru Kashiwagi

Authors
  1. Fumiya Obata
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  2. Ichiro Ito
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  3. Koichi Ito
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  4. Akio Abe
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  5. Midori Ohkubo
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  6. Keiji Onda
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  7. Yong-guang Yang
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  8. Koji Watanabe
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  9. Noboru Kashiwagi
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M33693.

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Obata, F., Ito, I., Ito, K. et al. Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele. Immunogenetics 32, 313–320 (1990). https://doi.org/10.1007/BF00211645

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  • DOI: https://doi.org/10.1007/BF00211645

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