Abstract
We previously reported that some rabbits have three different copies of T-cell receptor b (Tcrb) constant region genes unlike man, mice, and rats who generally have two copies. Two of theseCß genes were found on an ≈ 14 kilobase (kb) and one on an ≈ 6 kbEco RI fragment. The gene on the 6 kb fragment is of ß2 type. A previously described portion of the 14 kb fragment appeared to have sequences characteristic ofCß1. We have now shown that the 6 kb fragment is adjacent to and 3′ of the 14 kb fragment. Furthermore, the second linked sequence ofCß gene present on the 14 kb fragment resembles to a large extent theCß2 gene present on the 6 kb fragment. Moreover, this secondCß gene has a 5′ cluster ofJß sequences resemblingJß2 of other species. However, exon 4 and the 3′ unranslated region (3′UT) are of the ß1 type. Mapping studies using southern analyses of both genomic DNA and the 14 kb clone have identified another cluster ofJß2 sequences 5′ of the third tandemCß2 gene present on the 6 kbEco RI fragment. Thus, the second gene on the 14 kb fragment appears to be a chimeric genomicTcrb gene that may have arisen by an unequal crossing-over event analogous to that which may have deletedCß1,Dß2, andJß2 in NZW mice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Angelini, G., De Preval, C., Gorski, J., and Mach, B.: High resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes.Proc Natl Acad Sci USA 83: 4489–4493, 1986
Angiolillo, A.L., Lamoyi, E., Bernstein, K.E., and Mage, R.G.: Identification of genes for the constant region of rabbit T-cell receptor ß chains.Proc Natl Acad Sci USA 82: 4498–4502, 1985
Chien, Y.-H., Becker, D., Lindsten, T., Okamura, M., Cohen, D.I., and Davis, M.: A third type of murine T-cell receptor gene.Nature 312: 31–35, 1984a
Chien, Y.-H., Gascoigne, N.R.J., Kavaler, J., Lee, N.E., and Davis, M.M.: Somatic recombination in a murine T-cell receptor gene.Nature 309: 322–326, 1984b
Davis, M.M. and Bjorkman, P.J.: T-cell antigen receptor genes and T-cell recognition.Nature 334: 395–402, 1988
Duby, A.D., Klein, K.A., Murre, C., and Seidman, J.G.: A novel mechanism of somatic rearrangement predicted by a human T-cell antigen receptor β-chain complementary DNA.Science 228: 1204–1206, 1985
Feinberg, A.P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal Biochem 132: 6–13, 1983
Gascoigne, N.R.J., Chein, Y.-H., Becker, D.M., Kavaler, J., and Davis, M.M.: Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genes.Nature 310: 387–391, 1984
Harindranath, N., Lamoyi, E., and Mage, R.G.: Allotypes of the comstant region of the rabbit T cell receptor β chains.J Immunol 142: 3292–3297, 1989
Hedrick, S.M., Nielsen, E.A., Kavaler, J., Cohen, D.I., and Davis, M.M.: Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins.Nature 308: 153–158, 1984
Kenter, A.L. and Birshtein, B.K.: Chi, a promoter of generalized recombination in λ phage, is present in immunoglobulin genes.Nature 293: 402–404, 1981
Komatsu, M., Lamoyi, E., and Mage, R. G.: Genomic DNA encoding rabbit T-cell receptor beta chains: isotypes and allotypes of CP.J Immunol 138: 1621–1626, 1987
Kotzin, B.L., Barr, V.L., and Palmer, E.: A large deletion within the T-cell receptor beta-chain gene complex in New Zealand White mice.Science 229: 167–171, 1985
Lamoyi, E., Angiolillo, A.L., and Mage, R.G.: An evolutionarily conserved rabbit T-cell receptor β-chain variable region.Immunogenetics 23: 266–270, 1986
Mage, R.G.: Molecular biology of rabbit immunoglobulin and T-cell receptor genes.In S. Dubiski (ed.):The Rabbit in Contemporary Immunological Research, pp. 106–147, Longman Scientific and Technical, Harlow, England, 1987
Malissen, M., Minard, K., Mjolsness, S., Kronenberg, M., Goverman, J., Hunkapiller, T., Prystowsky, M.B., Yoshikai, Y., Fitch, F., Mak, T.W., and Hood, L.: Mouse T cell antigen receptor: structure and organization of constant and joining gene segment encoding the β polypeptide.Cell 37: 1101–1110, 1984
Maniatis, T., Fritsch, E.F., and Sambrook, J.:Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Marche, P.N. and Kindt, T.J.: Genes encoding the beta chain of the T cell antigen receptor in the rabbit.Adv Gene Technology 2: 243–244, 1985
Marche, P.N. and Kindt, T.J.: A variable region gene subfamily encoding T cell receptor β chains is selectively conserved among mammals.J Immunol 137: 1729–1734, 1986
Messing, J.: New M13 vectors for cloning.Methods Enzymol 101: 20–78, 1983
Nicolas, A., Treco, D., Schultes, N.P., and Szostak, J..: An initiation site for meiotic gene conversion in the yeast Saccharomyces cerevisiae.Nature 338: 35–39, 1989
Noonan, D.J., Kofler, R., Singer, P.A., Cardenas, G., Dixon, F.J., and Theofilopoulos, A.N.: Delineation of a defect in T-cell receptor β genes of NZW mice predisposed to autoimmunity.J Exp Med 163: 644–653, 1986
Sanger, F., Nicklen, S., and Coulson, A.R.: DNA sequencing with chain terminating inhibitors.Proc Natl Acad Sci USA 74: 5463–5467, 1977
Shapiro, M.B. and Senapathy, P.: Automated preparation of DNA sequences for publication.Nucleic Acids Res 14: 65–73, 1986
Sim, G.K., Yague, J., Nelson, J., Marrack, P., Palmer, E., Augustin, A., and Kappler, J.: Primary structure of human T-cell receptor α-chain.Nature 312: 771–775, 1984
Sims, J.E., Tunnacliffe, A., Smith, W.J., and Rabbits, T.H.: Complexity of human T-cell antigen receptor β-chain constant- and variableregion genes.Nature 312: 541–545, 1984
Sun, H., Treco, D., Schultes, N.P., and Szostak, J.W.: Double strand breaks at an initiation site for meiotic gene conversion.Nature 38: 87–90, 1989
Toyonaga, B., Yoshikai, Y., Vadasz, V., Chin, B., and Mak, T.W.: Organization and sequences of the diversity, joining and constant region genes of the human T-cell receptor β chain.Proc Natl Acad Sci USA 82: 8624–8628, 1985
Tunnacliffe, A. and Rabbitts, T.H.: Sequence of the Dβ2-Jβ2 region of the human T-cell receptor β-chain locus.Nucleic Acids Res 13: 6651–6661, 1985
Tunnacliffe, A., Kefford, R., Milstein, C., Forster, A., and Rabbitts, T.H.: Sequence and evolution of the human T-cell antigen receptor β chain genes.Proc Natl Acad Sci USA 82: 5068–5072, 1985
Wu, S., Saunders, T.L., and Bach, F.H.: Polymorphism of human la antigens generated by reciprocal intergenic exchange between two DRβ loci.Nature 324: 676–679, 1986
Yanagi, Y., Yoshikai, Y., Leggett, K., Clark, S.P., Aleksander, I., and Mak, T.W.: A human T-cell specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains.Nature 308: 145–149, 1984
Yanisch-Perron, C., Vieira, J., and Messing, J.: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13 mp18 and pUC19 vectors.Gene 33: 103–119, 1985
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned accession number M26603.
Rights and permissions
About this article
Cite this article
Harindranath, N., Lamoyi, E. & Mage, R.G. Genomic sequence and organization of rabbitTcrb constant region genes. Immunogenetics 30, 465–474 (1989). https://doi.org/10.1007/BF02421179
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02421179