Abstract
TheQ genes, specifying Qa antigens and situated in the extended part of the major histocompatibility complex (MHC) of the mouse, comprise a subgroup of MHC class I genes whose significance and function are still largely unknown. In screening a cDNA library made from the BALB/c inducer T-cell line Cl.Ly1-T1, we isolated 11 clones representingQ8/9, but none representingQ6 orQ7. Confirmatory evidence is given that theQ8/9 gene originated from fusion of the 5′ region of theQ8 gene with the 3′ region of theQ9 gene at a recombination site or hot spot in the vicinity of intron 4. Contrary to previous impressions thatQ8/9 is an inert pseudogene, we find that theQ8/9 gene can be functional and encode a Qa-2,3 antigen. One variety of the 11 Q8/9 clones isolated lacked exon 5, which encodes the transmembrane domain of class I glycoproteins, and thus may account for secretion of a soluble form of Qa-2,3 antigen thought to be released by activated T cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boyse, E. A.: The biology of Tla.Cell 38: 1–2, 1984
Chen, Y. T., Obata, Y., Stockert, E., and Old, L. J.: Thymus-leukemia (TL) antigens of the mouse. Analysis of TL mRNA and TL cDNA from TL+ and TL− strains.J. Exp Med 162: 1134–1148, 1985
Devlin, J., Weiss, E. H., Paulson, M., and Flavell, R. A.: Duplicated gene pairs and alleles of class I genes in the Qa2 region of the murine major histocompatibility complex: a comparison.EMBO J 4: 3203–3207, 1985
Eastman O'Neill, A., Reid, K., Garberim, J. C., Karl, M., and Flaherty, L.: Extensive deletions in theQ region of the mouse major histocompatibility complex.Immunogenetics 24: 368–373, 1986
Fahrner, K., Hogan, B. L. M., and Flavell, R. A., Transcription of H-2 and Qa genes in embryonic and adult mice.EMBO J 6: 1265–1271, 1987
Flaherty, L.: The Tla region of the mouse: identification of a new serologically defined locus, Qa-2.Immunogenetics 3: 533–539, 1976
Flaherty, L: Some recent data on the Q-subregion genes and their products.In C. David (ed.):H-2 Antigens: Genes, Molecules, Function, pp. 133–138 NATO ASI Series, Plenum Press, 1987
Flaherty, L., DiBiase, K., Lynes, M. A., Seidman, J. G., Weinberger, O., and Rinchik, E. M.: Characterization of a Q subregion in the murine major histocompatibility complex.Proc Natl Acad Sci USA 82: 1503–1507, 1985
Maniatis, T., Fritsch, E. F., and Sambrook, J.:Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, 1982
Matsuura, A., Shen, F. W., Fisher, D. A., Hood, L. and Boyse, E. A.: Transcripts ofTla genes.Immunogenetics 25: 411–415, 1987
Mellor, A. L., Weiss, E. H., Kress, M., Jay, G., and Flavell, R. A.: A nonpolymorphic class I gene in the murine major histocompatibility complex.Cell 36: 139–144, 1984
Mellor, A. L., Antoniou, J., and Robinson, P.: Structure and expression of genes encoding murine Qa-2 class I antigens.Proc Natl Acad Sci USA 82: 5920–5924, 1985
Okayama, H. and Berg, P.: A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.Mol Cell Biol 3: 280–289, 1983
Robinson, P. J.: Two different biosynthetic pathways for the secretion of Qa region-associated class I antigens by mouse lymphocytes.Proc Natl Acad Sci USA 84: 527–531, 1987
Robinson, P. J., Bevec, D., Mellor, A. L., and Weiss, E. H.: Sequence of the mouseQ4 class I gene and characterization of the gene product.Immunogenetics 27: 79–86, 1988
Rothenberg, E. and Boyse, E. A.: Synthesis and processing of molecules bearing thymus leukemia antigen.J. Exp Med 150: 777–791, 1979
Sanger, F., Nicklen, S., and Coulson, A. R.: DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci USA 74: 5463–5467, 1977
Soloski, M. J., Vernachio, J., Einhorn, G., and Lattimore, A.: Qa gene expression: biosynthesis and secretion of Qa-2 molecules in activated T cells.Proc Natl Acad Sci USA 83: 2949–2953, 1986
Soloski, M. J., Hood, L., and Stroynowski, I.: Qa-region class I gene expression: identification of a second class I gene, Q9, encoding a Qa-2 polypeptide.Proc Natl Acad Sci USA 85: 3100–3104, 1988
Stanton, T. H. and Boyse, E. A.: A new serologically defined locus in theTla region of the mouse,Qa-1. Immunogenetics 3: 525–531, 1976
Steinmetz, M., Frelinger, J. G., Fisher, D., Hunkapiller, T., Pereira, D., Weissman, S. M., Uehara, H., Nathenson, S., and Hood, L.: Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.Cell 24: 125–134, 1981a
Steinmetz, M., Moore, K. W., Frelinger, J. G., Taylor Sher, B., Shen, F. W., Boyse, E. A., and Hood, L.: A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains.Cell 25: 683–692, 1981b
Steinmetz, M., Winoto, A., Minard, K., and Hood, L.: Clusters of genes encoding mouse transplantation antigens.Cell 28: 489–498, 1982
Stiernberg, J., Low, M. G., Flaherty, L., and Kincade, P. W.: Removal of lymphocyte surface molecules with phosphatidylinositol-specific phospholipase C: effects on mitogen responses and evidence that ThB and certain Qa antigens are membrane-anchored via phosphatidylinositol,J Immunol 38: 3877–3884, 1987
Stroynowski, I., Soloski, M., Low, M. G., and Hood, L.: A single gene encodes soluble and membrane-bound forms of the major histocompatibility Qa-2 antigen: anchoring of the product by a phospholipid tail.Cell 50: 759–768, 1987
Waneck, G. L., Sherman, D. H., Calvin, S., Allen, H., and Flavell, R. A.: Tissue-specific expression of cell-surface Qa-2 antigen from a transfected Q7b gene of C57BL/10 mice.J Exp Med 165: 1358–1370, 1987
Waneck, G. L., Sherman, D. H., Kincade, P. W., Low, M. G., and Flavell, R. A.: Molecular mapping of signals in Qa-2 required for attachment of the phosphatidylinositol membrane anchor.Proc Natl Acad Sci USA 85: 577–581, 1988
Wigler, M. A., Pellicer, A., Silverstein, S., Axel, R., Urlaub, G., and Chasin, L.: DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.Proc Natl Acad Sci USA 76: 1373–1376, 1979
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsuura, A., Schloss, R., Shen, FW. et al. Expression of theQ8/9 d gene by T cells of the mouse. Immunogenetics 30, 156–161 (1989). https://doi.org/10.1007/BF02421200
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02421200