Abstract
Male-specific antigen expression by XY teratocarcinomas PCC7 and 7′ is demonstrated first by the rejection of tumors by female but not by male mice following challenge with these cell lines. Male-specific antigen expression is confirmed by an indirect method in which females are immunized against H-Y antigen by male skin grafts. A variant of PCC7 lacking male-specific antigen expression is described. Analysis of the karyotype and of the DNA from this variant indicate that the loss of male-specific antigen expression is a result of the loss of the Y chromosome. The ability to recover variants that have lost expression of male-specific antigen opens the possibility of their selection after mutagenesis.
Similar content being viewed by others
References
Avner, P. R., Dove, W. F., Dubois, P., Gaillard, J. A., Guenet, J. L., Jacob, F., Jakob, H., and Shedlovsky, A.: The genetics of teratocarcinoma transplantation: Tumor formation in allogeneic hosts by the embryonal carcinoma lines F9 and PCC3. Immunogenetics 7: 103–115, 1978
Bailey, D. W.: Allelic forms of a gene controlling the female immune response to the male antigen in mice. Transplantation 11: 426–428, 1971
Bailey, D. W.: Genetics of histocompatibility in mice. I. New loci and congenic lines. Immunogenetics 2: 249–256, 1975
Bailey, D. W. and Hoste, J.: A gene governing the female immune response to the male antigen in mice. Transplantation 11: 404–407, 1971
Bailey, D. W. and Usama, B.: A rapid method of grafting skin on tails of mice. Transplant. Bull. 7: 424–425, 1960
Bunker, M. C.: Y-chromosome loss in transplanted testicular teratomas in mice. Can. J. Genet. Cytol. 8: 312–327, 1966
Casanova-Bettane, M., Latron, F., Jakob, H., and Fellous, M.: A quantitative radioimmunoassay for membranous and soluble H-Y antigen typing. Hum. Genet. 58: 21–24, 1981
Carroll, P. G. and DeWolf, W. C.: Immune properties of human teratocarcinoma. I. Human teratocarcinoma targets distinguish between natural killer and activated killer cells. J. Immunol. 131: 1007–1010, 1983
Eicher, E. M. and Washburn, L. L.: Assignment of genes to regions of mouse chromosomes. Proc. Natl. Acad. Sci. U.S.A. 75: 946–950, 1978
Eicher, E. M., Washburn, L. L., Whitney, J. B., III, and Morrow, K. E.: Mus poschiavinus Y chromosome in the C57BL/6J murine genome causes sex reversal. Science 217: 535–537, 1982
Eichwald, E. and Silmser, C.R.: Communication. Transplant. Bull. 2: 148–149, 1955
Fellous, M., Günther, E., Kemler, R., Wiels, J., Berger, R., Guenet, J. L., Jakob, H., and Jacob, F.: Association of H-Y male antigen with β2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines. J. Exp. Med. 148: 58–70, 1978
Gasser, D. L. and Silvers, W. K.: Genetic control of the immune response in mice. III. An association between H-2 type and reaction to H-Y. J. Immunol. 106: 875–876, 1971
Goldberg, E. H., Boyse, E. A., Bennett, D., Scheid, M., and Carswell, L. A.: Serological demonstration of H-Y (male) antigen on mouse sperm. Nature 232: 478–480, 1971
Gordon, R., Simpson, E., and Samelson, L.: In vitro cell mediated immune response to the H-Y antigen. J. Exp. Med. 142: 1108–1120, 1975
Jacob, F.: Mouse teratocarcinoma and embryonic antigens. Immunol. Rev. 33: 1–32, 1977
Johnson, L. L., Clipson, L. J., Dove, W. F., Feilbach, J.,Maher, L. J., and Shedlovsky, A.: Teratocarcinoma antigens are encoded in the H-2 region. Immunogenetics 18: 137–145, 1983
Johnson, L. L., Sargent, E. L., Washburn, L. L., and Eicher, E. M.: XY female mice express H-Y antigen. Der. Genet. 3: 247–253, 1982
Koo, G. C., Reidy, J. A., and Negamine, C. M.: H-Y antigen in XO mice. Immunogenetics 18: 37–44, 1983
Maher III, L. J. and Dove, W. F.: Overt expression of H-2 serotypes on EC cells is not necessary for host rejection. Immunogenetics, in press, 1984
McBurney, M. W. and Rogers, B. J.: Isolation of male embryonal carcinoma cells and their chromosomal replication patterns. Dev. Biol. 89: 503–508, 1982
Mintz, B. and Illmensee, K.: Normal genetically mosaic mice produced from malignant teratocarcinoma cells. Proc. Natl. Acad. Sci. U.S.A. 72: 3585–3589, 1975
Morello, D., Daniel, F., Baldacci, P., Cayre, Y., Gachelin, G., and Kourilsky, P.: Absence of significant H2 and β2-microglobulin mRNA expression by mouse embryonal carcinoma cells. Nature 296: 264–262, 1982
Ostrand-Rosenberg, S., Cohn, A. L., Sandoz, J. W., Schwartzman, M. L., and McCarthy, C.: Host regulation of major histocompatibility complex antigens on 402AX teratocarcinoma cells during tumor rejection. In L. Silver, G. Martin, and S. Strickland (eds.): Teratocarcuromu Stem Cells, pp. 519–529, Cold Spring Harbor Laboratory, Cold Spring Harbor. New York, 1983
Pfeiffer, S. E., Jakob, H., Mikoshiba, K., Dubois, P., Guénet, J. L., Nicholas, J.-F., Gaillard, J., Chevance, G., and Jacob, F.: Differentiation of a teratocarcinoma line: preferential development of cholinergic neurons. J. Cell Biol. 88: 57–66, 1981
Phillips, S. J., Birkenmeier, E. H., Callahan, R., and Eicher, E. M.: Male and female mouse DNAs can be discriminated using retroviral probes. Nature 297: 241–243, 1982
Rigby, P. W., Dieckmann, M., Rhodes, C., and Berg, P.: Labelling deoxyribonucleic acids to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113: 237–152, 1977
Schedl, T. and Dove, W. F.: Mendelian analysis of the organization of actin sequences in Physarum polycephalum. J. Mol. Biol. 160: 41–57, 1982
Shedlovsky, A., Clipson, L. J., VandeBerg, J. L., and Dove, W. F.: Strong teratocarcinoma transplantation loci, Gt-1 and Gt-2, flank H-2. Immunogenetics 13: 413–419, 1981
Silvers, W. K., Gasser, D. L., and Eicher, E. M.: H-Y antigen, serologically detectable male antigen, and sex determination. Cell 28: 439–440, 1982
Simpson, E. and Gordon, R. D.: Responsiveness to H-Y antigen: Ir gene complementation and target cell specificity. Immunol. Rev. 35: 59–75, 1977
Southern, E.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mal. Biol. 98: 503–517, 1975
Stern, P., Gidlund, M., Orn, A., and Wigzell, H.: Natural killer cells mediate lysis of embryonal carcinoma cells lacking the MHC. Nature 285: 341–342, 1980
Stevens, L. C.: The biology of teratomas. In M. Abercrombie and J. Brachet (eds.): Advances in Morphogenesis, pp. 1–31, Academic Press, New York, 1967
Stewart, T. A. and Mintz, B.: Successive generations of mice produced from an established line of euploid teratocarcinoma cells. Proc. Natl. Acad. Sci. U.S.A. 78: 6314–6318, 1981
Wahl, G. M., Stern, M., and Stark, G. R.: Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization using dextran sulfate. Proc. Natl. Acad. Sci. U.S.A. 76: 3683–3687, 1979
Author information
Authors and Affiliations
Additional information
Aspects of this work have been presented at the 10th Cold Spring Harbor Conference on Cell Proliferation, September 1982
Rights and permissions
About this article
Cite this article
Johnson, L.L., Dove, W.F. Male-specific transplantation antigen expression by XY teratocarcinomas PCC7 and 7′. Immunogenetics 19, 233–241 (1984). https://doi.org/10.1007/BF00364766
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00364766