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A new mouse cell-surface antigen (Ly-m20) controlled by a gene linked to Mls locus and defined by monoclonal antibodies

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Abstract

Five monoclonal antibodies were established by the fusion of mouse myeloma cells (NS.1) with spleen cells from A and (A x C3H/An)F1 mice hyperimmunized with 70Z/3 tumor cells. These antibodies recognized a new antigenic specificity provisionally called Ly-m20.2. In direct cytotoxicity assays, 60 percent of cells in spleen, 40 percent in lymph node, 50 percent in bone marrow and less than 5 percent in thymus were found to react with three of the five antibodies, whereas the two others yielded somewhat lower cytotoxicity indices. The Ly-m20.2 antigen was also expressed on cells derived from liver and kidney but not on cells derived from brain. As judged from cytotoxicity assays with separated T and B cells, Ly-m20.2 antigen is carried preferentially on B lymphocytes. Direct plaque-forming cells (PFC) were completely eliminated by Ly-m20.2-specific antibody and complement. Linkage tests by analysis in 20 (CBA/J x C3H/An) x C3H/An backcross mice and by segregation analysis of BXH and SWXL recombinant inbred strains indicate close association of the loci controlling Ly-m20.2 and Mls antigens on chromosome 1.

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Abbreviations

MLR:

mixed lymphocyte reaction

MHC:

major histocompatibility complex

Mls:

minor lymphocyte-stimulating antigen

Ia:

I-region associated

PFC:

plaque-forming cell

SRBC:

sheep red blood cell

Con A:

concanavalin A

LPS:

lipopolysaccharide

GVH:

graft-versus-host

HVG:

host-versus-graft

CML:

cell-mediated lympholysis

LD:

lymph ocyte-defined

SD:

serologically defined

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Authors and Affiliations

  1. Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, New York

    Shoji Kimura, Nobuhiko Tada, Eiichi Nakayama, Yen Liu & Ulrich Hämmerling

Authors
  1. Shoji Kimura
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  2. Nobuhiko Tada
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  3. Eiichi Nakayama
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  4. Yen Liu
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  5. Ulrich Hämmerling
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Additional information

The prefix m (monoclonal) is used following a suggestion by Klein and co-workers (1979).

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Kimura, S., Tada, N., Nakayama, E. et al. A new mouse cell-surface antigen (Ly-m20) controlled by a gene linked to Mls locus and defined by monoclonal antibodies. Immunogenetics 14, 3–14 (1981). https://doi.org/10.1007/BF00344295

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  • DOI: https://doi.org/10.1007/BF00344295

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