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A new mouse cell-surface antigen (Ly-m18) defined by a monoclonal antibody

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Abstract

Spleen cells from C3H/An mice immunized with spleen cells of C57BL/6-H-2 k mice were fused with myeloma cell line NS.1. One established hybrid cell line continuously secreted antibody that recognized a new surface antigen provisionally called Ly-m18. The new alloantigen is expressed on 90 percent of thymus cells, 55 percent of spleen cells, and 45 percent of either lymphnode or bone-marrow cells. It is also expressed on cells derived from brain, kidney, and liver. Fifty percent of either peripheral T or B cells express the Ly-m18 antigen, and some tumor cell lines with T, B, pre-B or stem cell characteristics are Ly-m18 (+). The strain distribution pattern distinguishes Ly-m1 8 antigen from all other murine lymphocyte alloantigens. The typing data of two sets of CXB and AKXL recombinant inbred strains indicate that the Ly-m18 gene is linked to the Ltw-2 locus which has not yet been assigned to a chromosome.

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Abbreviations

RI:

recombinant inbred

Con-A:

concanavalin A

LPS:

lipopolysaccharide

MLR:

mixed lymphocyte reaction

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Authors and Affiliations

  1. Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, 10021, New York, New York

    Shoji Kimura, Nobuhiko Tada, Yen Liu & Ulrich Hämmerling

Authors
  1. Shoji Kimura
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  2. Nobuhiko Tada
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  3. Yen Liu
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  4. Ulrich Hämmerling
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Additional information

The prefix m (monoclonal) is used following a suggestion by Klein and co-workers (1979).

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Kimura, S., Tada, N., Liu, Y. et al. A new mouse cell-surface antigen (Ly-m18) defined by a monoclonal antibody. Immunogenetics 13, 547–554 (1981). https://doi.org/10.1007/BF00343722

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  • DOI: https://doi.org/10.1007/BF00343722

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