Abstract
The anti-H-2 alloantiserum D-32 [(BlO.A(2R) × C3H.SW) anti-C3H] is cytolytic to human lymphocytes. Fab2 blocking assays, indirect immunoprecipitation and sequential immunoprecipitation experiments showed that the anti-H-2 alloantiserum D-32 recognizes antigenic determinants which are expressed on the heavy chain of subpopulations of HLA-A, B antigens. These determinants are different from those defining the serological polymorphism of the HLA-A, B, C system, are the same as or spatially close to those recognized by the anti-HLA-A, B monoclonal antibody Q6/64 and are expressed on rabbit, rat or guinea pig lymphocytes.
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Allison, J. P., Belvedere, M., Reisfeld, R. A., Pellegrino, M. A., and Ferrone, S.: Serological and immunochemical characterization of HLA-A9 xenoantisera. J. Immunol. 121: 579–585, 1978
Bonner, W. M. and Laskey, R. A.: A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46: 83–88, 1974
Evrin, P. E. and Nilsson, K.: β2-microglobulin production in vitro by human hematopoietic, mesenchymal and epithelial cells. J. Immunol. 112: 137–144, 1974
Ey, P. L., Prowse, S. J., and Jenkin, C. R.: Isolation of pure IgGI, IgG2 a and IgG2 b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry 15: 429–436, 1978
Ferrone, S., Ting, A., Pellegrino, M. A., Terasaki, P.I., and Reisfeld, R. A.: Utilization of cultured human lymphoid cells for detection of humoral sensitization in prospective recipients of kidney transplants. J. Clin. Invest. 55: 388–394, 1975
Ferrone, S., Pellegrino, M. A., and Mittal, K. K.: Natural antibodies to human lymphocytes in murine strains. J. Nad. Cancer Inst. 56: 75–78, 1976
Ferrone, S., Belvedere, M., and Pellegrino, M. A.: Structural relationship between W4/W6 antigens and other surface markers on cultured human lymphoid cells as determined by the “lysostrip” method. Immunogenetics 6: 161–169, 1978
Götze, D., Lee, S., Ferrone, S., Pellegrino, M. A., and Reisfeld, R. A.: Cross-reactivity between HL-A and H-2 antigens. II. Allogeneic sensitization by xenogeneic soluble lymphocyte antigens (HLA). Transplant. Proc. 5: 467–470, 1973
Iványi, P., van den Berg-Loonen, E., and de Greeve, P.: Anti-H-2Dd antibodies cross-react with HLA-All and Aw31. Tissue Antigens 11: 439–442, 1978
Ivašková, E., Dausset, J., and Iványi, P.: Cytotoxic reactions of anti-H-2 sera with human lymphocytes. Folia Biol. (Praha) 18: 194–197, 1972
Ivašková, E. and Iványi, P.: Cytotoxic effect of anti-H-2 sera on human lymphocytes: Association with A7 and W27. Folia Biol. (Praha) 20: 283–285, 1974
Ivašková, E., Iványi, P., Bavorová, H., and Egorov, I. K.: Cross-reactions of some anti-H-2p sera with HL-A9. Folia Biol. (Praha) 22: 58–59, 1976
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970
McMaster, W. R., Winearls, B. C., and Parham, P.: A monoclonal mouse anti-rat la antibody which cross reacts with a human HLA-DRw determinant. Tissue Antigens 14: 453–458, 1979
Nisonoff, A., Wissler, F. C., and Woernly, D. L.: Properties of univalent fragments of rabbit antibody isolated by specific adsorption. Arch. Biochem. Biophys. 88: 241–249, 1960
Parham, P., Barnstable, C. J., and Bodmer, W. F.: Use of a monoclonal antibody (W6/32) in structural studies of HLA-A, B, C antigens. J. Immunol. 123: 342–349, 1979
Pellegrino, M. A., Ferrone, S., Mittal, K. K., Götze, D., Terasaki, P. I., and Reisfeld, R. A.: Cross-reactivity between human and murine lymphocyte antigens III. Reactivity of H-2 allo- and xenoantisera with human lymphoid cells. Immunogenetics 1: 158–173, 1974
Pellegrino, M. A., Ferrone, S., Dierich, M. P., and Reisfeld, R. A.: Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydryl compound 2-amino ethylisothiouronium bromide. Clin. Immunol. Immunopath. 3: 324–333, 1975
Quaranta, V., Walker, L. E., Pellegrino, M. A., and Ferrone, S.: Purification of immunologically functional subsets of human Ia-like antigens on a monoclonal antibody (Q5/13) immunoadsorbent. J. Immunol. 125: 1421–1425, 1980a
Quaranta, V., Imai, K., Molinaro, G. A., and Ferrone, S.: Manufacture of monoclonal antibodies to human melanoma associated and histocompatibility antigens. In R. M. Nakamura, W. R. Dito, and E. S. Tucker III (eds.): Immunoassays: Clinical Laboratory Techniques for the 1980 s, pp. 301–324, A. R. liss, Inc., New York, 1980b
Reisfeld, R. A., Sevier, E. D., Pellegrino, M. A., Ferrone, S., and Poulik, M. D.: Association of HL-A antigens and β2-microglobulin at the cellular and molecular level. Immunogenetics 2: 183–197, 1975
Smilek, D. E., Boyd, H. C., Wilson, D. B., Zmijewski, C. M., Fitch, F. W., and McKearn, T. J.: Monoclonal rat anti-major histocompatibility complex antibodies display specificity for rat, mouse and human target cells. J. Exp. Med. 151: 1139–1150, 1980
Uehara, H., Ewenstein, B. M., Martinko, J. M., Nathenson, S. G., Coligan, J. E., and Kindt, T. J.: Primary structure of murine major histocompatibility complex alloantigens: Amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein. Biochemistry 19: 306–315, 1980
Waxdal, M. J., Basham, T. Y., Clement, L. T., Shevach, E. M., and Schwartz, B. D.: Amino terminal sequence studies on the la antigens of the guinea pig. Mol. Immunol. 16: 61–65, 1979
Wilson, B. S., Indiveri, F., Pellegrino, M. A., and Ferrone, S.: Production and characterization of DR xenoantisera: Use for detection of serum DR antigens. J. Immunol. 122: 1967–1971, 1979
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Quaranta, V., Pellegrino, M.A., Callahan, G.N. et al. Cross-reactivity between human and murine lymphocyte antigens. Immunogenetics 13, 311–317 (1981). https://doi.org/10.1007/BF00364496
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DOI: https://doi.org/10.1007/BF00364496