Abstract
In order to investigate linkage, we used serum allotypes of the two rabbit C κ isotypes and restriction fragment length polymorphisms (RFLPs) of the genes for V κ, C κ, and T-cell receptor C β . The inheritance of these genetic markers was studied through backcross and F2 matings. Southern analysis and hybridization of genomic DNA with a Cκ probe detected a 5 kb Pst I fragment linked to expression of the K2bas1 allotype and the presence of the κ1b bas gene and a 6.6 kb Pst I fragment linked to the expression of the K1b9 allotype, the presence of the κ2 bas2 gene and lack of expression of the K2bas1 allotype. A Vκ probe detected a 1.3 kb Eco RI fragment linked to the presence of the κ1b bas gene and expression of the K2bas1 allotype. In contrast, the 9 or 14 kb Eco RI RFLP (C β a or C β b) detected with a Tcr β chain probe segregated independently from Cκ allotypes and RFLPs. It has previously been found that Cκ and C β are also unlinked in man, whereas in the mouse they are linked at a distance of ≈8 centimorgans.
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Hole, N.J.K., Lamoyi, E., Komatsu, M. et al. Linked genetic markers of the rabbit κ light chain are not linked to the Tcr β chain genes. Immunogenetics 28, 99–107 (1988). https://doi.org/10.1007/BF00346157
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DOI: https://doi.org/10.1007/BF00346157