Abstract
A two-component solution theory is studied which incorporates hydrophobic matching as a major contribution to the lipid-protein interactions in biological membranes. A special geometrical constraint has been discovered which has important implications for the quantitative interpretation of physical effects to lipid-protein interactions. The theory has an advantage over conventional Landau-type phenomenological descriptions in that it accounts for phase separation. A certain class of experimental systems, photosynthetic reaction centre and antenna proteins reconstituted into synthetic lipid membranes of different hydrophobic thicknesses, are considered with a view to determining the parameters of the theory. The theoretical predictions are found to be in good agreement with experimental measurements of shifts in the phase transition temperature.
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Abbreviations
- Dn c PC:
-
diacyl0glycero-PC with n acyl chain carbon atoms
- D12PC:
-
l-α-dilauroyl PC
- D13PC:
-
l-α-ditridecanoyl PC
- D14PC:
-
l-α-dimyristoyl PC
- D15PC:
-
l-α-dipentadecanoyl PC
- D16PC:
-
l-α-dipalmitoyl PC
- LHCP:
-
light harvesting chlorophyll protein
- NMR:
-
nuclear magnetic resonance
- PC:
-
phosphatidylcholine
- RC:
-
reaction centre
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Supported by the Danish Natural Science Research Council under grant J.nr. 5.21.99.72
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Sperotto, M.M., Mouritsen, O.G. Dependence of lipid membrane phase transition temperature on the mismatch of protein and lipid hydrophobic thickness. Eur Biophys J 16, 1–10 (1988). https://doi.org/10.1007/BF00255320
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DOI: https://doi.org/10.1007/BF00255320