Abstract
The translational diffusion of pyrene, pyrene butyric acid and pyrene decanoic acid has been determined in phosphatidylcholine bilayers of different chain length and under pressure up to 200 bars. In the liquid crystalline phase and at a given temperature the diffusion decreases with increasing chain length. At a constant reduced temperature, T red (about 10 K above the transition temperature), long chain lipids exhibit the fastest diffusion which is in disagreement with hydrodynamic models but favours free volume models for diffusion in lipid bilayers. The volume of activation, V act, calculated from the decrease of the diffusion coefficient with pressure, ∂ln D/∂P, depends on lipid chain length. V act decreases with decreasing lipid chain length at a given temperature, T=65°C, and increases at the reduced temperature. These results are again in agreement with the dependence of the diffusion on lipid chain length and therefore with the free volume model.
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Abbreviations
- DLPC:
-
Dilauroylphosphatidylcholine
- DMPC:
-
Dimyristoylphosphatidylcholine
- DPPC:
-
Dipalmitoylphosphatidylcholine
- DSPC:
-
Distearoylphosphatidylcholine
- LUV:
-
Large unilamellar vesicles
- SUV:
-
Small unilamellar vesicles
- Tris:
-
Tris(hydroxymethyl)aminomethan
References
Clegg RM, Vaz WLC (1985) Translational diffusion of proteins and lipids in artificial lipid bilayer membranes. A comparison of experiment with theory. In: Watts A, De Pont JJHHM (eds) Progress in protein-lipid interactions. Elsevier, Amsterdam, pp 173–229
Cohen MH, Turnbull D (1959) Molecular transport in liquids and glasses. J Chem Phys 31:1164–1169
Cornell BA, Separovicz F (1983) Membrane thickness and acyl chain length. Biochim Biophys Acta 733:189–193
Derzko Z, Jacobson K (1980) Comparative lateral diffusion of fluorescent lipid analogues in phospholipid multibilayers. Biochemistry 19:6050–6057
Galla HJ, Hartmann W (1980) Excimer-forming lipids in membrane research. Chem Phys Lipids 27:199–219
Galla HJ, Hartmann W (1981) Pyrene decanoic acid and pyrene lecithin. Methods Enzymol 72:471–479
Galla HJ, Sackmann E (1974a) Lateral diffusion in the hydrophobic region of membranes: Use of pyrene excimers as optical probes. Biochim Biophys Acta 339:103–115
Galla HJ, Sackmann E (1974b) Lateral mobility of pyrene in model membranes of phospholipids with different chain length. Ber Bunsenges Phys Chem 78:949–953
Galla HJ, Hartmann W, Theilen U, Sackmann E (1979) On two-dimensional passive random walk in lipid-bilayers and fluid channels of biomembranes. J Membr Biol 48: 215–236
Kapitza HG, Rüppel D, Galla HJ, Sackmann E (1984) Lateral diffusion of lipids and proteins in solid phosphatidylcholine bilayers: The role of structural defects. Biophys J 45:577–587
Luisetti J, Möhwald H, Galla HJ (1979) Monitoring the location profile of fluorophores in phosphatidylcholine bilayers by the use of paramagnetic quenching. Biochim Biophys Acta 552:519–530
McCall DW, Douglass DC, Anderson EW (1959) Self-diffusion in liquids: Paraffin hydrocarbons. Phys Fluids 2:87–91
Müller HJ, Galla HJ (1983) Pressure variation of the lateral diffusion in lipid bilayer membranes. Biochim Biophys Acta 733:291–294
Müller HJ, Luxnat M, Galla HJ (1986) Lateral diffusion of small solutes and partition of amphipaths in defect structures of lipid bilayers. Biochim Biophys Acta 856:283–289
Nagle JF, Wilkinson DA (1978) Lecithin bilayers. Density measurements and molecular interactions. Biophys J 23:159–175
Peters R, Beck K (1983) Translational diffusion in phospholipid monolayers measured by fluorescence microphotolysis. Proc Natl Acad Sci USA 80:7183–7187
Saffman PG (1976) Brownian motion in thin sheets of viscous fluid. J Fluid Mech 73:593–602
Saffman PG, Delbrück M (1975) Brownian motion in biological membranes. Proc Natl Acad Sci USA 72:3111–3113
Schmidt G, Knoll W (1985) Densitometric characterization of aqueous lipid dispersions. Ber Bunsenges Phys Chem 89:36–43
Szoka F Jr, Papahadjopoulos D (1978) Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Biochemistry 75:4194–4198
Szoka F Jr, Papahadjopoulos D (1980) Comparative properties and methods of preparation of lipid vesicles (liposomes). Annu Rev Biophys Bioeng 9:467–508
Vaz WLC, Hallmann D (1983) Experimental evidence against the applicability of the Saffmann-Delbrück model to the translational diffusion of lipids in phosphatidylcholine bilayer membranes. FEBS Lett 152:287–290
Vaz WLC, Criado M, Madeira VMC, Schoellmann G, Jovin TM (1982) Size dependence of the translational diffusion of large integral membrane proteins in liquid-crystalline phase lipid bilayers. A study using fluorescence recovery after photobleaching. Biochemistry 21:5608–5612
Vaz WLC, Clegg RM, Hallmann D (1985) Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory. Biochemistry 24:781–786
Vogel H, Weiss A (1981a) Transport properties of liquids. I. Self-diffusion, viscosity and density of nearly spherical and disk like molecules in the pure liquid phase. Ber Bunsenges Phys Chem 85:539–548
Vogel H, Weiss A (1981b) Transport properties of liquids. II. Self-diffusion of almost spherical molecules in athermal liquid mixtures. Ber Bunsenges Phys Chem 85:1022–1026
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Müller, HJ., Galla, HJ. Chain length and pressure dependence of lipid translational diffusion. Eur Biophys J 14, 485–491 (1987). https://doi.org/10.1007/BF00293258
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DOI: https://doi.org/10.1007/BF00293258