Abstract
The inhibition of serum cholinesterase by metoclopramide has been previously characterised in vitro at high dilution of the enzyme. We examined the effect of varying enzyme dilution over a range of 1000 fold dilution, and assay temperature at 25°C and 37°C on the fractional inhibition of enzyme activity by metoclopramide. Neither enzyme concentration nor reaction temperature affected this fractional inhibition. Concentrations of metoclopramide producing 50% inhibition of enzyme activity were in the range 1.0–1.9×10−6 M. Lineweaver-Burk analysis of the enzyme reaction suggests that the pattern of this inhibition is competitive.
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Graham, S.G., Crossley, A.W.A. The characteristics of the inhibition of serum cholinesterase by metoclopramide. Eur J Clin Pharmacol 48, 225–228 (1995). https://doi.org/10.1007/BF00198302
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DOI: https://doi.org/10.1007/BF00198302