Qualitative and quantitative analysis of carbohydrates in green juices (wild mix grass and alfalfa) from a green biorefinery by gas chromatography / mass spectrometry

Abstract

Green juices from biorefinery original raw material (wild mix grass and alfalfa after wet fractionation and protein separation) have been investigated by gas chromatography/mass spectrometry. The carbohydrates, involved in the green juices, were derivatized and identified by both their retention times in the gas chromatogram and EI mass spectra compared to those of pure reference compounds. Additionally, chemical ionization mass spectra were recorded for better characterizing the carbohydrates present. The carbohydrates which could thus be identified, were quantified by response signals with respect to that of the internal standard β-phenyl-D-glucopyranoside.