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Amperometric methylene blue-mediated sensor highly sensitive to hydrogen peroxide based on a composite membrane of regenerated silk fibroin and poly-vinyl alcohol as immobilization matrix for horseradish peroxidase

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Abstract

Horseradish peroxidase (HRP) was effectively entrapped in a novel composite membrane of poly-vinyl alcohol and regenerated silk fibroin, and IR was employed to provide a useful insight into the structure of the composite membrane. A methylene bluemediated sensor highly sensitive to hydrogen peroxide was constructed, which was based on the immobilization of HRP in the composite membrane. Cyclic voltammetry and amperometric measurement were utilized to demonstrate the feasibility of electron communication between immobilized HRP and a glassy carbon electrode in the bioelectrocatalytic reduction of hydrogen peroxide via methylene blue. Performance and characteristics of the sensor were evaluated with regard to response time, detection limit, selectivity, and dependence on temperature and pH as well as operating and storage stability. The sensor possesses a variety of characteristics including high sensitivity, rapid response time and a low detection limit of 0.1 μmol/L.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Shanghai University, 200 072, Shanghai, People’s Republic of China

    Haiying Liu & Deyao Qi

  2. Department of Chemistry, Fudan University, 200 433, Shanghai, People’s Republic of China

    Jianghong Qian & Jiagi Deng

  3. Department of Macromolecular Science, Fudan University, 200 433, Shanghai, People’s Republic of China

    Yongcheng Liu & Tongyin Yu

Authors
  1. Haiying Liu
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  2. Jianghong Qian
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  3. Yongcheng Liu
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  4. Tongyin Yu
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  5. Jiagi Deng
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  6. Deyao Qi
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Liu, H., Qian, J., Liu, Y. et al. Amperometric methylene blue-mediated sensor highly sensitive to hydrogen peroxide based on a composite membrane of regenerated silk fibroin and poly-vinyl alcohol as immobilization matrix for horseradish peroxidase. Z. Anal. Chem. 357, 302–307 (1997). https://doi.org/10.1007/s002160050157

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  • DOI: https://doi.org/10.1007/s002160050157

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