Skip to main content
SpringerLink
Log in

Entrapment of both glucose oxidase and peroxidase in regenerated silk fibroin membrane

Characterization of the membrane structure and its application to an amperometric glucose sensor employing methylene green as an electron transfer mediator

  • Original Paper
  • Biological Matrices
  • Published:
Fresenius' Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

Two enzmyes, glucose oxidase and peroxidase, were for the first time simultaneously immobilized in regenerated silk fibroin membrane. The structure and morphology of the regenerated silk fibroin membrane containing both glucose oxidase and peroxidase were investigated with IR spectra and SEM. The bienzymes do not change the structures of the regenerated silk fibroin in the membrane, which has an islands-sea structure. For the first time, an amperometric methylene green mediating sensor for glucose based on co-immobilization of both glucose oxidase and peroxidase in regenerated silk fibroin was constructed. Cyclic voltammetry and amperometry were used to test the suitability of methylene green shuttling electrons between peroxidase and the glassy carbon electrode. The bienzyme-based system offers fast response and high sensitivity of the sensor to glucose. The effects of pH, temperature, and the concentration of the mediator on the response current were evaluated, and the dependence of the Michaelis-Menten constant Km aPP on the concentration of the mediator was investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Updike SJ, Hicks GP (1976) Nature (London) 214: 986

    Article  Google Scholar 

  2. Turner APF (ed) (1991) Advances in biosensors vol, 1. JAI Press, London, Greenwich

    Google Scholar 

  3. Scheller FW, Schubert F (eds) (1992) Biosensors. Elsevier, Amsterdam

    Google Scholar 

  4. Clark LC, Jr (1979) Methods Enzymol 56: 448

    Article  CAS  Google Scholar 

  5. Ianniello RM, Yacynych AM (1981) Anal Chem 53: 2090

    Article  CAS  Google Scholar 

  6. Wang J, Naser N, Angnes L, Hui W, Chem L (1992) Anal Chem 64: 1285

    Article  CAS  Google Scholar 

  7. Chi Q, Dong S (1993) Anal Chim Acta 278: 17

    Article  CAS  Google Scholar 

  8. Bennetto HP, Dekeyzer DR, Delaney GM, Koshy A, Mason JR, Razack LA, Stirling JL, Thurston CF (1987) Analyst 8: 22

    Google Scholar 

  9. Kazuhara A, Asakura T, Tomoda R, Matsunaga T (1987) J Biotechnol 5: 199

    Article  Google Scholar 

  10. Asakura T, Yoshimizu H, Kuzuhara A, Matsunaga T (1988) J Seric Sci Jpn 57: 203

    CAS  Google Scholar 

  11. Demura M, Asakura T (1989) Biotechnol Bioeng 33: 598

    Article  CAS  Google Scholar 

  12. Demura M, Asakura T, Kurso T (1989) Biosensors 4: 361

    Article  CAS  Google Scholar 

  13. Demura M, Asakura T, Nakamura E, Tamura H (1989) J Biotechnol 10: 113

    Article  CAS  Google Scholar 

  14. Qian J, Liu Y, Liu H, Yu T, Deng J (1996) Fresenius J Anal Chem (in press)

  15. Liu Y, Liu H, Qian J, Deng JQ, Yu T (1996) Anal Chim Acta (in press)

  16. Qian J, Liu Y, Liu H, Yu T, Deng J (1996) J Electroanal Chem (in press)

  17. Liu H, Qian J, Liu Y, Yu T, Deng J (1996) Anal Proc (submitted)

  18. Kamin RA, Wilson GS (1980) Anal Chem 52: 1198

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Macromolecular Science, Fudan University, 200433, Shanghai, People’s Republic of China

    Yongcheng Liu & Tongyin Yu

  2. Department of Chemistry, Fudan University, 200433, Shanghai, People’s Republic of China

    Haiying Liu, Jianghong Qian & Jiaqi Deng

Authors
  1. Yongcheng Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haiying Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianghong Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiaqi Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tongyin Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, Y., Liu, H., Qian, J. et al. Entrapment of both glucose oxidase and peroxidase in regenerated silk fibroin membrane. Fresenius J Anal Chem 355, 78–82 (1996). https://doi.org/10.1007/s0021663550078

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s0021663550078

Keywords

Search

Navigation