Summary
A HPLC-method for the determination of pyridoxyl alkaloids — endogenous condensation products of vitamin B6 and biogenic amines, resp. amino acids — is presented. On the basis of ion-pair assisted reversed-phase HPLC, conditions were found for the first time that allow an efficient separation and detection of these — partially extremely polar — heterocycles even from complex biofluids like mammalian blood and urine. The method allows to investigate the condensation kinetics in vitro, and — in combination with radiochemical methods — to prove the formation of pyridoxyl alkaloids in vivo. In single cases, the analysis is hampered by other constituents of blood or urine; the detection limit (S/N = 2) for a 250 μl test volume under optimum conditions is 1–2 ng.
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Literatur
Schöpf C, Bayerle H (1934) Liebigs Ann Chem 513:190
Piclet A, Spengler T (1911) Chem Ber 44:2030
Whaley W, Govindachari T (1951) Org React 6:151
Holtz P, Stock K, Westermann F (1964) Nature (Lond) 203:656
Collins MA (1977) In: Blum K (ed) Alcohol and opiates. Academic Press, New York, p 155
Bringmann G (1979) Naturwissenschaften 66:22
Collins MA (1983) In: Brossi A (ed) The alkaloids 21:329
Bringmann G, Schneider S, Hille A (1986) Nachr Chem Tech Lab 34:222
Borri Votattorni C, Orlacchio A, Giartosio A, Conti F, Turano C (1975) Eur J Biochem 53:151
Ebadi M, Govitrapong P (1979) J Neurochem 32:845
Rudd EA, Cunningham WC, Thanassi JW (1979) J Med Chem 22:233
Fellman JH, Roth ES (1971) Biochemistry 10:408
Maslinski C, Kierska D, Kling K (1971) Histamine Proc Satell Symp, p 270
Kita E, Kierska D, Maslinski C (1977) Rocz Chem 51:1741
Kierska D, Sasiak K, Maslinski C (1978) Agents Actions 8:474
Kita E, Maslinski C (1979) Pol J Chem 53:1211
Casella L, Gulotti M (1981) J Am Chem Soc 103:6338
Koszuk E, Kierska D, Maslinski C (1978) Agents Actions 8:185
Bringmann G, Schneider S (1986) Angew Chem 98:167; (1986) Angew Chem Int Engl Ed 25:177
Kierska D, Sasiak K, Maslinski C (1981) Agents Actions 11:28
Sandler M, Carter SB, Hunter K, Stern G (1973) Nature (Lond) 241:439
Turner AJ, Baker KM, Algeri S, Frigerio A, Garattini S (1974) Life Sci 14:2247
Robbins J (1968) Clin Res 16:554
Kenyhercz TM, Kissinger PT (1978) J Pharm Sci 67:112
Sebrell WH, Harris RS (1968) The vitamins, 2. Aufl. Academic Press, New York
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Diese Arbeit ist Herrn Professor Dr. Helmut Dörfel, BASF AG, Ludwigshafen, zum 60. Geburstag gewidmet.
Teil 5: Bringmann G, Hille A, Zsiška M (1987) Heterocycles, im Druck
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Bringmann, G., Schneider, S. & Richter, K.D. HPLC-Analytik hochpolarer Pyridoxyl-Alkaloide im Säugerblut. Z. Anal. Chem. 329, 480–484 (1987). https://doi.org/10.1007/BF00480092
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DOI: https://doi.org/10.1007/BF00480092