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Differential pulse polarographic determination of citric acid with a soluble enzyme preparation

Differentialpuls-polarographische Bestimmung von Citronensäure mit Hilfe eines löslichen Enzympräparats

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  • Food, Pharmaceutical and Biological Material
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Summary

An electrochemical method for the specific determination of citric acid in sports drink and plaster using citrate lyase and oxaloacetate decarboxylase has been studied. The technique is reasonably rapid and simple to perform. Results from recovery experiments are excellent, i.e. recovery is 99.7±3.0% at a confidence limit of 95% (n=3), and reproducibility (R.S.D.) at 20 μmol/l citric acid is 1.46% (n=3). The method can possibly be used for monitoring citric acid in clinical and food-processing samples.

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Author notes

  1. Teiji Kakizaki

    Present address: Chemical Laboratory, Iwamizawa Branch, Hokkaido University of Education, Iwamizawa 068, Japan

Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060, Japan

    Kiyoshi Hasebe, Satoshi Hikima, Teiji Kakizaki & Hitoshi Yoshida

Authors
  1. Kiyoshi Hasebe
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  2. Satoshi Hikima
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  3. Teiji Kakizaki
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  4. Hitoshi Yoshida
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Hasebe, K., Hikima, S., Kakizaki, T. et al. Differential pulse polarographic determination of citric acid with a soluble enzyme preparation. Z. Anal. Chem. 333, 19–22 (1989). https://doi.org/10.1007/BF00572610

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  • DOI: https://doi.org/10.1007/BF00572610

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