Abstract
Prosthecae were sheared from cells ofAsticcacaulis biprosthecum with a Sorvall omnimixer and purified by centrifugation. Removal of prosthecae resulted in no loss in viability. Purified prosthecae contain protein, carbohydrates and lipids. Certain enzymes that are present in the main body of the cell are also present in prosthecae: malic dehydrogenase (E.C. 1.1.1.37), alkaline phosphatase (E. C. 3.1.3.1), succinic dehydrogenase (E.C. 1.3.99.1), cytochromec reductase (E.C. 1.6.2.a), and cytochromes. Other enzymes present in whole cells are not in prosthecae: isocitric dehydrogenase (E.C. 1.1.1.41), NADH oxidase (E.C. 1.6.99.3), glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49), and lactic dehydrogenase (E.C. 1.1.2.3). Swarmer cells (non-prosthecate) were purified by allowing prosthecate cells to attach to cheesecloth in the growth medium. Envelopes prepared from prosthecae were compared with envelope from swarmer cells. The two envelope fractions differed in the disc-gel electrophoretic patterns of proteins solubilized from them.
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References
Bergmeyer, H. U., Bernt, E.: Malic dehydrogenase. In: Methods of enzymatic analysis, H. U. Bergmeyer, Ed., pp. 757–760. New York-London: Academic Press 1965
Bergmeyer, H. U., Bernt, E., Hess, B.: Lactic dehydrogenase. In: Methods of enzymatic analysis, H. U. Bergmeyer, Ed., pp. 736–743. New York-London: Academic Press 1965
Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1956)
Cohen-Bazire, G., Kunisawa, R., Poindexter, J. S.: The internal membranes ofCaulobacter crescentus. J. gen. Microbiol.42, 301–308 (1966)
Czok, R.: Magnesium in plasma. In: Methods of enzymatic analysis, H. U. Bergmeyer, Ed., pp. 640–643. New York-London: Academic Press 1965
Degnen, S. T., Newton, A.: Chromosome replication during development inCaulobacter crescentus. J. molec. Biol.64, 671–680 (1972)
Demoss, R. D.: Glucose-6-phosphate and 6-phosphogluconate dehydrogenase fromLeuconostoc mesenteroides. In: Methods in enzymology, S. P. Colowick and N. O. Kaplan, Eds., Vol. 1, pp. 328–334. New York-London: Academic Press 1955
Dulaney, J. T., Touster, O.: The solubilization and gel electrophoresis of membrane enzymes by use of detergents. Biochim. biophys. Acta (Amst.)196, 29–34 (1970)
Fortnagel, P., Freeze, E.: Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. J. Bact.95, 1431–1438 (1968)
Kaback, H. R.: Transport across isolated bacterial cytoplasmic membranes. Biochim. biophys. Acta (Amst.)265, 367–416 (1972)
Kerr, S. E., Seraidarium, K.: The separation of purine nucleosides from free purines and the determination of the purines and release in these fractions. J. biol. Chem.159, 211–225 (1945)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
Malamy, M., Horecker, B. L.: The localization of alkaline phosphatase inEscherichia coli. Biochem. biophys. Res. Commun.5, 104–108 (1961)
Pate, J. L., Ordal, E. J.: The fine structure of two unusual stalked bacteria. J. Cell Biol.27, 133–150 (1965)
Pate, J. L., Porter, J. S., Jordan, T. L.:Asticcacaulis biprosthecum: life cycle, morphology and cultural characteristics. Antonie v. Leeuwenhoek39, 569–583 (1973)
Poindexter, J. S.: Biological properties and classification of the caulobacter group. Bact. Rev.28, 231–295 (1964)
Reavely, D. A., Rogers, N. J.: Some enzymatic activities and chemical properties of mesosomes and cytoplasmic membranes ofBacillus licheniformis 6346. Biochem. J.113, 67–79 (1969)
Rieske, J. S.: The quantitative determination of mitochondrial hemo-proteins. In: Methods in enzymology, S. P. Colowick, N. O. Kaplan, Eds., Vol. 10, pp. 488–494. New York-London: Academic Press 1967
Roe, J. H., Dailey, R. E.: Determination of glycogen with the anthrone reagent. Analyt. Biochem.15, 245–250 (1966)
Schmidt, J. M., Stanier, R. Y.: The development of cellular stalks in bacteria. J. biol. Chem.28, 423–436 (1966)
Weibull, C., Beckman, H., Bergstrom, L.: Localization of enzymes inBacillus megaterium, strain M. J. gen. Microbiol.20, 519–531 (1959)
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Jordan, T.L., Porter, J.S. & Pate, J.L. Isolation and characterization of prosthecae ofAsticcacaulis biprosthecum . Arch. Microbiol. 96, 1–16 (1974). https://doi.org/10.1007/BF00590158
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DOI: https://doi.org/10.1007/BF00590158