Skip to main content
Log in

Mikrobielle Verwertung von Methanol

Eigenschaften der Formaldehyddehydrogenase und der Formiatdehydrogenase aus Candida boidinii

Microbial assimilation of methanol

Properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii

  • Kurze Mitteilungen
  • Published:
Archiv für Mikrobiologie Aims and scope Submit manuscript

Summary

The oxidation of formaldehyde to carbon dioxide in cell-free extracts of methanol-grown Candida boidinii has been investigated. A specific NAD-dependent formaldehyde dehydrogenase requiring reduced glutathione has been partially purified. Furthermore, a NAD-linked formate dehydrogenase was found in cell-free extracts. The synthesis of these two enzymes is induced by methanol and repressed by glucose. The possible significance of these enzymes in the energy-generating system is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Literatur

  • Bray, G. A.: A single efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem. 1, 279–285 (1960).

    Google Scholar 

  • Campbell, L. L.: The oxidative degradation of glycine by a Pseudomonas. J. biol. Chem. 217, 669–673 (1955).

    Google Scholar 

  • Harrington, A. A., Kallio, R. E.: Oxidation of methanol and formaldehyde by Pseudomonas methanica. Canad. J. Microbiol. 6, 1–7 (1960).

    Google Scholar 

  • Johnson, P. A., Quayle, J. R.: Microbial growth on C1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM 1. Biochem. J. 93, 281–290 (1964).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).

    Google Scholar 

  • Quayle, J. R.: Formate dehydrogenase. In: S. P. Colowick and N. O. Kaplan, Eds., Methods in enzymology, Vol. IX, pp. 360–364. New York: Academic Press 1966.

    Google Scholar 

  • Quayle, J. R.: The metabolism of one-carbon compounds by microorganisms. Advanc. Microbiol. Physiol. 7, 119–203 (1972).

    Google Scholar 

  • Rose, Z. B., Racker, E.: Formaldehyde dehydrogenase from baker's yeast. J. biol. Chem. 237, 3279–3281 (1962).

    Google Scholar 

  • Sahm, H., Wagner, F.: Mikrobielle Verwertung von Methanol. Isolierung und Charakterisierung der Hefe Candida boidinii. Arch. Mikrobiol. 84, 29–42 (1972).

    Google Scholar 

  • Sahm, H., Wagner, F.: Microbial assimilation of methanol. The ethanol- and methanol-oxidizing enzymes of the yeast Candida boidinii. Europ. J. Biochem. (im Druck, 1973).

  • Strittmatter, P., Ball, E. G.: Formaldehyde dehydrogenase, a glutathione dependentenzyme system. J. biol. Chem. 213, 445–461 (1955).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sahm, H., Wagner, F. Mikrobielle Verwertung von Methanol. Archiv. Mikrobiol. 90, 263–268 (1973). https://doi.org/10.1007/BF00424978

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00424978

Navigation