Abstract
The rate of synthesis and secretion of exo-1–3-β-glucanase activity closely paralleled the specific rate of growth in exponentially growing Saccharomyces cerevisiae cells in batch culture. When the stationary phase was reached both synthesis and secretion stopped. No activity was synthesized when the cells were maintained in carbon sources that did not allow them to grow. Studies in continuous culture indicate a strong relationship between the synthesis of exoglucanase activity and the specific growth rate. These results are taken as evidence of an essential role of this activity during the yeast budding cycle.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- p-NPG:
-
p-nitrophenyl-β-d-glucopyranoside
- Sr :
-
glucose concentration in the sterile reservoir; \(\bar s\), glucose concentration at the steady state
- \(\bar x\) :
-
biomass density at the steady state
- G :
-
glucanase activity
- Q g :
-
specific exoglucanase synthesis rate
References
Bartnicki-García S (1973) Fundamental aspects of hyphal morphogenesis. Symp Soc Gen Microbiol 23:245–267
Bartnicki-García S, McMurrough I (1971) Biochemistry of morphogenesis in yeasts. In: Rose AH, Harrison JH (eds) The yeasts, vol II. Academic Press, New York, pp 441–491
Farkas V, Biely P, Bauer S (1973) Extracellular β-glucanases of the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 321:246–255
Fleet GH, Phaff HJ (1981) Fungal glucan-structure and metabolism. In: Tanner W, Loewus FA (eds) Encyclopedia of plant physiology. New Series, vol 13B. Springer, Berlin Heidelberg New York, pp 416–440
Hernandez LM, Olivero I, Larriba G (1983) Detection of inactive precursors of β-glucanases in Saccharomyces cerevisiae. FEBS Lett 161:190–194
Matin A (1981) Regulation of enzyme synthesis as studied in continuous culture. In: Calcot PH (ed) Continuous culture of cells, vol 2. CRC Press Inc, Boca Raton, Florida, pp 69–97
Monod J (1950) La technique de culture continue; theorie et applications. Ann Inst Pasteur 19:390–410
Nombela C, Santamaria C (1984) Genetics of yeast cell wall autolysis. In: Nombela C (ed) Microbial cell wall synthesis and autolysis. Elsevier, Amsterdam New York Oxford, pp 239–248
Novick A, Szilard L (1950) Description of the chemostat. Science 112:715
Rey F del, Garcia-Acha I, Nombela C (1979) The regulation of β-glucanase synthesis in fungi and yeast. J Gen Microbiol 110:83–89
Sanchez A, Villanueva JR, Villa TG (1982) Saccharomyces cerevisiae secretes 2 exo-β-glucanases. FEBS Lett 138:209–212
Santos T, Rey F del, Conde J, Villanueva JR, Nombela C (1979) Saccharomyces cerevisiae mutant deficient in exo-1,3-β-glucanase production. J Bacteriol 139:333–338
Toda K (1976) Dual control of invertase synthesis in chemostat culture. Biotechnol Bioeng 18:1117–1124
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Olivero, I., Hernandez, L.M. & Larriba, G. Regulation of β-exoglucanase activity production by Saccharomyces cerevisiae in batch and continuous culture. Arch. Microbiol. 143, 143–146 (1985). https://doi.org/10.1007/BF00411037
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00411037