Abstract
A sterile continuous turbidostat culture in a 2-1 fermenter was used to systematically measure the gas exchange rates of Anacystis nidulans in a highly turbulent system under strictly controlled environmental conditions. An extensive physiological characterization of Anacystis is given in terms of photosynthesis rates (CO2 uptake and O2 evolution) and dark respiration rates as function of different parameters such as stirrer speed, temperature, CO2 and O2 concentration, light intensity, culture density and pH. Steady state ATP levels and apparent photophosphorylation rates complete the performance data. The dependence of the photosynthetic quotient from the parameters enables a physiological characterization of the light dependent nitrate assimilation.
Similar content being viewed by others
References
Batterton, J. C., VanBaalen, C.: Phosphorus deficiency and phosphate uptake in the blue-green alga Anacystis nidulans. Can. J. Microbiol. 14, 314–348 (1968)
Beevers, L., Hageman, R. H.: The role of light in nitrate metabolism in higher plants. In: Photophysiology, Vol. VII (A. C. Giese, ed.), pp. 85–113, New York: Academic Press 1972
Biggins, J.: Respiration in blue-green algae. J. Bact. 99, 570–575 (1969)
Bornefeld, T., Simonis, W.: Effects of light, temperature, pH and inhibitors on the ATP level of the blue-green alga Anacystis nidulans. Planta 115, 309–318 (1974)
Büchel, K. H., Röchling, H., Baedelt, H., Gerhardt, B., Trebst, A.: Hemmung der Photosynthese in Anacystis durch Alkylbenzimidazole. Z. Naturforsch. 22b, 535–537 (1967)
Csatorday, K., Horváth, G.: Synchronization of Anacystis nidulans. Oxygen evolution during the cell cycle. Arch. Microbiol. 111, 245–246 (1977)
Fogg, G. E., Stewart, W. D. P., Fay, P., Walsby, A. E.: The blue-green algae. London, New York: Academic Press 1973
Frischknecht, K., Schneider, K.: An improved bioluminescence ATP analysis for extraction-resistant algal cells. Arch. Hydrobiol. (in press, 1979)
Galkina, T. B., Kashkovskiy, O. A., Lebedeva, Ye, K., Meleshko, G. I., Ul'yanin, Yu. N.: Characteristics of growth and gas exchange of the alga Anacystis nidulans in intensive culture. In: NASA Techn. Transl. (529), pp. 453–458 (1969)
Goldman, J. C., Porcella, D. B., Middlebrooks, E. J., Toerien, D. F.: The effect of carbon on algal growth — its relationship to eutrophication. Wat. Res. 6, 637–679 (1972)
Herdmann, M., Faulkner, B. M., Carr, N. G.: Synchronous growth and genome replication in the blue-green alga Anacystis nidulans. Arch. Mikrobiol. 73, 238–249 (1970)
Ihlenfeldt, M. J. A., Gibson, J.: CO2 fixation and its regulation in Anacystis nidulans (Synechococcus). Arch. Microbiol. 102, 13–21 (1975)
Jablonka, H.-J.: Reaktionsfunktionen für die Dimensionierung von biologischen Photoreaktoren. Ph. D. Thesis, Technical University of Munich (1978)
Kratz, W. A., Myers, J.: Photosynthesis and respiration of three blue-green algae. Pl. Physiol. 30, 275–280 (1955a)
Kratz, W. A., Myers, J.: Nutrition and growth of several blue-green algae. Am. J. Bot. 42, 282–287 (1955b)
Märkl, H., Vortmeyer, D.: Berechnung der Verteilung von Bestrahlungsstärke und Absorption in einem diffus von außen beleuchteten zylindrischen Photoreaktor. Chemie-Ing.-Techn. 47, 31 (1975)
Märkl, H.: CO2 transport and photosynthetic productivity of a continuous culture of algae. Biotechnol. Bioeng. 19, 1851–1862 (1977)
Moss, B.: The influence of environmental factors on the distribution of freshwater algae: an experimental study. II. The role of pH and the carbon dioxide-bicarbonate system. J. Ecol. 61, 157–177 (1973)
Ogawa, T., Shibata, K.: A sensitive method for determining chlorophyll b in plant extracts. Photochem. Photobiol. 4, 193–200 (1965)
Raven, J. A.: Exogenous inorganic carbon sources in plant photosynthesis. Biol. Rev. 45, 167–221 (1970)
Schneider, K., Frischknecht, K.: Determination of the O2 and CO2 KL a values in fermenters with the dynamic method measuring the step responses in the gas phase. J. appl. Chem. Biotechnol. 27, 631–642 (1977)
Slater, J. H.: The control of carbon dioxide assimilation and ribulose 1,5-diphosphate carboxylase activity in Anacystis nidulans grown in a light-limited chemostat. Arch. Microbiol. 103, 45–49 (1975)
VanBaalen, C.: Quantitative surface plating of coccoid blue-green algae, J. Phycol. 1, 19–22 (1965)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Frischknecht, K., Schneider, K. Physiological performances of the blue-green alga Anacystis nidulans in continuous turbidostat fermenter culture. Arch. Microbiol. 120, 215–221 (1979). https://doi.org/10.1007/BF00423068
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00423068