Abstract
A gene controlling fruit sucrose accumulation, sucr, was introgressed from the wild tomato species Lycopersicon chmielewskii into the genetic background of a hexose-accumulating cultivated tomato, L. esculentum. During introgression, the size of the L. chmielewskii chromosomal segment containing sucr was reduced by selection for recombination between RFLP markers for the sucr gene and flanking loci. The effects of sucr on soluble solids content, fruit size, yield and other fruit parameters were studied in the genetic background of the processing tomato cultivar ‘Huntl00’. In a segregating BC5F2 generation, the smallest introgression containing sucr-associated markers was necessary and sufficient to confer high-level sucrose accumulation, the effects of which were completely recessive. Fruit of sucr/sucr genotypes were smaller than those of +/sucr or +/+ genotypes at all stages of development. The timing of sugar accumulation and total sugar concentration were unaffected by sugar composition. No differences in total fruit biomass (fresh weight of red and green fruit) at harvest were observed between the genotypes, and sucrose accumulators produced greater numbers of fruit than hexose accumulators in one family. However, the proportion of ripe fruit at harvest, and hence yield of ripe fruit, as well as average ripe fruit weight and seed set were reduced in sucr/sucr genotypes. Sucrose accumulation was also associated with increased soluble solids content, consistency, serum viscosity, predicted paste yield and acidity, and decreased color rating. In the first backcross to L. chmielewskii, hexose accumulators (+/sucr) had larger fruit than sucrose accumulators (sucr/sucr), while no difference in soluble solids was detected.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Azanza F, Young TE, Kim D, Tanksley SD, Juvik JA (1994) Characterization of the effect of introgressed segments of chromosome 7 and 10 from Lycopersicon chmielewskii on tomato soluble solids, pH, and yield. Theor Appl Genet 87:965–972
Bruyn JW De, Garretsen F, Kooistra E (1971) Variation in taste and chemical composition of the tomato (Lycopersicon esculentum Mill.). Euphytica 20:214–227
Bryce WH, Nelson OE (1979) Starch-synthesizing enzymes in the endosperm and pollen of maize. Plant Physiol 63:312–317
Chetelat RT, Klann E, DeVerna JW, Yelle S, Bennett AB (1993) Inheritance and genetic mapping of fruit sucrose accumulation in Lycopersicon chmielewskii. Plant J 4:643–650
Chetelat RT, DeVerna JW, Bennett AB (1995) Introgression into tomato (Lycopersicon esculentum) of the L. chmielewskii sucrose accumulator gene (sucr) controlling fruit sugar composition. Theor Appl Genet 91:327–333
Dali N, Michaud D, Yelle S (1992) Evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. Plant Physiol 99: 434–438
Damon S, Hewitt J, Neider M, Bennett AB (1988) Sink metabolism in tomato fruit. II. Phloem unloading and sugar uptake. Plant Physiol 87:731–736
Davies JN, Hobson G (1981) The constituents of tomato fruit — the influence of environment, nutrition and genotype. CRC Crit Rev Food Sci Nutr 15:205–280
Davies JN, Kempton RJ (1975) Changes in the individual sugars of tomato fruit during ripening. J Sci Food Agric 26:1103–1110
Dempsey WH, Boynton JE (1965) Effect of seed number on tomato fruit size and maturity. J Am Soc Hortic Sci 86:575–581
Dinar M, Stevens MA (1981) The relationship between starch accumulation and soluble solids content of tomato fruits. J Am Soc Hortic Sci 106:415–418
Elliott KJ, Butler WO, Dickinson CD, Konno Y, Vedvick TS, Fitzmaurice L, Mirkov TE (1993) Isolation and characterization of fruit vacuolar invertase genes from two tomato species and temporal differences in mRNA levels during fruit ripening. Plant Mol Biol 21:515–524
Estruch JJ, Beltran JP (1991) Changes in invertase activities precede ovary growth induced by gibberellic acid in Pisum sativum. Physiol Plant 81:319–326
Klann EM, Chetelat RT, Bennett AB (1993) Expression of acid invertase gene controls sugar composition in tomato (Lycopersicon) fruit. Plant Physiol 103:863–870
Koskitalo LN, Ormrod DP (1972) Effects of sub-optimal ripening temperatures on the color quality and pigment composition of tomato fruit. J Food Sci 37:56–59
Muller-Rober B, Sonnewald W, Willmitzer L (1992) Inhibition of the ADP-glucose pyrophosphorylase in transgenic potatoes leads to sugar-storing tubers and influences tuber formation and expression of tuber storage protein genes. EMBO J 11:1229–1238
Osborn TC, Alexander DC, Fobes JF (1987) Identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. Theor Appl Genet 73: 350–356
Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete RFLP linkage map. Nature 335: 721–726
Paterson AH, DeVerna JW, Lanini B, Tanksley SD (1990) Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics 124:735–742
Poysa V (1993) Use of Lycopersicon cheesmanii and L. chmielewskii to increase dry matter content of tomato fruit. Can J Plant Sci 73:273–279
Reger BJ, Pressey R, Chaubal R (1992) In vitro chemotropism of pearl millet pollen tubes to stigma tissue: a response to glucose produced in the medium by tissue-bound invertase. Sex Plant Reprod 5:201–205
Rick CM (1974) High soluble-solids content in large-fruited tomato lines derived from a wild green-fruited species. Hilgardia 42: 493–510
Stebbins GL (1958) The inviability, weakness, and sterility of interspecific hybrids. Adv Genet 9:147–215
Stevens MA (1986) Inheritance of tomato fruit quality components. Plant Breed Rev 4:273–311
Stevens MA, Kader AA, Albright-Holton M, Algazi M (1977) Genotypic variation for flavor and composition in fresh market tomatoes. J Am Soc Hortic Sci 102:680–689
Tanksley SD, Hewitt J (1988) Use of molecular markers in breeding for soluble solids content in tomato — a re-examination. Theor Appl Genet 75:811–823
Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder M, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160
Tieman DM, Harriman RW, Ramamohan G, Handa AK (1992) An antisense pectin methylesterase gene alters pectin chemistry and soluble solids in tomato fruit. Plant Cell 4:667–679
Walker AJ, Ho LC, Baker DA (1978) Carbon translocation in the tomato: pathways of carbon metabolism in the fruit. Ann Bot 42:901–909
Yelle S, Hewitt JD, Robinson NL, Damon S, Bennett AB (1988) Sink metabolism in tomato fruit. III. Analysis of carbohydrate assimilation in a wild species. Plant Physiol 87:737–740
Yelle S, Chetelat RT, Dorais M, DeVerna JW, Bennett AB (1991) Sink metabolism in tomato fruit. IV. Genetic and biochemical analysis of sucrose accumulation. Plant Physiol 95:1026–1035
Author information
Authors and Affiliations
Additional information
Communicated by M. Koornneef
Rights and permissions
About this article
Cite this article
Chetelat, R.T., DeVerna, J.W. & Bennett, A.B. Effects of the Lycopersicon chmielewskii sucrose accumulator gene (sucr) on fruit yield and quality parameters following introgression into tomato. Theoret. Appl. Genetics 91, 334–339 (1995). https://doi.org/10.1007/BF00220896
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220896