Skip to main content
SpringerLink
Log in

Somatic embryogenesis in white spruce (Picea glauca): genetic control in somatic embryos exposed to storage, maturation treatments, germination, and cryopreservation

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Genetic controls for growth of embryogenic cultures, storage, maturation treatments, germination and cryopreservation in white spruce somatic embryogenesis (SE) were examined. These SE processes were under genetic control but less strongly so than the initiation phase. For all the SE characters examined, variance due to clones within families was significant and often the largest genetic component of variance. This was further partitioned using an additive-dominance-epistasis model. A relatively-large proportion of the total genetic variance was due to epistatic variance in the maturation and germination of somatic embryos. Embryogenic lines were cryopreserved easily without a distinct genetic influence being noticed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson RL, Bancroft TA (1952) Statistical theory in research. McGraw-Hill, New York

    Google Scholar 

  • Attree SM, Tautorus TE, Dunstan DI, Fowke LC (1990) Somatic embryo maturation, germination, and soil establishment of plants of black and white spruce (Picea mariana and Picea glauca). Can J Bot 68:2583–2589

    Article  MATH  Google Scholar 

  • Bonga JM, von Aderkas P (1993) Rejuvenation of tissue from mature conifer and its implications for propagation in vitro. In: Ahuja MR, Libby WJ (eds) Clonal forestry I: genetics and biotechnology. Springer-Verlag, Berlin, pp 182–199

    Google Scholar 

  • Bryce GR (1980) Data analysis in RUMMAGE — a user's guide. Department of Statistics, Brigham Young University, Provo, Utah

    Google Scholar 

  • Charest PJ, Klimaszewwska K, Ward C (1993) Cryopreservation of germplasm of Larix and Picea species. In: Bajaj YSP (ed) Biotechnology in agriculture and forestry, vol 26. Springer-Verlag, Berlin

    Google Scholar 

  • Dunstan DI, Bekkaoui F, Pilon M, Fowke LC, Abrams SR (1988) Effects of ABA and analogues on the maturation of white spruce (Picea glauca) somatic embryos. Plant Sci 58:77–84

    Google Scholar 

  • Hakman I, von Arnold S (1988) Somatic embryogenesis and plant regeneration from suspension cultures of Picea glauca (white spruce). Physiol Plant 72:579–587

    Google Scholar 

  • Lerner IM (1958) The genetic basis of selection. John Wiley and Sons, New York

    Google Scholar 

  • Libby WJ, Ahuja MR (1993) Clonal forestry. In: Ahuja MR, Libby WJ (eds) Clonal forestry I: Genetics and biotechnology. Springer-Verlag, Berlin, pp 1–8

    Google Scholar 

  • Mayer U, Ruiz RAT, Berleth T, Miséra, S, Jürgens G (1991) Mutations affecting body organization in the Arabidopsis embryo. Nature 353:402–407

    Google Scholar 

  • Mullin TJ, Park YS (1992) Estimating genetic gains from alternative breeding strategies for clonal forestry. Can J For Res 21:14–23

    Google Scholar 

  • Mullin TJ, Morgenstern EK, Park YS, Fowler DP (1992) Genetic parameters from a clonally-replicated test of black spruce (Picea mariana). Can J For Res 22:24–36

    Google Scholar 

  • Park YS, Pond SE, Bonga JM (1993) Initiation of somatic embryogenesis in white spruce (Picea glauca): genetic control, culture treatment effects, and implication for breeding. Theor Appl Genet 86:427–436

    Google Scholar 

  • Roberts, DR, Flinn BS, Webb DT, Webster FB, Sutton BCS (1990 a) Abscisic acid and indole-3-butyric acid regulation of maturation and accumulation of storage proteins in somatic embryos of interior spruce. Physiol Plant 78:355–360

    Google Scholar 

  • Roberts DR, Sutton BCS, Flinn BS (1990 b) Synchronous and high frequency germination of interior spruce somatic embryos following partial drying at high relative humidity. Can J Bot 68:1086–1090

    Google Scholar 

  • Satterthwaite FE (1946) An approximate distribution of estimates of variance components. Biom Bull 2:110–114

    Google Scholar 

  • Schaffer HE, Usanis RA (1969) General least squares analysis of diallel experiments: a computer program — DIALL. NC State Univ Genet Dep Res Rep No. 1, Raleigh, North Carolina

  • Sokal RR, Rohlf FJ (1981) Biometry. Freeman and Co, New York

    Google Scholar 

  • Tietjen GL (1974) Exact and approximate tests for unbalanced random effects designs. Biometrics 30:573–581

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Canadian Forest Service — Maritimes Region, Natural Resources Canada, P.O. Box 4000, E3B 5P7, Fredericton, New Brunswick, Canada

    Y. S. Park, S. E. Pond & J. M. Bonga

Authors
  1. Y. S. Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. E. Pond
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. M. Bonga
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by P. M. A. Tigerstedt

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, Y.S., Pond, S.E. & Bonga, J.M. Somatic embryogenesis in white spruce (Picea glauca): genetic control in somatic embryos exposed to storage, maturation treatments, germination, and cryopreservation. Theoret. Appl. Genetics 89, 742–750 (1994). https://doi.org/10.1007/BF00223714

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00223714

Key words

Search

Navigation