Skip to main content
SpringerLink
Log in

Differential effects of carbohydrate intake on cardiac myosin isoform expression in normal weanling and adult rats

  • Original Article
  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Dietary manipulations involving high carbohydrate feeding increase VI cardiac myosin isoform expression in hormonally deficient rats. The purpose of this study was to determine if extremes in dietary carbohydrate availability could alter cardiac myosin isoform patterns in normal weanling and adult rats. Three and six weeks of dietary manipulations (either high or low carbohydrate diets) failed to change calcium-activated myofibril ATPase activity, calcium regulated myofibril ATPase activity, or the myosin isoform distribution in the adult. In contrast, a four week, high carbohydrate diet reduced calcium activated myosin ATPase activity by 33%, calcium regulated myofibril ATPase activity by 10%, and Vl isoform expression by 66% in weanling rats. Although the low carbohydrate diet caused no change in the myosin ATPase properties, it decreased VI isoform expression by 17%. These results show that carbohydrate availability can alter cardiac myosin isoform expression in normal rats, but only at weanling age. The reason for this age-related contrast in response to dietary manipulations is unknown at this stage. The dietary manipulations may have acted directly on the heart by creating a state of malnutrition, or indirectly, by altering some developmental process which links maturation of the sympathetic nervous system with myosin isoform expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Hoh JFY, McGrath PA, Hale PT Electrophoretic analysis of multiple forms of cardiac myosin: effect of hypophysectomy and thyroxine replacement. J Mol Cell Cardiol 10:1053–1076, 1978.

    Google Scholar 

  2. Hoh JFY, Yeoh GPS, Thomas MAW, Higginbottom L: Structural differences in the heavy chains of rat ventricular myosin isoenzymes. FEBS Letters 97:330–334, 1979

    Google Scholar 

  3. Mahadavi V, Periasamy M, Nadal-Ginard B: Molecular characterization of two myosin heavy chain genes expressed in adult heart. Nature 291:659–664, 1982

    Google Scholar 

  4. Lompre AM, Mercadier JJ, Wisenewsky C, Bouveret P, Pantaloni C, D'Albis A, Schwartz K: Species- and age- dependent changes in the relative amounts of cardiac myosin isoenzymes in mammals. Dev Biol 84:286–290, 1981

    Google Scholar 

  5. Capasso JM, Malhotra A, Scheuer J, Sonnenblick EH: Myocardial biochemical, contractile, and electrical performance and imposition of hypertension in young and old rats. Circ Res 58:445–460, 1986

    Google Scholar 

  6. Chizzonite RA, Zak R: Regulation of myosin isoenzyme composition in fetal and neonatal rat ventricle by endogenous thyroid hormones. J Biol Chem 259:12628–12632, 1984

    Google Scholar 

  7. Isumo S, Nadal-Ginard B, Mahadavi V: All members of the MHC multigene family respond to thyroid hormone id a highly tissue-specific manner. Science 231:597–600, 1986

    Google Scholar 

  8. Fein FS, Malhotra A, Miller-Green B, Scheuer J, Sonnenblick EH: Diabetic cardiomyopathy in rats: mechanical and biochemical response to different insulin doses. Am J Physiol 247:H817-H823, 1984

    Google Scholar 

  9. Dillmann WH: Influence of thyroid hormone administration on myosin ATPase activity and myosin isoenzyme distribution in the heart of diabetic rats. Metabolism 31:199–204, 1982

    Google Scholar 

  10. Garber DW, Everett AW, Neely JR: Cardiac function and myosin ATPase in diabetic rats treated with insulin, T3 and T4. Am J Physiol 244:H592-H598, 1983

    Google Scholar 

  11. Dillmann WH: Myosin isoenzyme distribution and calcium activated myosin ATPase activity in the rat heart is influenced by fructose feeding and triiodothyronine. Endocrinology 116:2160–2166, 1985

    Google Scholar 

  12. Sheer D, Morkin E: Myosin isoenzyme expression in rat ventricles: effects of thyroid hormone analogs, catacholamine, glucocorticoids, and high carbohydrate diet. J Pharm Exptl Therapeutics 229:872–879, 1984

    Google Scholar 

  13. Dillmann WH: Methyl palmoxirate increases calcium myosin ATPase acivity and changes myosin isoenzymes distribution in the diabetic rat heart. Am J Physiol 248:E602-E606, 1985

    Google Scholar 

  14. American Institute of Nutrition: Report of the AIN Ad Hoc Committee on standards for nutritional studies. J Nutr 107:1340–1348, 1977

    PubMed  Google Scholar 

  15. Solaro RJ, Pang DC, Briggs FN: The purification of cardiac myofibrils with Triton X-100. Biochim Biophys Acta 245:259–262, 1971

    Google Scholar 

  16. Gornall AG, Bardawill CJ, David MM: Determination of the serum proteins by the Biuret method. J Biol Chem 177:751–756, 1949

    Google Scholar 

  17. Tibbits GF, Barnard RJ, Baldwin KM, Cugalj N, Roberts NK: Influence of exercise on excitation-contraction coupling in rat myocardium. Am J Physiol 240:H472-H480, 1981

    Google Scholar 

  18. Fisk CH, Subarrow YJ: The colorometric determination of phorphorus. J Biol Chem 66:374–400, 1925

    Google Scholar 

  19. Srere PA: Citrate synthase.Methods Enzymol 13:3–5, 1969

    Google Scholar 

  20. Raabo E, Terkildsen TC: On the enzymatic determination of blood glucose. Scand J Clin Lab Invest 12:402, 1960

    Google Scholar 

  21. Baldwin KM, Campbell PJ, Hooker AM, Lewis RE: Effects of thyroid deficiency and sympathectomy on cardiac enzymes. Am J Physiol 236:C30-C34, 1979

    Google Scholar 

  22. Baldwin KM, Campbell PJ, Hooker AM, Lewis RE: Enzyme changes in neonatal skeletal muscle: effect of thyroid deficiency. Am J Physiol 235:C97-C102, 1978

    Google Scholar 

  23. Dillmann WH: Diabetes mellitus induces changes in cardiac myosin of the rat. Diabetes 29:579–582, 1980

    Google Scholar 

  24. Walker P, Dubois JB, Dussault JH: Free thyroid hormone concentrations during postnatal development in the rat. Pediatr Res 14:247–249, 1980

    Google Scholar 

  25. Dowell RT, Martin AF: Perinatal nutritional modification of weanling rat heart contractile protein. Am J Physiol 247:H967-H972, 1984

    Google Scholar 

  26. Samuel JL, Rapparot L, Syrovy I, Wisnewsky C, Marotto F, Whalen RG, Shwartz K: Differential effect of thyroxine on atrial and ventricular isomyosins in rats. Am J Physiol 250:H333-H341, 1986

    Google Scholar 

  27. Martin AF, Robinson DC, Dowell RT: Isomyosin and thyroid hormone levels in pressure-overloaded weanling and adult rat hearts. Am J Physiol 248:H305-H310, 1985

    Google Scholar 

  28. Lompre AM, Schwartz K, D'Albis A, Lacombe G, Thiem NV, Swynghedauw B: Myosin isoenzyme redistribution in chronic heart overload. Nature 282:105–107, 1979

    Google Scholar 

  29. Dillmann WH, Berry S, Alexander NM: A physiological dose of triiodothyronine normalized cardiac myosin adenosine triphosphatase activity and changed myosin isoenzyme distribution in semistarved rats. Endocrinology 112:2081–2087, 1983

    Google Scholar 

  30. MacIntosh AM, Mullin WM, Fitzsimmons DP, Herrick RE, Baldwin KM: Cardiac biochemical adaptations to exercise in sympathectomized neonatal rats. J Appl Physiol 60:991–996, 1986

    Google Scholar 

  31. Burger AG, Burger M, Wimpheimer K, Danforth Jr E: Interrelationships between energy metabolism and thyroid hormone metabolism during starvation in the rat. Acta Endocrinol 93:322–331, 1980

    Google Scholar 

  32. Rothwell NJ, Saville ME, Stock MJ: Sympathetic and thyroid influences on metabolic rate in fed, fasted, and refed rats. Am J Physiol 243:R339-R346, 1982

    Google Scholar 

  33. Sreter FA, Faris R, Balogh I, Somogyi E, Sotonyi P: Changes in myosin isoenzyme distribution induced by low doses of isoproterenol. Arch Int Pharmacodyn 260:159–164, 1982

    Google Scholar 

  34. Pauletto P, Libera LD, Vescovo G, Scanapiecco G, Angelini A, Passina AC, Palu CD: Propranolol- induced changes in ventricular isomyosin composition in the rat. Am Heart J 109:1269–1273, 1985

    Google Scholar 

  35. Bareis DL, Morgan RE, Lau C, Slotkin TA: Maturation of sympathetic neurotransmission in the rat heart. IV. Effects of guanethidine-induced sympathectomy on neonatal development of synaptic vesicles, synaptic terminal function, and heart growth. Dev Neurosci 4:15–24, 1981

    Google Scholar 

  36. Deskin R, Mills E, Whitmore WL, Seidler FJ, Slotkin TA: Maturation of sympathetic neurotransmission in the rat heart. VI. The effect of neonatal central catecholaminergic lesions. J Pharm Exptl Therapeutics 215:342–347, 1980

    Google Scholar 

  37. Landsberg L, Young JB: Fasting, feeding and regulation of the sympathetic nervous system. N Engl J Med 298:1295–1301, 1978

    Google Scholar 

  38. Vander Tuig JG, Romsos DR: Effects of dietary carbohydrate, fat, and protein on norepinephrine turnover in rats. Metabolism 33:26–33, 1984

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of California, 92717, Irvine, CA, USA

    G. Stephen Morris, Fadia Haddad & Kenneth M. Baldwin

Authors
  1. G. Stephen Morris
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fadia Haddad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kenneth M. Baldwin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Morris, G.S., Haddad, F. & Baldwin, K.M. Differential effects of carbohydrate intake on cardiac myosin isoform expression in normal weanling and adult rats. Mol Cell Biochem 78, 121–129 (1987). https://doi.org/10.1007/BF00229686

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00229686

Key words

Search

Navigation