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Vitamin A inhibits lipoperoxidation ascorbate-Fe++ dependent of rat kidney microsomes and mitochondria

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Abstract

In the present study it was investigated if Vitamin A supplementation could protect rat kidney microsomes and mitochondria from in vitro lipoperoxidation. After incubation of rat kidney microsomes and mitochondria in an ascorbate-Fe++ system, at 37°C during 60 min, it was observed that the total cpm/mg protein originated from light emission (chemiluminescence) was lower in those organelles obtained from the control group when compared with the vitamin A supplemented group. The fatty acid composition of microsomes and mitochondria from control group was profoundly modified when subjected to nonenzymatic lipoperoxidation with a considerable decrease of arachidonic acid, C20:4 (n-6) and docosapentaenoic acid, C22:5 (n−3) in mitochondria and docosahexaenoic acid C22:6 (n-3) in microsomes.As a consequence the peroxidizability index, a parameter based on the maximal rate of oxidation of specific fatty acids was higher in the supplemented animals than in those used as control. These results indicate that Vitamin A may act as antioxidant protecting rat kidney microsomes and mitochondria from deleterious effect.

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References

  1. Frei B: Reactive oxygen species and antioxidant vitamins: Mechanisms of action. Am J Med 97: 3A-5S, 3A–12S, 1994

    Google Scholar 

  2. Di Mascio P, Murphy ME, Sies H: Antioxidant defence systems: The role of carotenoids, tocopherols and thiols. Am J Clin Nutr 53: 194S-200S, 1991

    Google Scholar 

  3. Palmer S: Antioxidant vitamins and cancer risk. Nutr 10: 433–434, 1994

    Google Scholar 

  4. Aruoma OI: Nutrition and health aspects of free radicals and antioxidants. Fd Chem Toxic 32: 671–683, 1994

    Google Scholar 

  5. Bonorden WR, Pariza MW: Antioxidant nutrients and protection from free radicals. In: F.N. Kotsonis (ed.) Nutr Toxicol, Raven Press, 1994 pp 19–45

  6. Palacios A, Piergiacomi VA, Catalá A: Vitamin A supplementation inhibits chemiluminescence and lipid peroxidation in isolated rat liver microsomes and mitochondria. Mol Cell Biochem (in press), 1995

  7. Galleano M, Puntarulo S: Effect of mild iron overload on liver and kidney lipid peroxidation. Brazilian J Med Biol Res 27: 2349–2358, 1994

    Google Scholar 

  8. Radi R, Bush KM, Freeman BA: The role of cytochrome C and mitochondrial catalase in hydroperoxide-induced heart mitochondrial lipid peroxidation. Arch Biochem Biophys 300: 409–415, 1993

    Google Scholar 

  9. Yen TC, King KL, Lee HC, Yeh SH, Wei YH: Age dependent increase of mitochondrial DNA deletions together with lipid peroxides and superoxide dismutase in human liver mitochondria. Free Radic Biol Med 16: 207–214, 1994

    Google Scholar 

  10. Bollag W, Majewsky S, Jablonska S: Cancer combination chemotherapy with retinols: experimental rationale. Leukaemia 8: 1453–1457, 1994

    Google Scholar 

  11. Wright JR, Colby HD, Miles PR: Cytosolic factors which affect microsomal lipid peroxidation in lung and liver. Arch Biochem Biophys 206: 296–304, 1981

    Google Scholar 

  12. Galleano M, Puntarulo S: Hepatic chemiluminescence and lipid peroxidation in mild iron overload Toxicology 76: 27–38, 1992

    Google Scholar 

  13. Puntarulo S, Cederbaum AI: Stimulation of microsomal chemiluminescence by ferritin. Biochim Biophys Acta 1157: 1–8, 1993

    Google Scholar 

  14. Witing LA, Hornitt MK: Effect of degree of fatty acid unsaturation in tocopherol deficiency-induced creatinuria. J Nutr 82: 19–25, 1964

    Google Scholar 

  15. Tangen O, Jonsson J, Orrenius S: Isolation of rat liver microsomes by gel filtration chromatography. Analyt Biochem 54: 597–603, 1973

    Google Scholar 

  16. Cervato G, Viani B, Gatti P, Cestaro B: Studies on lipid peroxidation in different model membranes: role of cysteamine. Chem Phys Lipids 62: 31–38, 1992

    Google Scholar 

  17. Ohyashiky T, Sakata N, Mohri T, Matsui K: Fluorescence characteristics of peroxidation products in porcine intestinal brush-border membranes. Arch Biochem Biophys 284: 375–380, 1991

    Google Scholar 

  18. Wright JR, Rumbaugh RC, Colby HD, Miles PR: The relation between chemiluminescence and lipid peroxidation in rat hepatic microsomes. Arch Biochem Biophys 192: 344–351, 1979

    Google Scholar 

  19. Folch J, Lees N, Sloane Stanley GA: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 495–509, 1957

    Google Scholar 

  20. Pirozhkov SV, Eskelson CD, Watson RR, Hunter CG, Piotowsky JJ, Bernhard V: Effect of chronic consumption of ethanol and vitamin E on fatty acid composition and lipid peroxidation in rat heart tissue. Alcohol 9: 329–334, 1992

    Google Scholar 

  21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 193: 265–275, 1951

    Google Scholar 

  22. Godman VS, Huang HS, Shiratori T: Tissue distribution and metabolism of newly absorbed vitamin A in the rat. J Lipid Res 6: 390–396, 1965

    Google Scholar 

  23. Zhang LX, Cooney RV, Bertram JS: Carotenoids enhance gap junctional communications and inhibits lipid peroxidation of C3H/1OT1/2 cells: Relationship to their cancer chemopreventive action. Carcinogenesis 12: 2109–2114, 1991

    Google Scholar 

  24. Garewal HS: Precancerous lesions: role of antioxidant nutrients in preventing malignant transformation of cells. Vit Nutr Information Service 2: 1–11, 1992

    Google Scholar 

  25. Mobarhan S, Simms P, Anderson B: Effects of beta-carotene repletion on beta-carotene absorption, lipid peroxidation and neutrophil superoxide formation in young men. Nutr Cancer 14: 195–206, 1990

    Google Scholar 

  26. Olivieri O, Stanziai AM, Girelli D, Trevisan MT, Guarini P, Terzi M, Caffi S, Fontana F, Casaril M, Ferrari S, Corrocher R: Selenium status, fatty acids, vitamins A and E, and aging: the nove study. Am J Clin Nutr 60: 510–517, 1994

    Google Scholar 

  27. Bertram JS, Pung A, Churley M, Kappock TJ, Wilkins LR, Cooney RV: Diverse carotenoids protect against chemically induced neoplastic transformation. Carcinogenesis 12: 671–678, 1991

    Google Scholar 

  28. Sato N, Fujii K, Yuge O, Morio M: Changes in lipid peroxidation levels and lipid composition in lungs, livers, kidneys and brains of mice treated with parakuat. J Appl Toxicol 12: 365–368, 1992

    Google Scholar 

  29. Stohs SJ, Bagchi D: Oxidative mechanism in the toxicity of metal ions. Free Radic Biol Med 18: 321–336, 1995

    Google Scholar 

  30. Niki E, Yamamoto Y, Komuro E, Sato K: Membrane damage due to lipid oxidation. Am J Clin Nutr 53: 201S-205S, 1991

    Google Scholar 

  31. Morre DM: Intracellular actions of vitamin A. Int Rev Cytol 135: 139, 1992

    Google Scholar 

  32. Wang XD: Review: Absorption and metabolism of beta carotene. J Am Coll Nutr 13: 314–325, 1994

    Google Scholar 

  33. Olson JA: Carotenoids and vitamin A: an overview. Biochem and Clin Appl, Edited by Ong and Packed 178–192, 1992

  34. Alam SQ, Alam BS: Lipid peroxide alpha tocopherol and retinoid levels in plasma and liver of rats fed diets containing beta carotene and 1–3-cis-retinoic acid. J Nutr 113: 2608–2614, 1983

    Google Scholar 

  35. Ciaccio M, Valenza M, Tesoriere L, Bongiorno A, Albiero R, Livrea MA: Vitamin A inhibits doxorubicin-induced membrane lipid peroxidation in rat tissues in vivo. Arch Biochem Biophys 302: 103–108, 1993

    Google Scholar 

  36. Catalá A, Arcemis C, Cerruti A: Interaction of rat liver microsomes containing saturated or unsaturated fatty acids with fatty acid binding protein: peroxidation effect. Mol Cell Biochem 13: 135–139, 1994

    Google Scholar 

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Authors and Affiliations

  1. Assistant of the Comisión de Investigaciones Científicas y Técnicas de la Prov. de Buenos Aires (CIC), Argentina

    V. A. Piergiacomi

  2. Cátedra de Bioquímica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata CC 296, 1900 La Plata, Argentina

    A. Palacios

  3. Member of Carrera del Investigador Científico, Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Argentina

    A. Catalá

Authors
  1. V. A. Piergiacomi
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  2. A. Palacios
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  3. A. Catalá
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Piergiacomi, V.A., Palacios, A. & Catalá, A. Vitamin A inhibits lipoperoxidation ascorbate-Fe++ dependent of rat kidney microsomes and mitochondria. Mol Cell Biochem 165, 121–125 (1996). https://doi.org/10.1007/BF00229473

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  • DOI: https://doi.org/10.1007/BF00229473

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