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In-vivo phosphorylation of the cardiac L-type calcium channel beta-subunit in response to catecholamines

  • Part I: Cardiac Development and Regulation
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Abstract

In canine myocardium, the β-subunit of the L-type Ca2+ channel is phosphorylated by cAMP dependent protein kinase in vitro as well as in vivo (Haase et al. FEBS Lett 335: 217–222, 1993). We have assessed the identity of the β-subunit as well as its in vivo phosphorylation in representative experimental groups of catecholamine-challenged canine hearts. Adrenergic stimulation by high doses of both noradrenaline and isoprenaline induced rapid (within 20 sec) and nearly complete phosphorylation of the Ca2+ channel β-subunit. Phosphorylation in vivo was about 4-fold higher as compared to untreated controls. When related to catecholamine-depleted (reserpine-treated) hearts noradrenaline and isoprenaline increased the in vivo phosphorylation of the β-subunit even 8-fold. This phosphorylation correlated positively with tissue levels of cAMP, endogenous particulated cAMP-dependent protein kinase (PKA) and the rate of contractile force development dP/dtmax. The results imply the involvement of a PKA-mediated phosphorylation of the Ca2+ channel β-subunit in the adrenergic stimulation of intact canine myocardium.

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Authors and Affiliations

  1. Cardiology Section, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, D-13122, Berlin-Buch, Germany

    Hannelore Haase, Sabine Bartel, Peter Karczewski, Ingo Morano & Ernst-Georg Krause

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  1. Hannelore Haase
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  2. Sabine Bartel
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  3. Peter Karczewski
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  4. Ingo Morano
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  5. Ernst-Georg Krause
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Haase, H., Bartel, S., Karczewski, P. et al. In-vivo phosphorylation of the cardiac L-type calcium channel beta-subunit in response to catecholamines. Mol Cell Biochem 163, 99–106 (1996). https://doi.org/10.1007/BF00408645

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