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Oxygen-dependent Leishmanicidal activity of stimulated macrophages

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Abstract

Peritoneal macrophages pretreated with different stimulants were analysed and compared with their respective controls for their ability to kill intracellular pathogenic L. donovani, (MHOM/IN/1983/AG83) an isolate from Indian subcontinent. Stimulation of macrophages by zymosan showed a higher microbicidal activity as compared to that by PMA. A correlation between microbicidal activity of the macrophages and the parameters related to respiratory burst activity such as liberation of 02 −, production of H202 and consumption of 02 was sought. All the parameters showed a decrease in case of infected macrophages in comparison to those of the non-infected ones. Thus, it is possible that the impairment of macrophage activation by intracellular Leishmania contributes to their survival in the toxic environment of the host.

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Abbreviations

02 :

superoxide anion

H202 :

hydrogen peroxide

PMA:

phorbol 12-myristate 13-acetate

PHPA:

phydroxylphenylacetic acid

Hepes:

N-2-hydroxyethyl piperazine-N-2-ethyl sulphonic acid

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Authors and Affiliations

  1. Leishmania Group, Biomembrane Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, 700032, Calcutta, India

    R. Chakraborty, S. Mukherjee & M. K. Basu (Director and Head Biomembrane Division)

Authors
  1. R. Chakraborty
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  2. S. Mukherjee
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  3. M. K. Basu
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Chakraborty, R., Mukherjee, S. & Basu, M.K. Oxygen-dependent Leishmanicidal activity of stimulated macrophages. Mol Cell Biochem 154, 23–29 (1996). https://doi.org/10.1007/BF00248457

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  • DOI: https://doi.org/10.1007/BF00248457

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