Abstract
The alteration of Ca2+-binding protein regucalcin mRNA expression in the kidney cortex of rats administered cisplatin and cephaloridine, which can induce kidney damage, was investigated. Cisplatin (0.25, 0.5 and 1.0 mg/100 g body weight) or cephaloridine (25, 50 and 100 mg/100 g) was intraperitoneally administered in rats, and 1, 2 and 3 days later they were sacrificed. The alteration in serum findings after the administration of cisplatin (1.0 mg/100 g) or cephaloridine (50 and 100 mg/100 g) demonstrated chemically induced kidney damage; blood urea nitrogen (BUN) concentration increased markedly and serum inorganic phosphorus or calcium concentration decreased significantly. Moreover, the administration of cisplatin (1.0 mg/100 g) or cephaloridine (100 mg/100 g) caused a remarkable increase of calcium content in the kidney cortex of rats, indicating kidney damage. The expression of regucalcin mRNA in the kidney cortex was markedly reduced by the administration of cisplatin or cephaloridine in rats, when the mRNA levels were analyzed by Northern blotting using rat liver regucalcin cDNA (0.9 kb). The mRNA decreases were seen with the used lowest dose of cisplatin or cephaloridine. The present study clearly demonstrates that the mRNA expression of Ca2+-binding protein regucalcin in the kidney cortex of rats is decreased by chemically induced kidney damage.
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Williamson JR, Cooper RH, Hoek JB: Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta 639: 243–295, 1981
Cheung WY: Calmodulin plays a pivotal role in cellular regulation. Science 202: 19–27, 1984
Nishizuka Y: Studies and perspectives of protein kinase C. Science 233: 305–312, 1986
Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43: 140–146, 1990
Yamaguchi M, Tai H: Inhibitory effect of calcium-binding protein regucalcin on Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase activity in rat liver cytosol. Mol Cell Biochem 106: 25–30, 1991
Yamaguchi M, Sakurai T: Inhibitory effect of calcium-binding protein regucalcin on Ca2+-activated DNA fragmentation in rat liver nuclei. FEBS Lett 279: 281–284, 1991
Yamaguchi M, Sakurai T: Reversible effect of calcium-binding protein regucalcin on the Ca2+-induced inhibition of deoxyuridine 5′-triphosphatase activity in rat liver cytosol. Mol Cell Biochem 110: 25–29, 1992
Shimokawa N, Yamaguchi M: Molecular cloning and sequencing of the cDNA coding for a calcium-binding regucalcin from rat liver. FEBS Lett 327: 251–255, 1993
Shimokawa N, Yamaguchi M: Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett 305: 151–154, 1992
Yamaguchi M, Isogai M: Tissue concentration of calcium-binding protein regucalcin in rats by enzyme-linked immuno-adsorbent assay. Mol Cell Biochem 122: 65–68, 1993
Shimokawa N, Yamaguchi M: Expression of hepatic calcium-binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver. FEBS Lett 316: 79–84, 1993
Yamaguchi M, Kurota H: Expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats: The stimulation by calcium administration. Mol Cell Biochem in press, 1995
Sausen PJ, Elfarra AA, Cooley AJ: Methimazole protection of rats against chemically induced kidney damageIn Vivo. J Pharmacol Exp Ther 260: 393–401, 1992
Taussky HH, Shon E: A microcolorimetric method for the deter mination of inorganic phosphorus. J Biol Chem 202: 675–685, 1953
Willis JB: Determination of calcium in blood serum by atomic absorption spectroscopy. Nature 186: 249–250, 1960
Yamaguchi M, Takei Y, Yamamoto T: Effect of thyrocalcitonin on calcium concentration in liver of intact and thyroparathyroidectomized rats. Endocrinology 96: 1004–1008, 1975
Elfarra AA, Jakobson I, Anders MW: Mechanism of S-(1,2 dichlorovinyl)glutathione induced nephrotoxicity. Biochem Pharmacol 35: 283–288, 1986
Bach PH, Lock EA: Nephrotoxicity,In Vitro toIn Vivo, Animals to Man, Plenum Press, New York, 1989
Tune BM, Fravert D, Hsu C-Y: Oxidative and mitochondrial toxic effects of cephalosporin antibiotics in the kidney. A comparative study of cephaloridine and cephaloglycin. Biochem Pharmacol 38: 795–802, 1989
Reed DJ: Glutathione: toxicological implications. Annu Rev Pharmacol Toxicol 30: 603–631, 1990
Montine TJ, Borch RF: Role of endogenous sulfur-containing nucleotides in anin vitro model of cis-diamminechloro platinum (II)-induced nephrotoxicity. Biochem Pharmacol 39: 1751–1757, 1990
Goldstein RS, Pasino DA, Hewitt WR, Hook JB: Biochemical mechanism of cephaloridine nephrotoxicity: Time and concent ration dependence of peroxidative injury. Toxicol Appl Pharmacol 83: 261–270, 1986
Takahashi H, Yamaguchi M: Activating effect of regucalcin on (Ca2+-Mg2+)-ATPase in rat liver plasma membranes: relation to sulfhydryl group. Mol Cell Biochem 136: 71–76, 1994
Yamaguchi M, Mori S: Activation of hepatic microsomal Ca2+-adenosine triphosphatase by calcium-binding protein regucalcin. Chem Pharm Bull (Tokyo) 37: 1031–1034, 1989
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Kurota, H., Yamaguchi, M. Suppressed expression of calcium-binding protein regucalcin mRNA in the renal cortex of rats with chemically induced kidney damage. Mol Cell Biochem 151, 55–60 (1995). https://doi.org/10.1007/BF01076896
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DOI: https://doi.org/10.1007/BF01076896