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Human cardiac myosin light chains: Sequence comparisons between myosin LC1 and LC2 from normal and idiopathic dilated cardiomyopathic hearts

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Abstract

The primary structures of light chains isolated from the human myocardium with idiopathic dilated cardiomyopathy (IDC) were determined and compared with the sequence structures of myosin light chains obtained from control human heart myosin. Sequences were determined by chemical analysis and the identity of N-terminal residues established by mass spectrometry. The N-terminal residues in essential (ELC) and regulatory (RLC) light chains were blocked and were identified to be trimethyl alanine. The amino acid sequences of ELC and RLC from control human myosin revealed a high degree of homology with those purified from rat and chicken cardiac myosin. Comparison with a published partial chemical sequence of the human heart myosin light chains revealed significant variations. However, there was very good agreement with published sequences obtained by molecular biological techniques. Sequences of the light chains from cardiomyopathic myosin revealed no difference in the primary structures when compared with control human heart myosin light chains indicating IDC had no influence on, nor was caused by, altered myosin light chain gene expression.

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Authors and Affiliations

  1. Rhône-Poulenc Rorer Central Research, 19406, King of Prussia, PA, USA

    John C. Holt

  2. Department of Pathology, University of Alabama, AL 35294, Birmingham, USA

    James B. Caulfield & Paul Norton

  3. Unit of Molecular and Cellular Biology, The Royal Veterinary College, University of London, London, UK

    Peter D. Chantler

  4. Dana-Farber Cancer Institute and Department of Cellular and Molecular Physiology, Harvard Medical School, 02115, Boston, MA, USA

    Henry S. Slayter

  5. Departments of Biochemistry and Orthopedic Research Laboratories, Montefiore Medical Center and Albert Einstein College of Medicine, 10467, Bronx, N.Y., USA

    Sarkis S. Margossian

  6. Division of Molecular and Cellular Medicine, Laboratory of Cardiac Biochemistry and Biophysics, Albany Medical College, 47 New Scotland Avenue, 12208, Albany, N.Y., USA

    Sarkis S. Margossian

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  1. John C. Holt
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  2. James B. Caulfield
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  3. Paul Norton
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  4. Peter D. Chantler
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  5. Henry S. Slayter
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  6. Sarkis S. Margossian
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Holt, J.C., Caulfield, J.B., Norton, P. et al. Human cardiac myosin light chains: Sequence comparisons between myosin LC1 and LC2 from normal and idiopathic dilated cardiomyopathic hearts. Mol Cell Biochem 145, 89–96 (1995). https://doi.org/10.1007/BF00925718

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  • DOI: https://doi.org/10.1007/BF00925718

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