Abstract
Hypoxia was reported to induce a decrease in phosphatidylcholine-hydrolyzing phospholipase activity (PC-PLA) in cultured rat cardiomyocytes. This work was intended to compare the influence of the presence of either eicosapentae noic acid (EPA) or docosahexaenoic acid (DHA) in the phospholipids on the PC-PLA activity in normoxic and hypoxic conditions. The enrichment of the medium with EPA or DHA resulted in cell phospholipids containing about 2% or 22% DHA, respectively. These cells were then submitted for 3.5 h to either normoxia or hypoxia and the PC-PLA activities were assayed using [1-14C] dioleoyl-PC (pH 8.4 for PC-PLA2 and 4.9 for PC-PLAT). The results show that both enzymic activities are significantly higher in DHA-rich cardiomyocytes. Hypoxia induced a significant decrease in PC-PLA2 (about 25%) which was not statistically different between the two groups of cells. The hypoxia-induced decrease in PC-PLA1 was not found significant. In conclusion, the nature of the long chain n-3 polyunsaturated fatty acids in the phospholipids appears to contribute to the regulation of PC-PLA activity but not to influence its decrease during hypoxia. (Mol Cell Biochem116: 75–78, 1992)
Similar content being viewed by others
References
Kinsella JE, Lokesch B, Stone RA: Dietary n-3 polyunsaturated fatty acids and amelioration of cardiovascular diseases. Am J Clin Nutr 52: 1–28, 1990
Charnock JS, Abeywardena M, McLennan PL: Comparative changes in the fatty acid composition of rat cardiac phospholipids after long term feeding of sunflower seed oil or tuna fish oil supplemented diet. Ann Nutr Metab 30: 393–406, 1986
Hartog JM, Verdouw PD, Klompe M, Lames JMJ: Dietary mackerel oil in pigs: effect on plasma lipids, cardiac sarcolemmal phospholipids and cardiovascular parameters: J Nutr 117: 1361–1378, 1988
Nalbone G, Leonardi J, Termine E, Portugal H, Lechene P, Pauli AM, Lafont H: Effects of fish oil, corn oil and lard on lipid peroxidation status and glutathione peroxidase activities in rat heart. Lipids 24: 179–186, 1989
Grynberg A, Fantini E, Athias P, Degois M, Guenot L, Courtois M, Khatami S: Modification of the n-6/n-3 fatty acid ratio in the phospholipids of rat ventricular myocytes in culture by the use of synthetic media: functional and biochemical consequences in normoxic and hypoxic conditions. J Mol Cell Cardiol 20: 863–874, 1988
Liautaud S, Grynberg A, Mourot J, Athias P: Contribution to the use of cultured cardiomyocytes as a model in nutritional studies: Fatty acid composition of the hearts of rats fed linseed oil or sunflower oil and cardiomyocytes grown on their sera. Cardioscience 2: 55–61, 1991 (Ed) World Review of Nutrition and Dietetics, Washington D.C., 1991, pp 278–291
Burr ML, Gilbert JF, Holliday RM, Elwood PC, Fehily AM, Rogers S, Sweetnam PM, Deadman NM: Effect of changes in fat, fish and fiber intakes on death and myocardial infarction: diet and reinfarction trial (DART). Lancet ii: 757–761, 1989
Van der Vusse GJ, van Bilsen M, Reneman RS: Is phospholipid degradation a critical event in ischemia-induced damage? NIPS 4: 49–53, 1989
Weglicki WB: Degradation of phospholipids in myocardial membranes. In: Wildenthal K (Ed) Degradative processes in heart and skeletal muscle, Elsevier, Amsterdam, 1980, pp 377–388
Hasen SL, Ford DA, Gross RW: Activation of a membraneassociated phospholipase A2 during myocardial ischemia which is highly selective for plasmalogen substrate. J Biol Chem 266: 5629–5633, 1991
Bentham JM, Higgins AJ, Woodward B: Phospholipase A2 activity during ischemia in the isolated rat heart. Basic Res Cardiol 82 (S1): 127–136, 1987
Schmertz D, Halverson J, Levitsky S: Differential alterations in phospholipase C and phospholipase A2 activity in ischemic rat heart. J Mol Cell Biol 21 (S2): S136, 1989
Grynberg A, Nalbone G, Degois M, Leonardi J, Athias P, Lafont H: Activities of some enzymes of phospholipid metabolism in cultured rat ventricular myocytes in normoxic and hypoxic conditions. Biochim Biophys Acta 958: 24–30, 1988
Nalbone G, Grynberg A, Chevalier A, Leonardi J, Termine E, Lafont H: Phospholipase A activity of cultured rat ventricular myocytes is affected by the nature of cellular polyunsaturated fatty acids. Lipids 25: 301–306, 1990
Grynberg A, Athias P, Degois M (1986): Effect of change in growth environment on culture myocardial cells investigated in a standardized medium. In vitro, 22: 44–50
Athias P, Grynberg A: Electrophysiological studies on heart cells in culture. In: ‘The Heart Cell in Culture’, Pinson A, Ed. CRC Press, Boca Raton, 1987, pp 125–158
Chevalier A, Demaison L, Grynberg A, Athias P: Influence of phospholipid polyunsaturated fatty acid composition on some metabolic disorders induced in rat cardiomyocytes by hypoxia and reoxygenation. J Mol Cell Cardiol 22: 1177–1186, 1990
Lepage C, Roy C: Direct transesterification of all classes of lipids in a one step resection. J Lip Res 27: 114–120, 1986
Folch J, Lees M, Sloane-Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 487–509, 1957
Juaneda P, Rocquelin G: Rapid and convenient separation of phospholipids from non phosphorus lipids in rat heart using silica cartridges. Lipids 20: 40–41, 1985
Lowry DH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1957
Mohammed BS, Hagve TA, Sprecher H: The metabolism of 20and 22-carbon unsaturated acids in rat heart and myocytes as mediated by feeding fish oil. Lipids 25: 854–858, 1990
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Grynberg, A., Nalbone, G., Leonardi, J. et al. Eicosapentaenoic and docosahexaenoic acids in cultured rat ventricular myocytes and hypoxia-induced alterations of phospholipase—A activity. Mol Cell Biochem 116, 75–78 (1992). https://doi.org/10.1007/BF01270572
Issue Date:
DOI: https://doi.org/10.1007/BF01270572