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RNA-mediated transfer of cellular immunity to a synthetic env antigen of the human immunodeficiency virus (HIV-1)

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Abstract

A sheep was immunized with a synthetic peptide corresponding to amino acid residues 586–606 of the precursor envelope protein GP-160 of the HIV-1 including a conserved epitope region of the GP-41 transmembrane protein in the mature viral particles, referred to as SM 284 HIV-1 [1]. It is demonstrated that immune RNA extracted from the lymphoid organs of the immunized animal (SM 284 HIV-1 I-RNA) was able to transfer immune cellular reactivity to SM 284 HIV-1 in vitro to human and rabbit lymphocytes and in vivo to Cebus apella monkeys. The transfer was detected by the leukocyte adherence inhibition test (LAI) as an indicator of cellular reactivity. One of the most relevant results was the demonstration that SM 284 HIV-1 I-RNA was able to induce cellular immunological memory in vivo in monkeys. These results may be relevant to delineate a new alternative for immunomodulation against HIV infection.

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Abbreviations

A:

Absorbance

ATA:

Aurintricarboxylic acid

cDNA:

Complementary DNA

DNase:

Deoxyribonuclease

env:

Envelope

ELISA:

Enzyme Linked Immunosorbent Assay

MEM:

Minimum Essential Medium

gp:

Glycoprotein

HBV:

Hepatitis B Virus

HIV:

Human Immunodeficiency Virus

LAI:

Leukocyte Adherence Inhibition

RNase:

Ribonuclease

PK:

Proteinase K

SDS:

Sodium Dodecyl Sulfate

References

  1. Wang JJG, Steel S, Wisniewolski R, Wang CY: Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp 41 envelope protein. Proc Natl Acad Sci USA 83: 6159–6183, 1986

    Google Scholar 

  2. Dalgleish AG, Beverley PCL, Clapham PR, Crawfor DH, Greaves MF, Weiss RA: The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 312: 763–767, 1984

    Google Scholar 

  3. Klatzman D, Champagne E, Chamaret S, Gruest J, Guetard, Hercent T, Gluckman JC, Montagnier L: T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature 312: 767–768, 1984

    Google Scholar 

  4. Wahren B, Morfeldt-Mansson L, Biberfeld G, Morberg L, Ljungman P, Nordlund S, Bredeberg-Radén U, Werner A, Lower J, Kurt R: Impaired specific cellular response to HTLV-III before other immune defects in patients with HTLV-III infection. New Engl J Med 315: 393–394, 1986

    Google Scholar 

  5. Weiss RA, Claphan PR, Ceingsong-Popov R: Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients. Nature 316: 69–72, 1985

    Google Scholar 

  6. Popovic M, Sarngadharan MG, Read E, Gallo R: Detection, isolation and continuous production of cytopathic retro-virus (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497–500, 1984

    Google Scholar 

  7. Plata F, Autran B, Martins LP, Wain-Hobson S, Raphael M, Mayaud C, Denis M, Guillon JM, Debré P: AIDS - virus specific cytotoxic T lymphocytes in lung disorders. Nature 328: 348–351, 1987

    Google Scholar 

  8. Walker BD, Chakrabarti S, Moss B, Paradis TJ, Flynn T, Durno AG, Blunberg RS, Kaplan J, Hirsch MS, Schooleys RT: HIV-specific cytotoxic T lymphocytes in seropositive individuals: Nature 328: 345–347, 1987

    Google Scholar 

  9. Walker BD, Flexner C, Paradis TJ, Fuller TC, Hirsch MS, Schooley R, Moss B: HIV-1 reverse transcriptase is a target for cytotoxic T lymphocytes in infected individuals: Science 240:64–66, 1988

    Google Scholar 

  10. Walker CM, Moody DJ, Stiles DP, Levy JA: CD8+ lymphocytes can control HIV infection in vitro by suppression virus replication. Science 234: 1563–1566, 1986

    Google Scholar 

  11. Alter HJ, Eichberg JW, Masur H, Saxinger W, Macher AM, Lane HC, Fauci AS: Transmission of HTLV-III infection from human plasma to chimpanzees. An animal model for AIDS. Science 226: 549–552, 1984

    Google Scholar 

  12. Francis DP, Feorino PM, Braderson JR, McClure HM, Getchel JP, McGrath CR, Swenson B, McDougal JS, Palmer EL, Harrison AK, Barré-Sinoussi F, Chermam JC, Montagnier L, Curran JW, Cabradilla CD, Kalyanamaran VS: Infection of chimpanzees with lymphadenopathy associated virus. Lancet 2: 1276–1277, 1984

    Google Scholar 

  13. Gajdusek C, Gibbs Jr CJ, Roberts-Johnson P, Amyx HL, Asher DM, Epstein LG, Sarin PS, Malvish A, Gallo RC, Arthur L, Montagnier L, Mildvan D: Infection of chimpanzees by human T-lymphotropic retroviruses in brain and other tissues from AIDS patients. Lancet 1: 55–56, 1985

    Google Scholar 

  14. Claverie JM, Kourilsky P, Langlade-Demoyen P, Chalufour-Prochnicka A, Dadaglio G, Tekaia F, Plata F, Bougueleret L: T-immunogenic peptides are constituted of rare sequence patterns. Use in the identification of T epitopes in the human immunodeficiency virus gag protein Eur J Immunol 18: 1547–1553, 1988

    Google Scholar 

  15. Michel F, Hoffenbach A, Langlade-Demoyen P, Guy B, Girard M, Lecocq JP, Wain-Hobson S, Kieny MP, Plata F: HIV-specific T lymphocytes immunity in mice immunized with a recombinant vaccinia virus. Eur J Immunol 18: 1917–1924, 1988

    Google Scholar 

  16. Mendes NF, Peixinho ZF, Longo IM, Moura NC, Beck C, Passos Jr GAS, De Lucca FL: Transfer of cell-mediated immunity to HIV antigens in vivo to a Cebus monkey and in vitro to human lymphocytes with xenogeneic immune RNA. Braz J Med Biol Res 20: 747–751, 1986

    Google Scholar 

  17. Passos Jr GAS: Transferência in vitro e in vivo de imunidade celular a um peptídeo sintético contendo um dos epítopos da glicoproteína 41 do vírus da imunodeficiência humana — 1 (HIV-1) com RNA imune. Doctoral Thesis, School of Medicine of Ribeirão Preto, University of São Paulo pp. 1–156, 1988

  18. De Lucca FL, Bertolini MC, Zini MN: In vitro transfer of reactivity to Trypanosoma cruzi antigens from rat cells to human cells with immune RNA. J Infect Dis 145: 148–151, 1982

    Google Scholar 

  19. Passos Jr GAS, De Lucca FL: In vivo induction of immunological memory to human tumor extract with poly (A) containing immune RNA. Cell Mol Biol 34: 157–164, 1988

    Google Scholar 

  20. Steele Jr G, Wang BS, Mannick JA: Immune RNA and transfer factor. In: Mihich E, (editor). Imunological approaches to cancer therapeutics. New York, USA: John Wiley and Sons, Inc., 1982, 257–275

    Google Scholar 

  21. Artigas C, Thomson DMP, Durno M, Sutherland M, Scanzano R, Shenouda G, Dubois AEJ: Identification of Mr 40,000 polypeptide from colorectal cancer with expresses organ-specific cancer neoantigen activity as determined by leukocyte adherence inhibition. Cancer Res 46: 1874–1881, 1986

    Google Scholar 

  22. Grossner N, Thomson DMP: Cell-mediated antitumor immunity in breast cancer patients evaluated by antigen-induced leukocyte adherence inhibition in test tubes. Cancer Res 35: 2571–2579, 1975

    Google Scholar 

  23. Merrifield RB: Solid phase peptide synthesis I. The synthesis of a tetrapeptide. J Am Chem Soc 85: 2149–2154, 1963

    CAS  Google Scholar 

  24. White BN, De Lucca FL: Preparation and analysis of RNA. In: Turner RB, (editor). Analytical Biochemistry of Insects. Amsterdam, Holand: Elsevier-Scientific, 1977, 85–130

    Google Scholar 

  25. Matews MB: Mammalian chain initiation: the effect of aurin tricarboxylic acid. FEBS Letters 15: 201–204, 1971

    Google Scholar 

  26. Mackie CM, Buxton DB, Mowbray J: Aurin tricarboxylic acid as both a stimulator and an inhibitor of cell-free protein synthesis by heart polyribosomes. Biochem Biophys Res Comm 144: 750–757, 1982

    Google Scholar 

  27. Kuwano H, Scott M, Young JF, Ennis FA: HA2 Subunit of influenza H1 and H2 subtype viruses induces a protective cross-reactive cytotoxic T-lymphocyte response. J Immunol 140:1264–1268, 1988

    Google Scholar 

  28. Taylor PM, Asconas BA: Influenza nucleoprotein specific T-cell clones are protective in vivo. Immunol 58: 417–420, 1986

    Google Scholar 

  29. Shi-Shan L, Chang Fu L, Fu-Ying H, Chang-Xue Z: Preparation and clinical use of HBsAG immune RNA. Lancet 1: 197–198

  30. Viza D, Boucheix C, Kern DH, Pilch Y: Human lymphoblastoid cells in culture replicate immune information carried by xenogeneic RNA. Differentiation 11: 181–184, 1978

    Google Scholar 

  31. Zucchi TMAD, Passos Jr GAS, De Lucca FL: RNA-mediated genetic transformation in Aspergillus nidulans. Cell Mol Biol 35: 573–580, 1989

    Google Scholar 

  32. Niu MC, Xue GX, Niu LC, Huang HZ: Transfer of information from mRNA to chromosomes by reverse transcription in early development of goldfish eggs. Cell Mol Biol 35: 333–345.

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Authors and Affiliations

  1. Lab. of Genetics, Dept. of Morphology, School of Dentistry of Ribeirão Preto, University of São Paulo, Via do Café s/n, 14049, Ribeirão Preto, SP, Brasil

    Geraldo A. S. Passos Jr

  2. Dept. of Biochemistry, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brasil

    Fernando L. de Lucca

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  1. Geraldo A. S. Passos Jr
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  2. Fernando L. de Lucca
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Passos, G.A.S., de Lucca, F.L. RNA-mediated transfer of cellular immunity to a synthetic env antigen of the human immunodeficiency virus (HIV-1). Mol Cell Biochem 108, 1–8 (1991). https://doi.org/10.1007/BF00239536

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  • DOI: https://doi.org/10.1007/BF00239536

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