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Structural aspects of the cytochromeb 6 f complex; structure of the lumen-side domain of cytochromef

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Abstract

The following findings concerning the structure of the cytochromeb 6 f complex and its component polypeptides, cytb 6, subunit IV and cytochromef subunit are discussed:

  1. (1)

    Comparison of the amino acid sequences of 13 and 16 cytochromeb 6 and subunit IV polypeptides, respectively, led to (a) reconsideration of the helix lengths and probable interface regions, (b) identification of two likely surface-seeking helices in cytb 6 and one in SU IV, and (c) documentation of a high degree of sequence invariance compared to the mitochondrial cytochrome. The extent of identity is particularly high (88% for conserved and pseudoconserved residues) in the segments of cytb 6 predicted to be extrinsic on then-side of the membrane.

  2. (2)

    The intramembrane attractive forces betweentrans-membrane helices that normally stabilize the packing of integral membrane proteins are relatively weak.

  3. (3)

    The complex isolated in dimeric form has been visualized, along with isolated monomer, by electron microscopy. The isolated dimer is much more active than the monomer, is the major form of the complex isolated and purified from chloroplasts, and is inferred to be a functional form in the membrane.

  4. (4)

    The isolated cytb 6 f complex contains one molecule of chlorophylla.

  5. (5)

    The structure of the 252 residue lumen-side domain of cytochromef isolated from turnip chloroplasts has been solved by X-ray diffraction analysis to a resolution of 2.3 Å.

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References

  • Bald, D., Kruip, J., Boekema, E. J., and Rögner, M. (1992).Research in Photosynthesis (Murata, N., ed.), Kluwer, Dordrecht, Vol. I, pp. 629–632.

    Google Scholar 

  • Beattie, D. S. (1993).J. Bioenerg. Biomembr. 25, 233–244.

    Google Scholar 

  • Bechmann, G., Weiss, H., and Rich, P. R. (1992).Eur. J. Biochem. 208, 315–325.

    Google Scholar 

  • Bergstrom, J., and T. Vanngard (1982).Biochim. Biophys. Acta 682, 452–456.

    Google Scholar 

  • Bormann, B. J., and Engelman, D. E. (1992).Annu. Rev. Biophys. Biophys. Chem. 19, 369–403.

    Google Scholar 

  • Brand, S. N., Tan, X., and Widger, W. R. (1992).Plant Mol. Biol. 20, 481–491.

    Google Scholar 

  • Breyton, C., de Vitry, C., and Popot, J.-L. (1994)J. Biol. Chem., in press.

  • Büschlen, S., Choquet, Y., Kuras, R., and Wollman, F. A. (1991).FEBS Lett. 284, 257–262.

    Google Scholar 

  • Canals, F. (1992).Biochemistry 31, 4493–4501.

    Google Scholar 

  • Chain, R., and Malkin, R. (1991).Photosynth. Res. 28, 59–68.

    Google Scholar 

  • Cote, J.-C., Wu, N.-H., and Wu, R. (1988).Plant Mol. Biol. 11, 873–874.

    Google Scholar 

  • Cramer, W. A., Black, M. T., Widger, W. R., and Girvin, M. E. (1987).The Light Reactions (Barber, J., ed.), Elsevier, Amsterdam, pp. 446–493.

    Google Scholar 

  • Cramer, W. A., Furbacher, P. N., Szczepaniak, A., and Tae, G.-S. (1991).Current Topics in Bioenergetics, Vol. 16 (Lee, C. P., ed.), Academic Press, Orlando, pp. 179–222.

    Google Scholar 

  • Cramer, W. A., Engelman, D. M., von Heijne, G., and Rees, D. C. (1992).FASEB J. 6, 3397–3402.

    Google Scholar 

  • Crofts, A. R. (1985).The Enzymes of Biological Membranes (Martinosi, A., ed.), 2nd edn, Vol. 4, Plenum Press, New York, pp. 347–382.

    Google Scholar 

  • Crofts, A. R., Robinson, H., Andrews, K., Van Doren, S., and Berry, E. (1987).Cytochrome Systems: Molecular Biology and Bioenergetics (Papa, S., Chance, B., and Ernster, L., eds.), Plenum Press, New York, pp. 617–624.

    Google Scholar 

  • Crofts, A. R., Wang, Z., Chen, Y., Mahalingam, S., Yun, C. H., and Gennis, R. B. (1990).Highlights in Ubiquinone Research (Lenaz, G., ed.), Taylor and Francis, London, pp. 98–103.

    Google Scholar 

  • Crowder, M. S., Prince, R. C., and Bearden, A. (1982).FEBS Lett. 144, 204–208.

    Google Scholar 

  • Daldal, F., Tokito, M. K., Davidson, E., and Faham, M. (1989).EMBO J. 8, 3951–3961.

    Google Scholar 

  • Davis, D. J., Frame, M. K., and Johnson, D. A. (1988).Biochim. Biophys. Acta 936, 61–66.

    Google Scholar 

  • Degli Esposti, M., DeVries, S., Crimi, M., Ghelli, A., Patarnello, T., and Meyer, A. (1993).Biochim. Biophys. Acta 1143, 243–271.

    Google Scholar 

  • Deisenhofer, J., and Michel, H. (1989).EMBO J. 8, 2149–2169.

    Google Scholar 

  • de Vries, S., Albracht, S. P. J., Berden, J. A., Marres, C. A. M., and Slater, E. C. (1983).Biochim. Biophys. Acta 723, 91–103.

    Google Scholar 

  • diRago, J. P., and Colson, A.-M. (1988).J. Biol. Chem. 263, 12564–12570.

    Google Scholar 

  • Eisenberg, D. (1984).Annu. Rev. Biochem. 53, 595–623.

    Google Scholar 

  • Fukuzawa, H., Yoshida, T., Kohchi, T., Okumura, T., Sawano, Y., and Ohyama, K. (1987).FEBS Lett. 220, 61–66.

    Google Scholar 

  • Furbacher, P. N., Girvin, M. E., and Cramer, W. A. (1989).Biochemistry 28, 8990–8998.

    Google Scholar 

  • Gal, A., Mets, L. J., and Ohad, I. (1990a).Current Research in Photosynthesis (Baltscheffsky, M., ed.), Vol. II, Kluwer Academic, Dordrecht, pp. 779–781.

    Google Scholar 

  • Gal, A., Mor, T. S., Hauska, G., Herrmann, R., and Ohad, I. (1990b).Current Research in Photosynthesis (Baltscheffsky, M., ed.), Vol. II, Kluwer Academic, Dordrecht, pp. 783–785.

    Google Scholar 

  • Gavel, Y., Steppuhn, J., Herrmann, R., and von Heijne, G. (1991).FEBS Lett. 282, 41–46.

    Google Scholar 

  • Gennis, R. B., Barquera, B., Hacker, B., Van Doren, S. R., Arnaud, S., Crofts, A. R., Davidson, E., Gray, K. R., and Daldal, F. (1993).J. Bioenerg. Biomembr. 25, 195–209.

    Google Scholar 

  • Girvin, M. E., and Cramer, W. A. (1984).Biochim. Biophys. Acta 767, 29–38.

    Google Scholar 

  • Glaser, E. A., and Crofts, A. R. (1984).Biochim. Biophys. Acta 766, 322–333.

    Google Scholar 

  • Gonzalez-Halphen, D., Lindorfer, M. A., and Capaldi, R. A. (1988).J. Biol. Chem. 27, 7021–7031.

    Google Scholar 

  • Graham, L. A., Brandt, U., Sargent, J. S., and Trumpower, B. L. (1993).J. Bioenerg. Biomembr. 25, 245–257.

    Google Scholar 

  • Grasberger, B., Minton, A. P., DeLisi, C., and Metzger, H. (1986).Proc. Natl. Acad. Sci. USA 83, 6258–6262.

    Google Scholar 

  • Gray, J. C. (1978).Eur. J. Biochem. 82, 133–141.

    Google Scholar 

  • Gray, J. C. (1992).Photosynth. Res. 34, 359–374.

    Google Scholar 

  • Gray, K. A., and Daldal, F. (1993).Biophys. J. 64, 105a.

    Google Scholar 

  • Greer, K. L., and Golden, S. S. (1992).Plant Mol. Biol. 19, 355–365.

    Google Scholar 

  • Gross, E. L. (1993).Photosyn. Res. 37, 103–116.

    Google Scholar 

  • Hacker, B., Barquera, B., Crofts, A. R., and Gennis, R. B. (1993).Biophys. J. 64, 105a.

    Google Scholar 

  • Haley, J., and Bogorad, L. (1989).Proc. Natl. Acad. Sci. USA 86, 1534–1538.

    Google Scholar 

  • Hauska, G., Hurt, E., Gabellini, N., and Lockau, W. (1983).Biochim. Biophys. Acta 726, 97–133.

    Google Scholar 

  • Hauska, G., Nitschke, W., and Herrmann, R. G. (1988).J. Bioenerg. Biomem. 20, 211–228.

    Google Scholar 

  • Heinemeyer, W., Alt, J., and Herrmann, R. G. (1984).Curr. Genet. 8, 543–549.

    Google Scholar 

  • Hiratsuka, J., Shimada, H., Whittier, R., Ishibashi, T., Sakamoto, M., Mori, M., Kondo, C., Honji, Y., Sun, C. R., Meng, B. Y., Li, Y. Q., Kanno, A., Nishizawa, Y., Hirai, A., Shinozaki, K., and Sugiura, M. (1989).Mol. Gen. Genet. 217, 185–194.

    Google Scholar 

  • Hird, S. M., Webber, A. N., Wilson, R. J., Dyer, T. A., and Gray, J. C. (1991).Plant Mol. Biol. 16, 745–747.

    Google Scholar 

  • Ho, K. K., and Krogmann, D. W. (1980).J. Biol. Chem. 255, 3855–3861.

    Google Scholar 

  • Hope, A. B. (1993).Biochim. Biophys. Acta 1143, 1–22.

    Google Scholar 

  • Howell, N., and Gilbert, K. (1988).J. Mol. Biol. 203, 607–618.

    Google Scholar 

  • Huang, D. H., Everly, M., Cheng, R. H., Heymann, J. B., Schägger, H., Baker, T. S., and Cramer, W. A. (1993). Submitted.

  • Hurt, E. C., and Hauska, G. (1983).FEBS Lett. 153, 413–419.

    Google Scholar 

  • Jacobs, R. E., and White, S. H. (1989).Biochemistry 8, 3421–3437.

    Google Scholar 

  • Johnson, E. M., Schabelrauch, L. S., and Sears, B. B. (1991).Mol. Gen. Genet. 225, 106–112.

    Google Scholar 

  • Joliot, P., and Joliot, A. (1988).Biochim. Biophys. Acta 933, 319–333.

    Google Scholar 

  • Kallas, T., Spiller, S., and Malkin, R. (1988).J. Biol. Chem. 263, 14334–14342.

    Google Scholar 

  • Kirwin, P. M., Elderfield, P. D., Williams, R. S., and Robinson, C. (1991).J. Biol. Chem. 263, 18128–18132.

    Google Scholar 

  • Kramer, D. M., and Crofts, A. R. (1990).Curr. Res. Photosyn. (Baltscheffksy, M., ed.), Vol. III, Kluwer Academic, Dordrecht, pp. 283–286.

    Google Scholar 

  • Kramer, D. M., and Crofts, A. R. (1992).Res. Photosyn. (N. Murata, ed.) II, pp. 491–494, Kluwer, Dordrecht.

    Google Scholar 

  • Kück, U. (1989).Mol. Gen. Genet. 218, 257–265.

    Google Scholar 

  • Kutoh, E., and Sone, N. (1988).J. Biol. Chem. 263, 9020–9026.

    Google Scholar 

  • Lemmon, M. A., Flanagan, J. M., Hunt, J. F., Adair, B. D., Bormann, B. J., and Engelman, D. E. (1992).J. Biol. Chem. 267, 7683–7689.

    Google Scholar 

  • Leonard, K., Wingfield, P., Arod, T., and Weiss, H. (1981).J. Mol. Biol. 149, 259–274.

    Google Scholar 

  • Li, L.-B., Zou, Y.-P., Yu, L., and Yu, C.-A. (1991).Biochim. Biophys. Acta 1057, 215–222.

    Google Scholar 

  • Link, T. A., Haase, U., Brandt, U., and von Jagow, G. (1993).J. Bioenerg. Biomembr. 25, 221–232.

    Google Scholar 

  • Martinez, S. E., Szczepaniak, A., Smith, J. L., and Cramer, W. A. (1991).Biophys. J. 59, 524a.

    Google Scholar 

  • Martinez, S. E., Smith, J. L., Huang, D., Szczepaniak, A., and Cramer, W. A. (1992).Research in Photosynthesis (Murata, N., ed.), Vol. II, Kluwer Academic, Dordrecht, pp. 495–498.

    Google Scholar 

  • Martinez, S. E., Huang, D., Szczepaniak, A., Cramer, W. A., and Smith, J. L. (1993). Manuscript in preparation.

  • Milburn, M. V., Prive, G. G., Milligan, D. L., Scott, W. G., Yeh, J., Jancarik, J., Koshland, D. E., Jr., and Kim, S. H. (1991).Science 254, 1342–1347.

    Google Scholar 

  • Morand, L., Frame, M. K., Colvert, K. K., Johnson, D. A., Krogmann, D. W., and Davis, D. J. (1989).Biochemistry 28, 8039–8047.

    Google Scholar 

  • Mörschel, E., and Staehelin, A. (1983).J. Cell Biol. 97, 301–310.

    Google Scholar 

  • Nalecz, M. J., and Azzi, A. (1985).Arch. Biochem. Biophys. 240, 921–931.

    Google Scholar 

  • Nieboer, P., and Berden, J. A. (1992).Biochim. Biophys. Acta 1101, 90–96.

    Google Scholar 

  • Nitschke, W., Hauska, G., and Crofts, A. R. (1988).FEBS Lett. 232, 204–208.

    Google Scholar 

  • Ohnishi, T., Schägger, H., Meinhardt, S. W., LoBrutto, R., Link, T. A., and von Jagow, G. (1989).J. Biol. Chem. 264, 735–744.

    Google Scholar 

  • Osiewacz, H. D. (1992).Arch. Microbiol. 157, 336–342.

    Google Scholar 

  • Pakula, A., and Simon, M. (1992).Nature 355, 496–497.

    Google Scholar 

  • Pfanner, N., and Neupert, W. (1990).Annu. Rev. Biochem. 59, 331–353.

    Google Scholar 

  • Phillips, A. C., and Gray, J. C. (1984).Mol. Gen. Genet. 194, 477–484.

    Google Scholar 

  • Piccioni, R., Bellemare, G., and Chua, N.-H. (1982).Methods in Chloroplast Molecular Biology (Edelman, M.et al., eds.), Chap. 80, pp. 985–1015.

  • Popot, J.-L., and Engelman, D. E. (1990).Biochemistry 29, 4031–4039.

    Google Scholar 

  • Qin, L., and Kostic, N. M. (1993).Biochemistry 32, 6073–6080.

    Google Scholar 

  • Redinbo, M. R., Yeates, T. O., and Merchant, S. (1994).J. Bioenerg. Biomem., in press.

  • Reimann, A., and Kueck, U. (1989).Plant Mol. Biol. 13, 255–256.

    Google Scholar 

  • Reverdatto, S. V., Andreeva, A. V., Buryakova, A. A., Chakhmakhcheva, O. G., and Efimov, V. A. (1989).Nucleic Acids Res. 17, 2859–2860.

    Google Scholar 

  • Rich, P. R., Madgwick, S. A., and Moss, D. A. (1991).Biochim. Biophys. Acta 1058, 312–328.

    Google Scholar 

  • Rich, P., Madgwick, S. A., Brown, S., von Jagow, G., and Brandt, U. (1992).Photosynth. Res. 34, 465–477.

    Google Scholar 

  • Riedel, A., Rutherford, W., Hauska, G., Müller, A., and Nitschke, W. (1991).J. Biol. Chem. 266, 17838–17844.

    Google Scholar 

  • Rigby, S. E. J., Moore, G. R., Gray, J. C., Godsby, P. M. A., George, S. J., and Thomson, A. J. (1988).Biochem. J. 256, 571–577.

    Google Scholar 

  • Robertson, D. E., and Dutton, P. L. (1988).Biochim. Biophys. Acta 935, 273–291.

    Google Scholar 

  • Robertson, D. E., Daldal, F., and Dutton, P. L. (1990).Biochemistry 29, 11249–11260.

    Google Scholar 

  • Rock, C. D., Barkan, A., and Taylor, W. C. (1987).Curr. Genet. 12, 69–77.

    Google Scholar 

  • Saraste, M. (1984).FEBS Lett. 166, 367–372.

    Google Scholar 

  • Schägger, H., and von Jagow, G. (1991).Anal. Biochem. 199, 223–231.

    Google Scholar 

  • Schlunegger, B., and Stutz, E. (1984).Curr. Genet. 8, 629–634.

    Google Scholar 

  • Schmitt, M. E., and Trumpower, B. L. (1990).J. Biol. Chem. 265, 17005–17011.

    Google Scholar 

  • Selak, M. A., and Whitmarsh, J. (1982).FEBS Lett. 150, 286–292.

    Google Scholar 

  • Shinozaki, K., Ohme, M., Tanaka, M., Wakasugi, T., Hayashida, N., Matsubayashi, T., Zaita, N., Chunwongse, J., Obokata, J., Yamaguchi-Shinozaki, K., Ohto, C., Torazawa, K., Meng, B. Y., Sugita, M., Deno, H., Kamogashira, T., Yamada, K., Kusuda, J., Takaiwa, F., Kato, A., Tohdoh, N., Shimda, H., and Sugiura, M. (1986).EMBO J. 5, 2043–2049.

    Google Scholar 

  • Shiver, J. W., Peterson, A. A., Widger, W. R., and Cramer, W. A. (1989).Methods Enzymol. 172, 439–461.

    Google Scholar 

  • Siedow, J. N., Vickery, L. E., and Palmer, G. (1980).Arch. Biochem. Biophys. 203, 101–107.

    Google Scholar 

  • Simpkin, D., Palmer, G., Devlin, F. J., McKenna, M. C., Jensen, G. M., and Stephens, P. J. (1989).Biochemistry 28, 8033–8039.

    Google Scholar 

  • Szczepaniak, A., and Cramer, W. A. (1990).J. Biol. Chem. 265, 17720–17726.

    Google Scholar 

  • Szczepaniak, A. D., Huang, D., Keenan, T. W., and Cramer, W. A. (1991).EMBO J. 10, 2757–2764.

    Google Scholar 

  • Thony-Meyer, L., James, P., and Hennecke, H. (1991).Proc. Natl Acad. Sci. USA,88, 5001–5005.

    Google Scholar 

  • Trumpower, B. L. (1990).J. Biol. Chem. 265, 11409–11412.

    Google Scholar 

  • Turmel, M., Boulanger, J., and Bergeron, A. (1989).Nucleic Acids Res. 17, 3593–3593.

    Google Scholar 

  • Verner, K., and Schatz, G. (1988).Science 241, 1307–1313.

    Google Scholar 

  • Von Jagow, G., and Sebald, W. (1980).Ann. Rev. Biochem. 49, 281–314.

    Google Scholar 

  • Von Heijne, G. (1992).J. Mol. Biol. 225, 487–494.

    Google Scholar 

  • Weiss, H., and Kolb, H. J. (1979).Eur. J. Biochem. 99, 139–149.

    Google Scholar 

  • Widger, W. R., Cramer, W. A., Herrmann, R., and Trebst, A. (1984).Proc. Natl. Acad. Sci. USA 81, 674–678.

    Google Scholar 

  • Widger, W. R., and Cramer, W. A. (1991).Cell Culture and Somatic Cell Genetics of Plants: The Molecular Biology of Plastids and the Photosynthetic Apparatus (Vasil, I. K., and Bogorad, L., eds.), Academic Press, Orlando, pp. 149–176.

    Google Scholar 

  • Willey, D. L., Auffret, A. D., and Gray, J. C. (1984).Cell 36, 555–562.

    Google Scholar 

  • Yang, M., and Trumpower, B. L. (1993).J. Biol. Chem., in press.

  • Yun, C.-H., Van Doren, S. R., Crofts, A. R., and Gennis, R. B. (1991).J. Biol. Chem.,266, 10967–10973.

    Google Scholar 

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  1. Department of Biological Sciences, Purdue University, 47907, West Lafayette, Indiana

    W. A. Cramer, S. E. Martinez, D. Huang, G. -S. Tae, R. M. Everly, J. B. Heymann, R. H. Cheng, T. S. Baker & J. L. Smith

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Cramer, W.A., Martinez, S.E., Huang, D. et al. Structural aspects of the cytochromeb 6 f complex; structure of the lumen-side domain of cytochromef . J Bioenerg Biomembr 26, 31–47 (1994). https://doi.org/10.1007/BF00763218

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