Abstract
Fluorescence resonance energy transfer (FRET) from NBD-PE to rhodamine-PE has been used to measure the average area occupied by surfactant molecules in lipid bilayers. The Foerster radius has been determined from the spectral overlap of donor fluorescence and acceptor absorption (R 0=4.6 nm). The results of steady-state as well as of time-resolved investigations have been compared. The analysis of time-resolved fluorescence data by means of nonexponential decay functions yields an average area per lipid of 0.65 nm2 in pure POPC bilayers. The area per surfactant in two-component C12E n /POPC-membranes (n=2, 4, 6) has been determined and compared with the results of X-ray investigations. The surfactant head group seems to adapt a predominantly disordered confirmation within the bilayer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. Ollivon, O. Eidelman, R. Blumenthal, and A. Walter (1988)Biochemistry 27, 1695–1703.
J.-L. Rigaud, M.-T. Paternostre, and A. Bluzat (1988)Biochemistry 27, 2677–2688.
D. A. Haugen and M. J. Coon (1976)J. Biol. Chem. 251, 7929–7939.
R. P. Rand and V. A. Persegian (1989)Biochim. Biophys. Acta 988, 351–376.
G. Cevc and D. Marsh (1987)Phospholipid Bilayers: Physical Principles and Models, Wiley, New York.
J. N. Israelachvili and H. Wennerström (1991)J. Phys. Chem. 96, 520–531.
D. Needham, T. McIntosh, and D. Lasic (1992)Biochim. Biophy. Acta 1108, 40–48.
F. Volke, S. Eisenblätter, J. Galle, and G. Klose (1994)Chem. Phys. Lipids (in press).
C. Tanford, Y. Nozaki, and M. F. Rohde (1977)J. Phys. Chem. 81, 1555–1563.
M. Rösch (1972)Tenside 9, 23–31.
M. J. Schick (1962)J. Coll. Sci. 17, 801.
M. J. Hope, M. B. Bally, G. Webb, and P. R. Cullis (1985)Biochim. Biophys. Acta 812, 55–65.
K. Kinosita, Jr., S. Kawato, and A. Ikegami (1977)Biophys. J. 20, 289–305.
H. Binder, G. Lantzsch, and K. Dittes (1989)Wiss. Z. Karl-Marx-Univ. Math.-nat. wiss. Reihe 38(6), 569–578.
F. Jähnig (1979)Proc. Natl. Acad. Sci. USA 76, 6361–6365.
P. K. Wolber and B. S. Hudson (1979)Biophys. J. 28, 197–210.
A. S. Waggoner and L. Stryer (1970)Proc. Natl. Acad. Sci. USA 67, 579–585.
A. Chattopadhyay and E. London (1987)Biochemistry 26, 39–44.
H. Heerklotz, H. Binder, and G. Lantzsch (1994).J. of Fluorescence 4, 349–352.
B. König (1992) thesis, University of Leipzig, Leipzig.
G. Klose, B. König, H. W. Meyer, G. Schulze, and G. Degovics (1988)Chem. Phys. Lipids 47, 225–234.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lantzsch, G., Binder, H. & Heerklotz, H. Surface area per molecule in lipid/C12E n membranes as seen by fluorescence resonance energy transfer. J Fluoresc 4, 339–343 (1994). https://doi.org/10.1007/BF01881452
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01881452