Abstract
Streptavidin and avidin were adsorbed onto graphite surfaces and images of their structures were obtained using a scanning tunnelling microscope. Individual protein molecules and their subunits were observed, and their sizes were found to agree closely with measurements from a subunit model based upon the crystal structure of streptavidin. The process of adsorption of streptavidin-FITC and avidin succinylfluorescein isothiocyanate onto the graphite surface was also investigated using fluorescence microscopy.
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L.Chaiet and F.Wolf, Arch. Biochem. Biopys., 106 (1964) 1
E. P.Diamandis and T. K.Christopoulos, Clinical Chem., 37 (1991) 625.
H.Morgan, and D. M.Taylor, Biosensors & Bioelectronics 7 (1992) 405.
H. Morgan, H. Fukushima and D. M. Taylor, Thin Solid Films (1994) in press.
N. M.Green, Adv. Protein Chem., 29 (1975) 85.
C. E.Argarana, I. D.Kuntz, S.Birken, R.Axel and C. R.Cantor, Nucleic Acids Res. 14 (1986) 1871.
A.Pahler, W. A.Hendrickson, M. A.Gawinowicz Kolks, C. E.Argarana and C. r. Cantor, J. Biol. Chem., 282 (1987) 13933
P. C.Weber, D.Ohlendorf, J.Wendoloski and F.Salemme, Science 243 (1989) 85.
W. A.Hendrickson, A.Pahler, J.Smith, Y.Satow, E.Merrit and R. Phizackerley, Proc. Natl. Acad Sci. 86 (1989) 2190.
O.Livnah, E.Bayer, M.Wilchek and J.Sussman, Proc. Nat, Acad. Sci. USA, 90 (1993) 5076
R. J.Leatherbarrow, M.Stedman and T. N. C.Wells J. Mol Biol. 221 (1991) 361.
M. E.Welland, M. J.Miles, N.Lambert, V. J.Morris, J. H.Coombs and J. B. Pethica, Int J Bio Macromol 11 (1989) 29.
T. P.Beebe, T. E.Wilson Jr, D. F.Ogletree, J. E.Katz, R.Balhorn, M. b. Salmeron and W. J.Siekhaus, Science 243 (1989) 370.
J.Schneir, O.Marti, G.Remmers, D.Glaser, R.Sonnenfeld, B.Drake, P. K.Hansma and V.Elings, J. Vac. Sci. Technol. A, 6 (1987) 283.
G.Thompson, J.Shen, P.Connolly and J.Cooper, Nanobiology, 2 (1993) 43.
R.Emch, J.Nogami, M.Dovek, C.Lang and C.Quate, J. Appl. Phys. 65 (1989) 79.
L.Haussling, B.Michel, H.Ringsdort and H.Rohrer, Angew. Chem. 30 (1991) 569.
P.Connolly, J.Cooper, G. R.Moores, J.Shen and G.Thompson, Nanotechnology, 2 (1991) 160.
A. M.Baro, R.Miranda, J.Alaman, N.Garcia, G.Binnig, H.Rohrer, C. H.Gerber and J.Carrascosa, Nature, 315 (1985) 253.
W.Heckl and G.Binnig, in Proceedings of the International Conference of Scanning Tunnelling Microscopy 1991, (North Holland, Amsterdam, 1991) p. 1073.
J.Frommer, Angew. Chem. 31 (1992) 1298.
F. A.Armstrong, H. A. O.Hill and B. N.Oliver, J. Chem. Soc. Commun. Com. 629 (1984) 976.
P. C.Weber, M. W.Pantoliano and L. D.Thompson, Biochem. 31 (1992) 9350.
H.Shirahama and T.Suzawa, J. Colloid Interface Sci, 104 (1985) 104.
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Cooper, J.M., Shen, J., Young, F.M. et al. The imaging of streptavidin and avidin using scanning tunnelling microscopy. J Mater Sci: Mater Electron 5, 106–110 (1994). https://doi.org/10.1007/BF00187121
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DOI: https://doi.org/10.1007/BF00187121