Abstract
Three alleles of the Zw locus of Drosophila melanogaster—Zw A, ZwB,and Zw lol—apparently code for dimeric, tetrameric, and monomeric forms of glucose-6-phosphate dehydrogenase (G6PD), respectively. The three forms of G6PD are characterized by different apparent K mvalues for glucose-6-phosphate but similar apparent K mvalues for NAPD+. When high concentrations of NAPD+ were added to enzyme preparations, the Zw Aand Zw lolforms of G6PD assumed tetrameric and dimeric properties, respectively. Although Zw loladults exhibit little G6PD activity, they maintain levels of G6PD-antigen comparable to those in Zw Aand Zw Badults. Thus the low level of G6PD activity in Zw lolindividuals cannot be explained as the consequence of lack of synthesis of the G6PD subunit.
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Bentley, M. M., Williamson, J. H., and Oliver, M. J. (1981). The effects of molybdate, tungstate and lxd on aldehyde oxidase and xanthine dehydrogenase in Drosophila melanogaster. Can. J. Genet. Cytol. 23597.
Bijlsma, R., and van der Meulen-Bruijns, C. (1979). Polymorphism at the G6PD and 6PGD loci in Drosophila melanogaster. III. Developmental and biochemical aspects. Biochem. Genet. 171131.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72248.
Browder, L. W., and Williamson, J. H. (1976). The effects of cinnamon on xanthine dehydrogenase, aldehyde oxidase and pyridoxal oxidase activity during development in Drosophila melanogaster. Dev. Biol. 53241.
Cochrane, B. J., and Lucchesi, J. C. (1980). Genetic variation in the effects of dietary sucrose on G6PD and 6PGD in Drosophila melanogaster larvae. Genetics 94:s20.
Fadda, S., Sangiorgi, S., and Pieragostini, E. (1979). Developmental G6PD polymorphism in Drosophila melanogaster: Evidence for nonstructural variants. Experientia 351441.
Geer, B. W., Bowman, J. T., and Simmons, J. R. (1974). The pentose shunt in wild-type and glucose-6-phosphate dehydrogenase deficient Drosophila melanogaster. J. Exp. Zool. 18777.
Geer, B. W., Kamiak, S. N., Kidd, K. R., Nishimura, R. A., and Yemm, S. J. (1976a). Regulation of the oxidative NADP-enzyme tissue levels in Drosophila melanogaster. I. Modulation by carbohydrate and lipid. J. Exp. Zool. 19515.
Geer, B. W., Kamiak, S. N., Kidd, K. R., Nishimura, R. A., and Yemm, S. J. (1976b). Regulation of the oxidative NADP-enzyme tissue levels in Drosophila melanogaster. II. The biochemical basis of dietary carbohydrate and D-glycerate modulation. J. Exp. Zool. 203391.
Geer, B. W., Krochko, D., and Williamson, J. H. (1979). Ontogeny, cell distribution and the physiological role of NADP-malic enzyme in Drosophila melanogaster. Biochem. Genet. 17867.
Geer, B. W., Lindel, D. L., and Lindel, D. M. (1979). Relationship of the oxidative pentose shunt pathway to lipid synthesis in Drosophila melanogaster. Biochem. Genet. 17881.
Geer, B. W., Williamson, J. H., Cavener, D. R., and Cochrane, B. J. (1981). Dietary modulation of glucose-6-phosphate and 6-phosphogluconate dehydrogenase in Drosophila. In Bhaskaran, G., Friedman, S., and Rodriguez, J. G. (eds.), Current Topics in Insect Endocrinology and Nutrition Plenum, New York, pp. 253–281.
Giesel, J. T. (1976). Biology of a duplicate gene system with glucose-6-phosphate dehydrogenase activity in Drosophila melanogaster: Genetic analysis and differences in fitness components and reaction to environmental parameters among Zw genotypes. Biochem. Genet. 14823.
Greenbaum, A. L., Gumaa, K. A., and McLean, P. (1971). The distribution of hepatic metabolites and the control of the pathways of carbohydrate metabolism in animals of different dietary and hormonal status. Arch. Biochem. Biophys. 143617.
Hori, S. H., and Tanda, S. (1980). Purification and properties of wild-type and mutant glucose-6-phosphate dehydrogenases and 6-phosphogluconate dehydrogenase from Drosophila melanogaster. Jap. J. Genet. 55211.
Hori, S. H., and Tanda, S. (1981). Genetic variations in the activities of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in Drosophila melanogaster. Evidence for an autosomal modifier system. Jap. J. Genet. 56257.
Huggins, G., and Yao, F. (1959). Influence of hormones on liver. I. Effects of steroids and thyroxine on pyridine nycleotide linked dehydrogenases. J. Exp. Med. 110899.
Kanungo, M. S., and Prosser, C. L. (1959). Physiological and biochemical adaption of goldfish to cold and warm temperatures. II. Oxygen consumption of liver homogenates and oxidative phosphorylation of liver mitochondria. J. Cell. Comp. Physiol. 54265.
Laurell, C. B. (1966). Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 1545.
Laurie-Ahlberg, C. C., Maroni, G., Bewley, G. C., Luccnesi, J. C., and Weir, B. S. (1980). Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 771073.
Laurie-Ahlberg, C. C., Williamson, J. H., Cochrane, B. J., Wilton, A. N., and Chasalow, F. I. (1981). Autosomal factors with correlated effects on the activities of the glucose-6-phosphate and 6-phosphogluconate dehydrogenases in Drosophila melanogaster. Genetics 99127.
Lee, C.-Y., Langley, C. H., and Burkhart, J. (1978). Purification and molecular weight determination of glucose-6-phosphate dehydrogenase and malic enzyme from mouse and Drosophila. Anal. Biochem. 86697.
Levy, H. R. (1979). Glucose-6-phosphate dehydrogenases. Adv. Enzymol. 4897.
Lewis, D. B. (1960). A new standard food medium. Dros. Inform. Serv. 34117.
Lindsley, D. L., and Grell, E. H. (1968). Genetic variation of Drosophila melanogaster. Carnegie Inst. Washington, Publ. No. 627.
Lucchesi, J. C. (1978). Gene dosage compensation and the evolution of sex chromosomes. Science 101711.
Lucchesi, J. C., Hughes, M. B., and Geer, B. W. (1979). Genetic control of pentose phosphate pathway enzymes in Drosophila. Curr. Topics Cell. Reg. 15143.
Meidinger, E. M., and Williamson J. H. (1978). Genetic control of aldehyde oxidase activity and cross-reacting-material in Drosophila melanogaster. Can. J. Genet. Cytol. 20489.
Pieragostini, E., Vanelli, M. L., Sangiorgi, S., and Palenzona, D. (1978). Glucose-6-phosphate dehydrogenase in Drosophila melanogaster: Autosomal determination and relationship to vg marker. Dros. Inform. Serv. 53189.
Steele, M. W., Young, W. J., and Childs, B. (1968). Glucose-6-phosphate dehydrogenase in Drosophila melanogaster: Starch gel electrophoretic variation due to molecular instability. Biochem. Genet. 2159.
Steele, M. W., Young, W. J., and Childs, B. (1969). Genetic regulation of glucose-6-phosphate dehydrogenase activity in Drosophila melanogaster. Biochem. Genet. 3359.
Stewart, B. R., and Merriam, J. R. (1974). Segmental aneuploidy and enzyme activity as a method for cytogenetic localization in Drosophila melanogaster. Genetics 76301.
Veeger, C., Der Vartanian, D. V., and Zeylemaker, W. P. (1969). Succinate dehydrogenase. In Lowenstein, J. M. (ed.), Methods in Enzymology, Vol. 8 Academic Press, New York, pp. 81–90.
Voelker, R. A., Langley, C. H., Leigh-Brown, A. J., Ohnishi, S., Dickson, B., Montgomery, E., and Smith, S. C., (1980). Enzyme null alleles in natural populations of Drosophila melanogaster: Frequencies in a North Carolina population. Proc. Natl. Acad. Sci. USA 771091.
Williamson, J. H., and Bentley, M. M., (1981). Dosage compensation in Drosophila melanogaster as illustrated by glucose 6-phosphate (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) specific activities and CRM levels. Genetics 97:s113.
Williamson, J. H., and Bentley, M. M. (1983). Dosage compensation in Drosophila: NADP-enzyme activities and cross-reacting material. Genetics 103649.
Young, W. J., Porter, J. E., and Childs, B. (1964). Glucose-6-phosphate dehydrogense in Drosophila. X-linked electrophoretic variants. Science 143 140.
Yugari, Y., and Matsuda, T., (1967). Glucose-6-phosphate dehydrogenase from rat liver II. Effect of diet on enzyme activity in vivo and inhibition by long chain fatty acids in vitro. J. Biochem. 61541.
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This work was supported by the Natural Sciences and Engineering Research Council of Canada and an Alberta Heritage Foundation for Medical Research Establishment Grant to M.M.B.
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Williamson, J.H., Bentley, M.M. Comparative properties of three forms of glucose-6-phosphate dehydrogenase in Drosophila melanogaster . Biochem Genet 21, 1153–1166 (1983). https://doi.org/10.1007/BF00488467
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DOI: https://doi.org/10.1007/BF00488467