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Components of the major urinary protein complex of inbred mice: Separation and peptide mapping

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Abstract

Gel filtration of the nondialyzable fraction of urine from normal inbred mice on Sephadex G-100 yielded three peaks (I, II, and III in order of elution), the relative sizes of which varied with the sex and strain of the mice. Constituents of peak I, the breakthrough peak, included uromucoid (Tamm-Horsfall mucoprotein); peak III was low in nitrogen, rich in carbohydrate, nonprecipitable with trichloroacetic acid, gave no definitive ultraviolet or visible spectrum, and had a sedimentation coefficient of 0.5 S. Peak II contained the electrophoretically distinguishable prealbumins of the major urinary protein (MUP) complex. These components (known as 1, 2, and 3 in order of increasing mobility toward the anode) were separated by chromatography on diethylaminoethyl cellulose. Tryptic peptide mapping indicated that components 1 and 2, a genetic variant shown to be under the control of one genetic locus (the Mup-alocus), differed by a single peptide. Components 1 and 3 had a number of peptides in common plus several peptides unique to each. The peptide map of any given component did not differ between sexes or between the strains investigated.

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References

  • Finlayson, J. S., and Baumann, C. A. (1958). Mouse proteinuria.Am. J. Physiol. 192 69.

    PubMed  Google Scholar 

  • Finlayson, J. S., Potter, M., and Runner, C. C. (1963). Electrophoretic variation and sex dimorphism of the major urinary protein complex in inbred mice: a new genetic marker.J. Natl. Cancer Inst. 31 91.

    PubMed  Google Scholar 

  • Finlayson, J. S., Asofsky, R., Potter, M., and Runner, C. C. (1965). Major urinary protein complex of normal mice: origin.Science 149 981.

    PubMed  Google Scholar 

  • Finlayson, J. S., and Morris, H. P. (1965). Molecular size of rat urinary protein.Proc. Soc. Exptl. Biol. Med. 119 663.

    Google Scholar 

  • Hudson, D. M., Finlayson, J. S., and Potter, M. (1967). Linkage of one component of the major urinary protein complex of mice to the brown coat color locus.Genet. Res. (Cambridge)10 195.

    Google Scholar 

  • Katz, A. M., Dreyer, W. J., and Anfinsen, C. B. (1959). Peptide separation by two-dimensional chromatography and electrophoresis.J. Biol. Chem. 234 2897.

    PubMed  Google Scholar 

  • Maxfield, M. (1961). Molecular forms of human urinary mucoprotein present under physiological conditions.Biochim. Biophys. Acta 49 548.

    PubMed  Google Scholar 

  • Peterson, E. A., and Sober, H. A. (1959). Variable gradient device for chromatography.Anal. Chem. 31 857.

    Google Scholar 

  • Potter, M., and Kuff, E. L. (1961). Myeloma globulins of plasma-cell neoplasms in inbred mice. I. Immunoelectrophoresis of serum, with rabbit antibodies prepared against microsome fractions of the neoplasms.J. Natl. Cancer Inst. 26 1109.

    PubMed  Google Scholar 

  • Potter, M., Dreyer, W. J., Kuff, E. L., and McIntire, K. R. (1964). Heritable variation in Bence Jones protein structure in an inbred strain of mice.J. Mol. Biol. 8 814.

    Google Scholar 

  • Rümke, P., and Thung, P. J. (1964). Immunological studies on the sex-dependent prealbumin in mouse urine and its occurrence in the serum.Acta Endocrinol. 47 156.

    PubMed  Google Scholar 

  • Schachman, H. K. (1957). Ultracentrifugation, diffusion, and viscometry. InMethods in Enzymology, Vol. 4. Edited by S. P. Colowick and N. O. Kaplan. Academic Press, New York, pp. 32–104.

    Google Scholar 

  • Thung, P. J. (1956). Proteinuria in mice and its relevance to comparative gerontology.Experientia Suppl. 4 195.

    Google Scholar 

  • Thung, P. J. (1962). Physiological proteinuria in mice.Acta Physiol. Pharmacol. Neerl. 10 248.

    PubMed  Google Scholar 

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Finlayson, J.S., Mushinski, J.F., Hudson, D.M. et al. Components of the major urinary protein complex of inbred mice: Separation and peptide mapping. Biochem Genet 2, 127–140 (1968). https://doi.org/10.1007/BF01458712

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