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UV damage of T2-phage particles and conformation of DNA in situ

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Il Nuovo Cimento D

Summary

The effect of UV irradiation on in situ and free T2 DNA is studied by analysing the melting profiles and absorption spectra modifications. The similar photochemical behaviour obtained in the two systems seems to exclude significant differences in their secondary structure.

Riassunto

Si studia l'effetto dell'irraggiamento UV sul DNA del fago T2 in situ e libero in soluzione mediante un'analisi delle modificazioni dei profili di fusione e degli spettri d'assorbimento. L'analogo comportamento fotochimico osservato nei due sistemi sembra escludere significative differenze nella loro struttura secondaria.

Резюме

Исследуется влияние ультрафиолетового облучения на ДНК фага T2 на месте и на свободные молекулы ДНК в растворе, посредством анализа изменений профилей плавления и изменений спектров поглощения. Аналогичное фотохимическое ооведение, полученное в этих двух системах, по-видимому, исключает значительные различия между их вторичными структурами.

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Authors and Affiliations

  1. Laboratorio di Fisica, Istituto Superiore di Sanità, Roma

    M. Matzeu & O. M. Quaranta

  2. Istituto di Fisica dell'Università, Perugia

    G. Onori

Authors
  1. M. Matzeu
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  2. O. M. Quaranta
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  3. G. Onori
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Matzeu, M., Quaranta, O.M. & Onori, G. UV damage of T2-phage particles and conformation of DNA in situ . Il Nuovo Cimento D 2, 1489–1496 (1983). https://doi.org/10.1007/BF02460227

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  • DOI: https://doi.org/10.1007/BF02460227

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